http://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&feed=atom&action=historyTeam:Heidelberg LSL/Project Summary - Revision history2024-03-28T20:22:25ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9864&oldid=prevHetitus at 03:34, 17 June 20122012-06-17T03:34:03Z<p></p>
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</table>Hetitushttp://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9701&oldid=prevDniopek at 03:00, 17 June 20122012-06-17T03:00:09Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h4>Lying in the sun we now have time to recover and think about the past weeks…</h4></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h4>Lying in the sun<ins class="diffchange diffchange-inline">, </ins>we now have time to recover and think about the past weeks…</h4></div></td></tr>
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</table>Dniopekhttp://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9698&oldid=prevDniopek at 02:59, 17 June 20122012-06-17T02:59:09Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We are looking forward to the iGem HS Jamboree 2012 and <del class="diffchange diffchange-inline">present </del>our work to the other teams from all over the world. We hope to get <del class="diffchange diffchange-inline">in </del>contact with other <del class="diffchange diffchange-inline">biology interested </del>pupils <del class="diffchange diffchange-inline">and present them our iGEMS</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We are looking forward to the iGem HS Jamboree 2012 and <ins class="diffchange diffchange-inline">to presenting </ins>our work to the other teams from all over the world. We hope to get <ins class="diffchange diffchange-inline">into </ins>contact with other pupils <ins class="diffchange diffchange-inline">interested in synthetic biology</ins>.</div></td></tr>
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</table>Dniopekhttp://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9688&oldid=prevDniopek at 02:57, 17 June 20122012-06-17T02:57:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Summary</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Summary</h4></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV<del class="diffchange diffchange-inline">-</del>radiation in both artificial, and natural doses of radiation. <br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV radiation in both artificial, and natural doses of radiation. <br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV<del class="diffchange diffchange-inline">-</del>detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>With this unpredictable success, we are able to embed this new and innovative UV<del class="diffchange diffchange-inline">-</del>detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>With this unpredictable success, we are able to embed this new and innovative UV detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We have worked with enthusiasm on the bacterial tool kit which can detect UV<del class="diffchange diffchange-inline">-</del>radiation and have made a lot of progress, therefore we want to continue working on it.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We have worked with enthusiasm on the bacterial tool kit which can detect UV radiation and have made a lot of progress, therefore we want to continue working on it.</div></td></tr>
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</table>Dniopekhttp://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9680&oldid=prevDniopek at 02:55, 17 June 20122012-06-17T02:55:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people from too intense UV radiation but also are trendy accessories! </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people from too intense UV radiation but also are trendy accessories! </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.</del></div></td><td colspan="2"> </td></tr>
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</table>Dniopekhttp://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9676&oldid=prevDniopek at 02:55, 17 June 20122012-06-17T02:55:18Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The system still has to be optimized for real life application. Therefore, it has to be adapted to the sensitivity of mammalian cells towards UV-induced DNA damage. Among other things this would include optimization of the bacterial container which might absorb a certain amount of UV radiation. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The system still has to be optimized for real life application. Therefore, it has to be adapted to the sensitivity of mammalian cells towards UV-induced DNA damage. Among other things this would include optimization of the bacterial container which might absorb a certain amount of UV radiation. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people <del class="diffchange diffchange-inline">of </del>too intense UV<del class="diffchange diffchange-inline">-</del>radiation but also are trendy accessories! <del class="diffchange diffchange-inline">People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people <ins class="diffchange diffchange-inline">from </ins>too intense UV radiation but also are trendy accessories! </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.</div></td></tr>
</table>Dniopekhttp://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9668&oldid=prevDniopek at 02:52, 17 June 20122012-06-17T02:52:48Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">For the production of the jewelry, which shall </del>be <del class="diffchange diffchange-inline">once a common product </del>for <del class="diffchange diffchange-inline">the public, we need to simplify this system</del>. <del class="diffchange diffchange-inline">Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue</del>, <del class="diffchange diffchange-inline">when </del>it <del class="diffchange diffchange-inline">is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way </del>to <del class="diffchange diffchange-inline">recognize UV-radiation, which does not need any further background in synthetic biology. At </del>the <del class="diffchange diffchange-inline">moment our bacteria turn blue after 10 min exposure. But the human body can resist much more </del>UV-<del class="diffchange diffchange-inline">light without getting </del>DNA<del class="diffchange diffchange-inline">-</del>damage. <del class="diffchange diffchange-inline">We have to find a rec gene that show the most similarities with the human body. An </del>other <del class="diffchange diffchange-inline">possibility to handle </del>this <del class="diffchange diffchange-inline">problem </del>would <del class="diffchange diffchange-inline">be to vary </del>the <del class="diffchange diffchange-inline">UV permeability </del>of <del class="diffchange diffchange-inline">the bacteria containment</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The system still has to </ins>be <ins class="diffchange diffchange-inline">optimized </ins>for <ins class="diffchange diffchange-inline">real life application</ins>. <ins class="diffchange diffchange-inline">Therefore</ins>, it <ins class="diffchange diffchange-inline">has to be adapted </ins>to the <ins class="diffchange diffchange-inline">sensitivity of mammalian cells towards </ins>UV-<ins class="diffchange diffchange-inline">induced </ins>DNA damage. <ins class="diffchange diffchange-inline">Among </ins>other <ins class="diffchange diffchange-inline">things </ins>this would <ins class="diffchange diffchange-inline">include optimization of </ins>the <ins class="diffchange diffchange-inline">bacterial container which might absorb a certain amount </ins>of <ins class="diffchange diffchange-inline">UV radiation</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Some more problems that came out while working on the constructs. </del>At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. </div></td></tr>
</table>Dniopekhttp://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9618&oldid=prevDniopek at 02:46, 17 June 20122012-06-17T02:46:31Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not sure <del class="diffchange diffchange-inline">if </del>precA <del class="diffchange diffchange-inline">becomes </del>activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work <del class="diffchange diffchange-inline">as </del>the <del class="diffchange diffchange-inline">precA </del>promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment<del class="diffchange diffchange-inline">. But we have got a contact person who could give us access to the required machines</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not <ins class="diffchange diffchange-inline">for </ins>sure <ins class="diffchange diffchange-inline">whether </ins>precA <ins class="diffchange diffchange-inline">is </ins>activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work <ins class="diffchange diffchange-inline">because </ins>the <ins class="diffchange diffchange-inline">recA </ins>promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We also want to include the constructs with a GFP reporter instead of LacZ in our <del class="diffchange diffchange-inline">iGEMs </del>collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further <del class="diffchange diffchange-inline">measurement </del>systems <del class="diffchange diffchange-inline">to detect </del>GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm. Therefore we can make GFP visible using blue LEDs. Furthermore we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. <del class="diffchange diffchange-inline">We </del>could generate a color range from green to orange to red with just one single blue LED.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We also want to include the constructs with a GFP reporter instead of LacZ in our <ins class="diffchange diffchange-inline">iGEMS </ins>collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further <ins class="diffchange diffchange-inline">detection </ins>systems <ins class="diffchange diffchange-inline">for </ins>GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm <ins class="diffchange diffchange-inline">which is the excitation wavelength of GFP</ins>. Therefore<ins class="diffchange diffchange-inline">, </ins>we can make GFP visible using blue LEDs. Furthermore<ins class="diffchange diffchange-inline">, </ins>we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. <ins class="diffchange diffchange-inline">By using different fluorescent reporters we </ins>could generate a color range from green to orange to red with just one single blue LED.</div></td></tr>
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</table>Dniopekhttp://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9597&oldid=prevDniopek at 02:41, 17 June 20122012-06-17T02:41:03Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun <del class="diffchange diffchange-inline">expose </del>than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun <ins class="diffchange diffchange-inline">exposure </ins>than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For us<ins class="diffchange diffchange-inline">, </ins>personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Outlook</h4></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h4>Outlook</h4></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But <del class="diffchange diffchange-inline">furthermore </del>we have <del class="diffchange diffchange-inline">found </del>difficulties that <del class="diffchange diffchange-inline">should </del>be <del class="diffchange diffchange-inline">optimised </del>in the future.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But<ins class="diffchange diffchange-inline">, nevertheless, </ins>we have <ins class="diffchange diffchange-inline">encountered </ins>difficulties that <ins class="diffchange diffchange-inline">could </ins>be <ins class="diffchange diffchange-inline">optimized </ins>in the future.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We have worked with enthusiasm on the <del class="diffchange diffchange-inline">bacteria </del>tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We have worked with enthusiasm on the <ins class="diffchange diffchange-inline">bacterial </ins>tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In the future we want to create a clearer <del class="diffchange diffchange-inline">characterisation </del>of different members of the same gene family as precA. <del class="diffchange diffchange-inline">So </del>Our assumption is that <del class="diffchange diffchange-inline"> </del>different rec genes are activated <del class="diffchange diffchange-inline">based on a certain characteristic extension of DNA damage </del>dependent on UV radiation. By <del class="diffchange diffchange-inline">linking </del>these genes <del class="diffchange diffchange-inline">together </del>we want to generate a more gradual UV-response <del class="diffchange diffchange-inline">for </del>the <del class="diffchange diffchange-inline">detection of qualitative UV-intensity. At UV-intensities </del>different <del class="diffchange diffchange-inline">genes are activated and a color gradient appears. This gradient will be used </del>to determine the exact amount of radiation. This is important to <del class="diffchange diffchange-inline">give </del>the <del class="diffchange diffchange-inline">bacteria </del>tool kit the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and <del class="diffchange diffchange-inline">precC, they already gave us </del>a positive result, <del class="diffchange diffchange-inline">so that our assumption was right. Now we have to analyse </del>the <del class="diffchange diffchange-inline">data to find their most effective use</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In the future we want to create a clearer <ins class="diffchange diffchange-inline">characterization </ins>of different members of the same gene family as precA. Our assumption is that <ins class="diffchange diffchange-inline">DNA damage leads to the successive activation of the </ins>different rec genes are activated dependent on <ins class="diffchange diffchange-inline">the </ins>UV radiation <ins class="diffchange diffchange-inline">dose</ins>. By <ins class="diffchange diffchange-inline">implementing also </ins>these genes we want to generate a more gradual UV-response<ins class="diffchange diffchange-inline">. The successive induction of </ins>the different <ins class="diffchange diffchange-inline">rec promoters could help </ins>to determine the exact amount of radiation. This is important to <ins class="diffchange diffchange-inline">provide </ins>the <ins class="diffchange diffchange-inline">bacterial </ins>tool kit <ins class="diffchange diffchange-inline">with </ins>the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and <ins class="diffchange diffchange-inline">preC. PreB lead to </ins>a positive result, <ins class="diffchange diffchange-inline">whereas induction of preC did not result in </ins>the <ins class="diffchange diffchange-inline">generation of the reporter. Cloning of this part will be repeated</ins>. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not <del class="diffchange diffchange-inline">analysed if </del>the construct would also work with radioactive radiation. Radioactive radiation causes different DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not <ins class="diffchange diffchange-inline">analyzed whether </ins>the construct would also work with radioactive radiation. Radioactive radiation causes <ins class="diffchange diffchange-inline">DNA damage </ins>different <ins class="diffchange diffchange-inline">from UV-induced </ins>DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.</div></td></tr>
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</table>Dniopekhttp://2012hs.igem.org/wiki/index.php?title=Team:Heidelberg_LSL/Project_Summary&diff=9531&oldid=prevDniopek at 02:26, 17 June 20122012-06-17T02:26:28Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern and <del class="diffchange diffchange-inline">familiar background. As </del>a <del class="diffchange diffchange-inline">open-minded part </del>of <del class="diffchange diffchange-inline">the society, this should encourage the reader to think again about </del>synthetic biology <del class="diffchange diffchange-inline">and to begin controversial discussions about this upcoming field of science</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern <ins class="diffchange diffchange-inline">context </ins>and <ins class="diffchange diffchange-inline">give people </ins>a <ins class="diffchange diffchange-inline">better understanding </ins>of synthetic biology. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.</div></td></tr>
</table>Dniopek