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Team:GreenfieldCentral IN/The Project
From 2012hs.igem.org
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!align="center"|[https://2012hs.igem.org/Team:GreenfieldCentral_IN/Notebook Notebook] | !align="center"|[https://2012hs.igem.org/Team:GreenfieldCentral_IN/Notebook Notebook] | ||
!align="center"|[https://2012hs.igem.org/Team:GreenfieldCentral_IN/Safety Safety] | !align="center"|[https://2012hs.igem.org/Team:GreenfieldCentral_IN/Safety Safety] | ||
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Revision as of 04:36, 16 June 2012
| Home | The Team | The Project | Results | Modeling | Human Practices | Notebook | Safety | Sponsors |
|---|
The Project
Procedures
PCR Extraction
Add the following to a fresh PCR tube:
1. 2.5 uL 5' Primer
2. 2.5 uL 3' Primer
3. 5 uL Thermopol Buffer
4. 1 uL dNTPs
5. 33.5 ddH2O
6. .5 uL DNA Polymerase
Gel Elctrophoresis
1. Add the following to an Eppindorf tube:
a. 5 uL of the extracted PCR part
b. 15 uL of distilled water
c. 5 uL of PV92 Xylene Cyanole Loading Dye
2. Using a micro pipette, dispense the mixture into a well of the gel.
3. Run the gel until the bands of DNA move to the middle of the gel.
4. Stop the gel and compare the band to the DNA ladder, to find if they are the right length.
5. If it is the right length, extract using filter paper and a scalpel.
