Team:Sharon MA Aquila/Project

From 2012hs.igem.org

(Difference between revisions)
(→Parts)
 
(5 intermediate revisions not shown)
Line 2: Line 2:
===Parts===
===Parts===
-
*[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC)
+
[http://http://partsregistry.org/Part:BBa_K116001 Our pH sensor]
-
*[http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001)
+
-
*[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006)
+
===Materials===
===Materials===
Line 12: Line 10:
*'''Bacterial Transformation:'''
*'''Bacterial Transformation:'''
**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
-
**Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)
+
**Erythromycin (for selection)
-
**Erythromycin, Chloramphenicol, or other antibiotics (for selection)
+
**Gene Pulser and Pulse Controller Electrophoration apparatus
**Gene Pulser and Pulse Controller Electrophoration apparatus
-
**Gaspak or method to generate anaerobic conditions
+
**Gaspak or method to generate anaerobic conditions (airtight box with lit candles)
*'''Miniprep'''
*'''Miniprep'''
-
**TES(50mM Tris[pH 7]
+
**TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose)
-
**20mM EDTA, 0.2 mM sucrose)
+
**10mg/ml lysozyme
**10mg/ml lysozyme
**70U/ml mutanolysin
**70U/ml mutanolysin

Latest revision as of 19:37, 21 May 2013


Parts

[http://http://partsregistry.org/Part:BBa_K116001 Our pH sensor]

Materials

  • Culturing:
    • [http://www.bd.com/ds/productCenter/288110.asp MRS broth]
    • [http://www.bd.com/ds/productCenter/288210.asp MRS agar]
  • Bacterial Transformation:
    • Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
    • Erythromycin (for selection)
    • Gene Pulser and Pulse Controller Electrophoration apparatus
    • Gaspak or method to generate anaerobic conditions (airtight box with lit candles)
  • Miniprep
    • TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose)
    • 10mg/ml lysozyme
    • 70U/ml mutanolysin


<forum_subtle />
Retrieved from "http://2012hs.igem.org/Team:Sharon_MA_Aquila/Project"