Results

Parts created

We created two new parts, BBa_K736000 (which regulated the transcription of downstream genes) and BBa_K736001 (which was a TAT signal sequence fused to a red fluorescent protein, E1010). We also assembled two parts, BBa_K736002 (which tests the ability of glucose to regulate transcription and uses enhanced RFP as the reporter) and BBa_K736003 (which tests for the ability of the TAT sequence to cause secretion of protein outside of the cell and also uses RFP as a reporter). We also created one part that coded for human insulin chain A (BBa_K736004) and one part that coded for human insulin chain B (BBa_K736005).

Name	Type	Description	Length
BBa_K736000	Regulatory	Constitutive Promoter (J23100) with MLC (glucose-regulated) binding site	36 bp
BBa_K736001	Reporter	TAT signal sequence fused to red fluorescent protein (E1010)	720 bp
BBa_K736002	Composite	Constitutive Promoter with MLC-binding-site-regulated enhanced RFP	913 bp
BBa_K736003	Composite	pLAC-regulated TAT-RFP construct	948 bp
BBa_K736004	Coding	Coding sequence for insulin chain A	72 bp
BBa_K736005	Coding	Coding sequence for insulin chain B	99 bp

Proof of Principle

RFP with Cells Media at Different Induction Levels

Glucose Variable Concentrations