Team:Heidelberg LSL/Notebook methods

From 2012hs.igem.org

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<h2>Methods</h2>
<h2>Methods</h2>
<h4>Transformation</h4>
<h4>Transformation</h4>
-
 
<p>...<br/>
<p>...<br/>
-
 
<h4>Inoculation of LB agar plates</h4>
<h4>Inoculation of LB agar plates</h4>
-
...
+
<p>...<br/>
-
 
+
<h4>Inoculation of liquid LB medium </h4>
<h4>Inoculation of liquid LB medium </h4>
-
 
+
<p>...<br/>
<h4>Qiagen Miniprep </h4>
<h4>Qiagen Miniprep </h4>
-
 
+
<p>...<br/>
<h4>Restriction digest </h4>
<h4>Restriction digest </h4>
-
 
+
<p>...<br/>
<h4>SAP digest </h4>
<h4>SAP digest </h4>
-
 
+
<p>...<br/>
<h4>Gel electrophoresis </h4>
<h4>Gel electrophoresis </h4>
-
 
+
<p>...<br/>
<h4>Gel extraction </h4>
<h4>Gel extraction </h4>
-
 
+
<p>...<br/>
<h4>Qiagen nucleotide removal kit </h4>
<h4>Qiagen nucleotide removal kit </h4>
-
 
+
<p>...<br/>
<h4>Ligation digest </h4>
<h4>Ligation digest </h4>
-
 
+
<p>...<br/>
<h4>Oligo annealing </h4>
<h4>Oligo annealing </h4>
-
 
+
<p>...<br/>
<h4>Colony PCR</h4>
<h4>Colony PCR</h4>
-
 
+
<p>...<br/>
<h4>Sequencing </h4>
<h4>Sequencing </h4>
-
 
+
<p>...<br/>
<h4>X-Gal assay </h4>
<h4>X-Gal assay </h4>
-
 
+
<p>...<br/>
<h4>ONPG assay </h4>
<h4>ONPG assay </h4>
-
 
+
<p>...<br/>
<h4>Use of the photometer </h4>
<h4>Use of the photometer </h4>
-
 
+
<p>...<br/>
<h4>Use of the plate reader </h4>
<h4>Use of the plate reader </h4>
-
 
+
<p>...<br/>
<p>&nbsp;</p>
<p>&nbsp;</p>
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Revision as of 12:58, 10 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Methods

Transformation

...

Inoculation of LB agar plates

...

Inoculation of liquid LB medium

...

Qiagen Miniprep

...

Restriction digest

...

SAP digest

...

Gel electrophoresis

...

Gel extraction

...

Qiagen nucleotide removal kit

...

Ligation digest

...

Oligo annealing

...

Colony PCR

...

Sequencing

...

X-Gal assay

...

ONPG assay

...

Use of the photometer

...

Use of the plate reader

...