http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&feed=atom&action=history
Team:Sharon MA Aquila/Project - Revision history
2024-03-28T12:59:26Z
Revision history for this page on the wiki
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Aalekseyeva: /* Parts */
2013-05-21T19:37:56Z
<p><span class="autocomment">Parts</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:37, 21 May 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Parts===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Parts===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*</del>[http://<del class="diffchange diffchange-inline">www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[http://http://partsregistry.org/Part:<ins class="diffchange diffchange-inline">BBa_K116001 Our pH sensor</ins>]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*[http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*[</del>http://partsregistry.org/Part:<del class="diffchange diffchange-inline">BBa_K128007 Plasmid</del>] <del class="diffchange diffchange-inline">to confer Erythromycin resistance to bacteria (from the Parts Registry BBa_K128007)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Materials===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Materials===</div></td></tr>
</table>
Aalekseyeva
http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&diff=1486&oldid=prev
Hannahb at 01:01, 4 April 2012
2012-04-04T01:01:57Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 01:01, 4 April 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K128007 Plasmid] to confer Erythromycin resistance to bacteria</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K128007 Plasmid] to confer Erythromycin resistance to bacteria <ins class="diffchange diffchange-inline">(from the Parts Registry BBa_K128007)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Materials===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Materials===</div></td></tr>
</table>
Hannahb
http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&diff=1469&oldid=prev
Hannahb at 19:45, 3 April 2012
2012-04-03T19:45:11Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:45, 3 April 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions (airtight box with lit candles)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions (airtight box with lit candles)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Miniprep'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Miniprep'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>**TES(50mM Tris[pH 7]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>**TES(50mM Tris[pH 7]<ins class="diffchange diffchange-inline">, </ins>20mM EDTA, 0.2 mM sucrose)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">**</del>20mM EDTA, 0.2 mM sucrose)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**10mg/ml lysozyme</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**10mg/ml lysozyme</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**70U/ml mutanolysin</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**70U/ml mutanolysin</div></td></tr>
</table>
Hannahb
http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&diff=1467&oldid=prev
Hannahb at 19:35, 3 April 2012
2012-04-03T19:35:25Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:35, 3 April 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Bacterial Transformation:'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Bacterial Transformation:'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">**Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Erythromycin (for selection)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Erythromycin (for selection)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gene Pulser and Pulse Controller Electrophoration apparatus</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gene Pulser and Pulse Controller Electrophoration apparatus</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions <ins class="diffchange diffchange-inline">(airtight box with lit candles)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Miniprep'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Miniprep'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**TES(50mM Tris[pH 7]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**TES(50mM Tris[pH 7]</div></td></tr>
</table>
Hannahb
http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&diff=1466&oldid=prev
Hannahb at 19:32, 3 April 2012
2012-04-03T19:32:26Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:32, 3 April 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>**Erythromycin<del class="diffchange diffchange-inline">, Chloramphenicol, or other antibiotics </del>(for selection)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>**Erythromycin (for selection)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gene Pulser and Pulse Controller Electrophoration apparatus</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gene Pulser and Pulse Controller Electrophoration apparatus</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions</div></td></tr>
</table>
Hannahb
http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&diff=1464&oldid=prev
Hannahb at 19:25, 3 April 2012
2012-04-03T19:25:42Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:25, 3 April 2012</td>
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<td colspan="2" class="diff-lineno">Line 5:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[http://partsregistry.org/Part:BBa_K128007 Plasmid] to confer Erythromycin resistance to bacteria</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Materials===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Materials===</div></td></tr>
</table>
Hannahb
http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&diff=1463&oldid=prev
Hannahb at 19:21, 3 April 2012
2012-04-03T19:21:47Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:21, 3 April 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 13:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>**Erythromycin, Chloramphenicol, other antibiotics <del class="diffchange diffchange-inline">may be needed </del>for selection</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>**Erythromycin, Chloramphenicol, <ins class="diffchange diffchange-inline">or </ins>other antibiotics <ins class="diffchange diffchange-inline">(</ins>for selection<ins class="diffchange diffchange-inline">)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gene Pulser and Pulse Controller Electrophoration apparatus</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gene Pulser and Pulse Controller Electrophoration apparatus</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions</div></td></tr>
</table>
Hannahb
http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&diff=1462&oldid=prev
Fzhou1995: /* Parts */
2012-04-03T19:20:47Z
<p><span class="autocomment">Parts</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:20, 3 April 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Parts===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Parts===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K652001 RiAFP <del class="diffchange diffchange-inline">part</del>] (from the Parts Registry)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K652001 RiAFP <ins class="diffchange diffchange-inline">Part</ins>] (from the Parts Registry <ins class="diffchange diffchange-inline">BBa_K652001</ins>)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Materials===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Materials===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Culturing:'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Culturing:'''</div></td></tr>
</table>
Fzhou1995
http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&diff=1461&oldid=prev
Hannahb at 19:17, 3 April 2012
2012-04-03T19:17:59Z
<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:17, 3 April 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gene Pulser and Pulse Controller Electrophoration apparatus</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gene Pulser and Pulse Controller Electrophoration apparatus</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Gaspak or method to generate anaerobic conditions</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*'''Miniprep'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**TES(50mM Tris[pH 7]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**20mM EDTA, 0.2 mM sucrose)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**10mg/ml lysozyme</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**70U/ml mutanolysin</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><forum_subtle /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><forum_subtle /></div></td></tr>
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Hannahb
http://2012hs.igem.org/wiki/index.php?title=Team:Sharon_MA_Aquila/Project&diff=1460&oldid=prev
Hannahb at 19:15, 3 April 2012
2012-04-03T19:15:46Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:15, 3 April 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Aquila}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Aquila}}</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>===Parts<del class="diffchange diffchange-inline">:</del>===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>===Parts===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K652001 RiAFP part] (from the Parts Registry)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/Part:BBa_K652001 RiAFP part] (from the Parts Registry)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*''Culturing''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">===Materials===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">'</ins>''Culturing<ins class="diffchange diffchange-inline">:'</ins>''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**[http://www.bd.com/ds/productCenter/288110.asp MRS broth]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**[http://www.bd.com/ds/productCenter/288110.asp MRS broth]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**[http://www.bd.com/ds/productCenter/288210.asp MRS agar] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**[http://www.bd.com/ds/productCenter/288210.asp MRS agar] </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*'''Bacterial Transformation:'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**Erythromycin, Chloramphenicol, other antibiotics may be needed for selection</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**Gene Pulser and Pulse Controller Electrophoration apparatus</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**Gaspak or method to generate anaerobic conditions</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><forum_subtle /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><forum_subtle /></div></td></tr>
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Hannahb