Team:Lethbridge Canada/The Project
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Mlc is a repressive protein that binds to a specific DNA sequence and prevents it from being transcribed. This regulates the transcription of specific genes so certain proteins are only made when they are needed. This is the mechanism we are going to use to regulate the transcription of the gene for insulin. When glucose enters the cells via enzyme II, it picks up a phosphoryl group that was originally bound to EIICBGlc. The dephosphorylated form of EIICBGlc has a high affinity with Mlc and recruits MCL from its binding site on the DNA sequence. The gene is now free to be transcribed. Due to the fact that this sequence is originally stimulated by an increase in glucose concentration as it enters the cell, it is the perfect circuit for us to use to regulate the production of inulin. | Mlc is a repressive protein that binds to a specific DNA sequence and prevents it from being transcribed. This regulates the transcription of specific genes so certain proteins are only made when they are needed. This is the mechanism we are going to use to regulate the transcription of the gene for insulin. When glucose enters the cells via enzyme II, it picks up a phosphoryl group that was originally bound to EIICBGlc. The dephosphorylated form of EIICBGlc has a high affinity with Mlc and recruits MCL from its binding site on the DNA sequence. The gene is now free to be transcribed. Due to the fact that this sequence is originally stimulated by an increase in glucose concentration as it enters the cell, it is the perfect circuit for us to use to regulate the production of inulin. | ||
- | Nam, T., Cho, S., Shin, D., Kim, J., Jeong, J., Lee, J., Roe, J., & Kang, S. (2001). The | + | Nam, T., Cho, S., Shin, D., Kim, J., Jeong, J., Lee, J., Roe, J., & Kang, S. (2001). The Escherichia coli glucose transport enzyme iicb(glc) recruits the global repressor mlc. Retrieved from http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=historysearch&querykey=1 |
Revision as of 23:20, 13 June 2012
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ProjectDiabetes
Sensory transduction is a mechanism used by many organisms to monitor their external environments. E. coli detects changes in glucose levels in its environment by means of phosphotransferase system (PTS). This system uses E. coli’s enzyme II, which consists of the membrane-bound protein EIICBGlc, as well as enzyme I (EI), a histidine phosphocarrier protein HPr, and another protein called EIIAGlc. Enzyme II is responsible for the phosphorylation and transportation of glucose into the cell. Mlc is a repressive protein that binds to a specific DNA sequence and prevents it from being transcribed. This regulates the transcription of specific genes so certain proteins are only made when they are needed. This is the mechanism we are going to use to regulate the transcription of the gene for insulin. When glucose enters the cells via enzyme II, it picks up a phosphoryl group that was originally bound to EIICBGlc. The dephosphorylated form of EIICBGlc has a high affinity with Mlc and recruits MCL from its binding site on the DNA sequence. The gene is now free to be transcribed. Due to the fact that this sequence is originally stimulated by an increase in glucose concentration as it enters the cell, it is the perfect circuit for us to use to regulate the production of inulin. Nam, T., Cho, S., Shin, D., Kim, J., Jeong, J., Lee, J., Roe, J., & Kang, S. (2001). The Escherichia coli glucose transport enzyme iicb(glc) recruits the global repressor mlc. Retrieved from http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=historysearch&querykey=1
Insulin Production and Secretion
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