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From 2012hs.igem.org

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	Team GPHS_Snohomish_WA

Official Team Profile

Contents 1 Arsenic Avengers 2 Project Introduction 3 Notebook 4 Results/Conclusions 5 Safety 6 Attributions 7 Human Practices 8 Fun!

Arsenic Avengers

• Our team is located at Glacier Peak High School in Snohomish, WA.
• We have eight members.
• Our mentors are Eric Lagally, Ms. Caraballo,and Nick Chavkin (UW bioengineering).

Project Introduction

This semester was focused on introducing ourselves to the competition of iGEM, as well as determining our project. The Grizzles have come to the conclusion to utilize the genes in E. coli that detect and flush out arsenic to determine levels of the element in the waters of Puget Sound. If arsenic is present, we have planned to insert a reporter gene that, when synthesized, will turn the E. coli dark green. To make this vision a reality, we are using an arsR promoter, a dark green E. chromi reporter from the 2009 Cambridge team's project. Link to video about Arsenic in Apple Juice File:Map.gif

Notebook

Show us how you spent your days.

We attempted to transform E. Coli with test plasmids provided in our kit. Protocol:

1 Make sure set-up is complete

2 Add 2000mL Cacl2 Solution to each tube.

3 "Harvest NEBlO cells: Scrape off the plate for eact tube Resuspend all the tubes

4 Add DNA to each tube 5mL of "RFP Control" to control tube 2-5mL of other plasmids to labeled tubes

5 Incubate on ice for 5 min.

6 Place in water bath for 90 seconds

7 Place tubes on ice for another minute

8 Add 200mL of transformed cells to plate and spread evenly

9 Label 3 plates, with appropiate drug

10 Add 200mL of transformed cells to plate, spread evenly

11Put plates in incubator

This first trial did not have our desired results

Results/Conclusions

What did you achieve over the course of your semester?

Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?

Attributions

Who worked on what?

Human Practices

What impact does/will your project have on the public?

Fun!

What was your favorite team snack?? Have a picture of your team mascot?

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