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Team:CIDEB-UANL Mexico/Wet-lab/Planning
From 2012hs.igem.org
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<p>To work in the laboratory we made a plan about how to build the circuit. The principal processes are cut and ligate. To cut the biobricks we need restriction enzymes type II. These enzymes cut in a specific place. Here we hace an example about how to cut and ligate the pieces 1-6M and 3-12M:</p> | <p>To work in the laboratory we made a plan about how to build the circuit. The principal processes are cut and ligate. To cut the biobricks we need restriction enzymes type II. These enzymes cut in a specific place. Here we hace an example about how to cut and ligate the pieces 1-6M and 3-12M:</p> | ||
| + | <p>The part 1-6M is cut with the EcoRI and SpeI enzymes</p> | ||
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| + | <center> | ||
| + | <p><a href="http://openwetware.org/wiki/Image:3AAssembly.gif">http://openwetware.org/wiki/Image:3AAssembly.gif</a></p> | ||
| + | </center> | ||
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| + | <p><a href="http://openwetware.org/wiki/Image:3AAssembly.gif">http://openwetware.org/wiki/Image:3AAssembly.gif</a></p> | ||
| + | </center> | ||
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| + | <center> | ||
| + | <div class = "img-holder" style="width:140px; font-size: 18px;"> | ||
| + | <a href="https://static.igem.org/mediawiki/2012hs/e/e3/Blue001c2.png" rel="lightbox"> | ||
| + | <img src="https://static.igem.org/mediawiki/2012hs/e/e3/Blue001c2.png" width="140px" height="170px" align="center"> | ||
| + | </a> | ||
| + | <span class="img-holder-text"><b></b> </div><div class="br"> | ||
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Revision as of 23:36, 16 June 2012
Wet-lab: Planning
To work in the laboratory we made a plan about how to build the circuit. The principal processes are cut and ligate. To cut the biobricks we need restriction enzymes type II. These enzymes cut in a specific place. Here we hace an example about how to cut and ligate the pieces 1-6M and 3-12M:
The part 1-6M is cut with the EcoRI and SpeI enzymes
