Team:AUC Turkey/Notebook

February 25 Saturday

• We learned to make LB Broth.

March 5 Monday

Brainstorming

Ideas

• Geobacteria(Electricity producing bacteria)
• Melanin(Sound absorbing bacteria)
• Salt precipitating bacteria
• Radioactivity absorbing bacteria

March 9 Friday

• We learned how to isolate.

March 10 Saturday

• We isolated Red Fluorescent Protein.

March 12 Monday

Brainstorming

• Cure for dandruff disease
• Bad smell removal
• Heat stabilizing bacteria
• Firefighter bacteria
• Paper cleaner bacteria

March 19 Monday

We decided on what to do for our project.

Final Ideas

• Bad Smell Removal
• Insulin Production

Winner: Bad Smell Removal

March 22 Thursday

• We celebrated the birthday of one of our dear advisors: Mrs. Gunduz
• The research done was analyzed.

March 24 Saturday

• We learned the method of electrophoresis with the RFP`s we previously isolated.
• We learned to do digestion.
• We learned to do transformation.

March 31 Saturday

• We learned to do ligation.

April 2 Monday

We decided on how the smell removing system will work.

Ideas

1)A system consisting of 2 bacterias with 1 producing bad smell and the other producing good smell. 1 of these will give put bad smell and the other will get rid of this smell and turn into something good and also inhibate the other one.

2)A system in which a bacteria removes bad smell and uses the products to produce good smell. The bacteria will use receptors to detect the products of the removal process and use them to give out good smell.

April 5 Thursday

• We discussed the ideas from last saturday. We weren`t able to find a conclusion.

April 7 Saturday

• We learned to do gel extraction.

April 9 Monday

• We researched for genes and enzymes which are compatible with our project.
• We took a team photo.

April 21 Saturday

• We transformated the parts E0022 and E0032.

April 26 Thursday

• We discussed what we can do for human practice.

April 28 Saturday

• We isolated the parts E0022 and E0023. We diluted the parts C0061, C0062, R0062, R0063, K118024, K118025, J45120, J45200, J45320, J09855, J23100, J52034 from the kit iGEM sent us.

May 2 Wednesday

• We transformated the parts C0061, C0062, R0062, R0063, K118024, K118025, J45120, J45200, J45320, J09855, J23100, J52034.

May 9 Wednesday

• When we came to the lab, we saw that the transformations from last week were not successful. We transformated the same parts again.

May 11 Friday

• Our arrival to the lab revealed that we couldn't successfully transformate for the second time. This result led us to find our mistake. We did several things to locate our mistake. Now we had nothing to do but wait for a few days to see what's wrong.

May 15 Tuesday

• The results of our experiments showed that the compotent cells that we have used were not okay. We repeated the process to ensure that we really figured out what the problem is. Yes,the problem was the compotent cells. The only problem was that we needed to make new compotent cells. Since this process takes time and our lack of experience on making compotent cells is huge,this meant great time loss for us.

May 18 Friday

• We were finally able to succeed in transforming our parts with the new compotent cells.

May 21 Monday

• We made LB media with the colonies taken from our plates. Since there was a 16 hour wait time, we had no choice but to wait.

May 22 Tuesday

• We isolated our LB media. When we went to spectrophotometer for the results, it was embrassing. Our plasmids had a concentration of approximately 2 ng/ml. We made another patch of LB media to isolate again.

May 26 Saturday

• We had the same horrible results with the isolation. Again, LB media preparation...

May 27 Sunday

• Same awesome results were acquired with the isolation. We repeated the process again.

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