Team:Lethbridge Canada/Results

From 2012hs.igem.org

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'''Glucose Variable Concentrations'''
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[[Image:Glucose variable concentrations 4.5 hours.JPG|left|px]]
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[[Image:Glucose variable concentrations 6 hours.JPG|left|px]]

Revision as of 03:52, 17 June 2012

HP.JPG
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Results

Parts created

We created two new parts, [http://partsregistry.org/Part:BBa_K736000 BBa_K736000] (which regulated the transcription of downstream genes) and [http://partsregistry.org/Part:BBa_K736001 BBa_K736001] (which was a TAT signal sequence fused to a red fluorescent protein, E1010). We also assembled two parts, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K736002 BBa_K736002] (which tests the ability of glucose to regulate transcription and uses enhanced RFP as the reporter) and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K736003 BBa_K736003] (which tests for the ability of the TAT sequence to cause secretion of protein outside of the cell and also uses RFP as a reporter). We also created one part that coded for human insulin chain A ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K736004 BBa_K736004]) and one part that coded for human insulin chain B ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K736005 BBa_K736005]).



NameTypeDescriptionLength
[http://partsregistry.org/Part:BBa_K736000 BBa_K736000]RegulatoryConstitutive Promoter (J23100) with MLC (glucose-regulated) binding site36 bp
[http://partsregistry.org/Part:BBa_K736001 BBa_K736001]ReporterTAT signal sequence fused to red fluorescent protein (E1010)720 bp
[http://partsregistry.org/Part:BBa_K736002 BBa_K736002]CompositeConstitutive Promoter with MLC-binding-site-regulated enhanced RFP913 bp
[http://partsregistry.org/Part:BBa_K736003 BBa_K736003]CompositepLAC-regulated TAT-RFP construct948 bp
[http://partsregistry.org/Part:BBa_K736004 BBa_K736004]Coding Coding sequence for insulin chain A72 bp
[http://partsregistry.org/Part:BBa_K736005 BBa_K736005]Coding Coding sequence for insulin chain B99 bp


Proof of Principle

RFP with Cells Media at Different Induction Levels


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Glucose Variable Concentrations


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