Team:Lethbridge Canada/Results
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<tr><td>[http://partsregistry.org/Part:BBa_K736003 BBa_K736003]</td><td>Composite</td><td>pLAC-regulated TAT-RFP construct</td><td>948 bp</td></tr> | <tr><td>[http://partsregistry.org/Part:BBa_K736003 BBa_K736003]</td><td>Composite</td><td>pLAC-regulated TAT-RFP construct</td><td>948 bp</td></tr> | ||
<tr><td>[http://partsregistry.org/Part:BBa_K736004 BBa_K736004]</td><td>Coding</td><td> | <tr><td>[http://partsregistry.org/Part:BBa_K736004 BBa_K736004]</td><td>Coding</td><td> | ||
- | Coding sequence for insulin chain A</td><td> | + | Coding sequence for insulin chain A</td><td>72 bp</td></tr> |
<tr><td>[http://partsregistry.org/Part:BBa_K736005 BBa_K736005]</td><td>Coding</td><td> | <tr><td>[http://partsregistry.org/Part:BBa_K736005 BBa_K736005]</td><td>Coding</td><td> | ||
- | Coding sequence for insulin chain B</td><td> | + | Coding sequence for insulin chain B</td><td>99 bp</td></tr> |
Revision as of 03:35, 17 June 2012
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ResultsParts createdWe created two new parts, BBa_K736000 (which regulated the transcription of downstream genes) and BBa_K736001 (which was a TAT signal sequence fused to a red fluorescent protein, E1010). We also assembled two parts, BBa_K736002 (which tests the ability of glucose to regulate transcription and uses enhanced RFP as the reporter) and BBa_K736003 (which tests for the ability of the TAT sequence to cause secretion of protein outside of the cell and also uses RFP as a reporter). We also created one part that coded for human insulin chain A (BBa_K736004) and one part that coded for human insulin chain B (BBa_K736005).
Proof of PrincipleRFP with Cells Media at Different Induction Levels
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