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Procedures

PCR Extraction

Add the following to a fresh PCR tube:

1. 2.5 uL 5' Primer

2. 2.5 uL 3' Primer

3. 5 uL Thermopol Buffer

4. 1 uL dNTPs

5. 33.5 ddH2O

6. .5 uL DNA Polymerase

Gel Elctrophoresis

1. Add the following to an Eppindorf tube:

a. 5 uL of the extracted PCR part

b. 15 uL of distilled water

c. 5 uL of PV92 Xylene Cyanole Loading Dye

2. Using a micro pipette, dispense the mixture into a well of the gel.

3. Run the gel until the bands of DNA move to the middle of the gel.

4. Stop the gel and compare the band to the DNA ladder, to find if they are the right length.

5. If it is the right length, extract using filter paper and a scalpel.