Team:Sharon MA Aquila/Project
From 2012hs.igem.org
(Difference between revisions)
Line 13: | Line 13: | ||
*'''Bacterial Transformation:''' | *'''Bacterial Transformation:''' | ||
**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6) | **Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6) | ||
- | |||
**Erythromycin (for selection) | **Erythromycin (for selection) | ||
**Gene Pulser and Pulse Controller Electrophoration apparatus | **Gene Pulser and Pulse Controller Electrophoration apparatus | ||
- | **Gaspak or method to generate anaerobic conditions | + | **Gaspak or method to generate anaerobic conditions (airtight box with lit candles) |
*'''Miniprep''' | *'''Miniprep''' | ||
**TES(50mM Tris[pH 7] | **TES(50mM Tris[pH 7] |
Revision as of 19:35, 3 April 2012
Parts
- Lactobacillus delbrueckii subsp. bulgaricus (from ATCC)
- RiAFP Part (from the Parts Registry BBa_K652001)
- LacS Promoter (from the Parts Registry BBa_K128006)
- Plasmid to confer Erythromycin resistance to bacteria
Materials
- Culturing:
- Bacterial Transformation:
- Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
- Erythromycin (for selection)
- Gene Pulser and Pulse Controller Electrophoration apparatus
- Gaspak or method to generate anaerobic conditions (airtight box with lit candles)
- Miniprep
- TES(50mM Tris[pH 7]
- 20mM EDTA, 0.2 mM sucrose)
- 10mg/ml lysozyme
- 70U/ml mutanolysin