Team:Heidelberg LSL/Parts

From 2012hs.igem.org

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<h2>Unveiling the Invisible</h2>
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<h4>Synthetic Measurement Toolkit for the precise Quantification of UV and radioactive Radiation</h4>
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<p>UV radiation and radioactivity are two natural radiation types we get in contact with every day. In low doses, UV and radioactive radiation are mostly harmless to cells and can even be beneficial for the survival of an organism. Though, when exceeding the healthy range, they can cause severe cellular damage, which may lead to diseases such as cancer in humans. The iGEM team Life-Science Lab Heidelberg has developed a synthetic measurement toolkit consisting of standardized parts for the precise quantification of both UV and radioactive radiation. Our toolkit is applicable in a variety of everyday life settings- from checking the exposure of your body to UV-light during sun-bathing to detecting sources of radioactivity in high-risk-areas. Finally, by exploring our toolkit in context of a real consumer product called “iGEMs”, we want to raise the public awareness for the invisible danger and exemplify the great perspectives offered by synthetic biology.
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Revision as of 16:48, 11 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

hllao

NameDescriptionRegistry linkPart typeAvailability
precA_LacZThis is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).

BBa_K862000

Measurementpartsregistry
psulA_LacZThis is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a SulA Promoter (part BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004). BBa_K862001 Measurement partsregistry
precB_LacZThis is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recB Promoter (part BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).BBa_K862002 Measurement partsregistry
precBThe recB promoter sequence was taken for the E. coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.
RecB_fw: aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA
CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc
RecB_rev: ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC
GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg
BBa_K862003 Regulatory Oligo sequence provided