Team:Heidelberg LSL/Notebook methods

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Revision as of 17:59, 11 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Methods

Transformation

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Inoculation of LB agar plates

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Inoculation of liquid LB medium

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Qiagen Miniprep

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Restriction digest

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SAP digest

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Gel electrophoresis

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Gel extraction

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Qiagen nucleotide removal kit

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Ligation digest

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Oligo annealing

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Colony PCR

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Sequencing

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X-Gal assay

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ONPG assay

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Use of the photometer

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Use of the plate reader

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