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Team:Heidelberg LSL/Parts
From 2012hs.igem.org
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| - | <td>psulA_LacZ</td><td>This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a SulA Promoter (part BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004). <td><a href="http://partsregistry.org/Part: | + | <td>psulA_LacZ</td><td>This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a SulA Promoter (part BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement |
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Revision as of 10:54, 9 June 2012
| Name | Description | Registry link | Part type |
| precA_LacZ | This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004). | Measurement | |
| psulA_LacZ | This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a SulA Promoter (part BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004). | BBa_K862001 | Measurement |
| precB_LacZ | This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recB Promoter (part BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004). | BBa_K862002 | Measurement |
| precB | The recB promoter sequence was taken for the E. coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.
RecB_fw: aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAACGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc RecB_rev: ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg | BBa_K862003 | Regulatory |
