Team:Heidelberg LSL/Notebook methods

From 2012hs.igem.org

(Difference between revisions)
Line 1: Line 1:
{{Top}}
{{Top}}
{{Stylesheet}}
{{Stylesheet}}
 +
 +
<html>
 +
 +
<style type="text/css">
 +
 +
 +
#bodyContent { background-color: transparent; border: none; width: 975px; min-height: 900px;}
 +
 +
<script type="text/javascript">
 +
function setPageSize() {
 +
len = document.getElementById('super_main_wrapper').offsetHeight;
 +
document.getElementById('bodyContent').style.height = len + 'px';
 +
document.getElementById('news').style.height = len + 'px';
 +
}
 +
</script>
 +
</style>
 +
 +
<body id="sponsors" onload="setPageSize()">
 +
<div id="super_main_wrapper">
 +
<div id="SubWrapper">
 +
 +
<h2>Methods</h2>
 +
<h4>Transformation</h4>
 +
 +
<p>...<br/>
 +
 +
<h4>Inoculation of LB agar plates</h4>
 +
...
 +
 +
<h4>Inoculation of liquid LB medium </h4>
 +
 +
<h4>Qiagen Miniprep </h4>
 +
 +
<h4>Restriction digest </h4>
 +
 +
<h4>SAP digest </h4>
 +
 +
<h4>Gel electrophoresis </h4>
 +
 +
<h4>Gel extraction </h4>
 +
 +
<h4>Qiagen nucleotide removal kit </h4>
 +
 +
<h4>Ligation digest </h4>
 +
 +
<h4>Oligo annealing </h4>
 +
 +
<h4>Colony PCR</h4>
 +
 +
<h4>Sequencing </h4>
 +
 +
<h4>X-Gal assay </h4>
 +
 +
<h4>ONPG assay </h4>
 +
 +
<h4>Use of the photometer </h4>
 +
 +
<h4>Use of the plate reader </h4>
 +
 +
<p>&nbsp;</p>
 +
</div><!--end SubWrapper-->
 +
</div> <!-- end super_main_wrapper>
 +
 +
 +
</body>
 +
 +
 +
</html>

Revision as of 12:57, 10 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Methods

Transformation

...

Inoculation of LB agar plates

...

Inoculation of liquid LB medium

Qiagen Miniprep

Restriction digest

SAP digest

Gel electrophoresis

Gel extraction

Qiagen nucleotide removal kit

Ligation digest

Oligo annealing

Colony PCR

Sequencing

X-Gal assay

ONPG assay

Use of the photometer

Use of the plate reader