http://2012hs.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=500&target=Dniopek&year=&month=2012hs.igem.org - User contributions [en]2024-03-29T11:09:53ZFrom 2012hs.igem.orgMediaWiki 1.16.0http://2012hs.igem.org/File:Measurement_GFP_HD2012.pngFile:Measurement GFP HD2012.png2012-07-18T10:43:53Z<p>Dniopek: uploaded a new version of &quot;File:Measurement GFP HD2012.png&quot;</p>
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<div></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/MeasurementTeam:Heidelberg LSL/Measurement2012-07-18T10:43:14Z<p>Dniopek: </p>
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<h2>Measurement</h2><br />
<br />
<a name="background"><h4>Background</h4></a><br />
<p><br />
For realizing our vision to construct bacteria that detect UV and radioactive radiation and give a color output visible by eye, we <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project_UVsensors">constructed three different sensor parts</a> based on the <i>E. coli</i> <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project_SOS">SOS-response promoters</a>. These are the promoters precA, precB and psulA combined with a LacZ reporter. <i>E.coli</i> bacteria transformed with our sensors show a highly sensitive, UV-dose dependent coloring, as measured by both ONPG (figure 1) and X-Gal assays (figure 2). We show that our promoters also work in combination with other reporters such as GFP (figure 3), proofing the modularity of the approach we propose.<br/><br />
Finally, we proof the performence of two submitted parts (precA-LacZ, part <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a> and psulA-LacZ, part <a href="http://partsregistry.org/Part:BBa_K862001">BBa_K862001</a>) in an outdoor experiment, where we really induced our bacteria in the summer sun (figure 4). <br />
Taken together, we show that our standardized DNA-damage sensors provide an easy and highly sensitive approach for the detection of UV-radiation, which can in principle be extended to other radiation sources, such as radioactive radiation and could even be used for the detection of DNA damaging agencies in the future.<br />
</p><br />
<a name="measurement"><h4>Measurements principle – our part characterization</h4></a><br />
<p><br />
We transformed our UV radiation measurement constructs, namely parts <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a>, <a href="http://partsregistry.org/Part:BBa_K862001">BBa_K862001</a> and <a href="http://partsregistry.org/Part:BBa_K862002">BBa_K862002</a> into BL21(DE3) bacteria (recA+ strain). The basic measurement strategy we used was illumination of bactrial o/n cultures in 6- or 24- well plate formats in a UV geldoc chamber for different time periods. We primarily used beta galactosidase (LacZ) as a reporter and added to substrate (ONPG giving a yellow color with a max. absorbance at 420 nm or X-Gal giving a blue color with a max. absorbance at 610 nm) after illumination of the samples. Color development was quantified using a plate reader (ONPG assay) or by calculating the color intensities from RGB pictures taken with a digital camera using ImageJ (X-Gal assay). For our GFP reporter test, bright-field fluorescence microscopy was applied and GFP expression was quantified from pictures taken from UV-irradiated versus non-irradiated bacteria.<br />
</p><br />
<br />
<br />
<a name="ONPG"><h4>1) ONPG assay for the sensitive measurement of reporter activity already after very short UV exposure times</h4></a><br />
<img src="https://static.igem.org/mediawiki/2012hs/e/e7/ONPG_schema.png" width="635"/><br />
<br/><br/><br />
<br />
<p>In order to determine the response of our sulA and recA promoters to low UV doses, we used the highly sensitive ONPG assay.<br/><br />
ONPG (Ortho-nitrophenyl-β-D-galactopyranoside) is a synthetic lacZ substrate and produces a yellow color (o-nitrophenol) upon cleavage by LacZ. The formation of the yellow color can be easily determined using a photometer at 420 nm absorbance wavelength. 0.5 ml of the overnight culture of Bl21(DE3) transformed with our precA_lacZ or psulA_acZ constructs were put into each well of a 6-well plate. <br />
Cells were irradiated in a Geldoc-UV-Chamber for 0-600 s. 10 min after irradiation, an ONPG assay was performed in a 96-well format (using technical duplicates).<br/><br />
</p><center><img src="https://static.igem.org/mediawiki/2012hs/8/80/Bildschirmfoto_2012-06-11_um_23.47.05.png" width="500"/></center><br />
<p><br />
<b>Fig. 1: ONPG assay of Bl21(DE3) irradiated for different times. </b>Both constructs show a strong correlation between the UV-irradiation time and the LacZ activity (production of o-nitrophenol). <br/><br />
<br/><br />
Both, the psulA_LacZ and the precA_LacZ constructs, show a nice increase of LacZ activity with increasing UV irradiation times already 10 min after induction. This argues for a rapid response by our sensor constructs which is due to the overall rapid SOS response initiated by the UV radiation. The OD did not change with UV radiation, showing that the cell number was not effected by the UV treatment.</p><br />
<br />
<br />
<a name="XGAL"><h4>2) X-Gal Assay for the characterization of our parts in an application-relevant context</h4><br />
<img src="https://static.igem.org/mediawiki/2012hs/e/eb/Xgal_schema.png" width="635"/></a><br />
<br/><br />
<br/><br />
<br />
<p>For chracterizing the precA, precB and psulA constructs with lacZ reporter (partsregistry: <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a>, <a href="http://partsregistry.org/Part:BBa_K862002">BBa_K862002</a> and <a href="http://partsregistry.org/Part:BBa_K862001">BBa_K862001</a>)in a close-to application context, we performed X-Gal assays. Therefore, we put o/n cultures of <i>E.coli</i> transformed with our sensor parts onto 24 well plates (12 of the same culture samples, 0.6 ml/well, each construct onto a separate plate). We induced the plates for 0 s and transfered the first 2 samples onto a several plate. After 5 min induction we put the second two samples onto a serparate plate and so on. Thereby, we subsequently induced our samples and got induction times between 0 and 30 min with 2 replicates for each induction time. The start point of induction is the same for all samples. After 1 h incubation at 37 °C/80 rpm we added X-Gal substrate (final concentration of 200 µg/ml) to all samples. Coloring of the wells were monitored by taking pictures 15 min after X-gal addition (figure 2, left). Quantifications of the coloring intensity were done from the pictures taken by pixel grey-value analysis in ImageJ in the different wells (figure 2, right).<br />
</p><br />
<center><img src="https://static.igem.org/mediawiki/2012hs/c/c7/XGAL_Measurement.PNG" width="635"/></center><br />
<p><br />
<b>Fig. 2: X-gal assay of Bl21(DE3) transformed with precA/precB/psulA_LacZ parts and irradiated for different times. </b>All constructs show a strong positive correlation between UV induction time and coloring of the wells. PrecA-LacZ gives the lowest reporter background expression whereas psulA-LacZ gives the highest overall coloring of the samples.<br />
<br />
All constructs show a clear, UV dose-dependent coloring, that is visible already by eye, showing that the constructs are working really great. Whereas the precA construct has the lowest promoter background activity (almost no coloring at 0 min time point), the psulA shows the largest color development for high UV doses (20 and 30 min). Therefore, all promoters have slightly different properties and would have certain advantages or disadvantages in different application contexts (i.e. in short or long-term UV measurements).</p><br />
<br />
<br />
<a name="GFP"><h4>3) Test of alternative reporter: GFP fluorescence induction using our precA-GFP construct</h4></a><br />
<img src="https://static.igem.org/mediawiki/2012hs/3/38/RecA_GFP_microscopy_HD2012.png" width="635"/><br/><br />
<br/><br />
<p>For investigating, whether our approach is also working for other reporters than lacZ, we tested our precA-GFP construct under similar conditions. An overnight culture of <i>E. coli</i> BL21(DE3) transformed with precA-GFP (see construct #1 in <a href= "https://2012hs.igem.org/Team:Heidelberg_LSL/Project_UVsensors" > the sensor construction page </a> was distributed onto 2 6-well plates (3 ml/well, experiment done in duplicates) and either induced by UV-irradiation for 30 min or left uninduced. 30 min after induction fluorescence microscopy was performed (excitation at 470 nm, GFP emission filter). GFP expression was quantified from the microscope pictures using ImageJ.<br/><br />
</p><br />
<center><img src="https://static.igem.org/mediawiki/2012hs/0/03/Measurement_GFP_HD2012.png" width="500"/></center><br />
<p><br />
<b>Fig. 3: Test of precA-GFP reporter construct by fluorescence microscopy. </b><i>E.coli</i> transformed with our precA-GFP reporter were either UV-irradiated for 30 min or left uninduced. measurement of GFP expression show a 10-fold (!) increase in GFP expression due to UV-irradiation compared to the control. <br/><br />
<br/><br />
<i>E. coli</i> transformed with our precA-GFP measurement constructs show a 10 fold (!) induction of GFP expression after 30 min of UV irradiation compared to the non-irradiated control. This proves that our modular approach of combining endogenous SOS-promoters such as precA, precB and psulA with different reporters works really fine. Therefor, we give users of our constructs the opportunity to measure radiation by using different measurement setups (X-gal assay, fluorescence microscopy and potentially many more). </p><br />
<br />
<a name="Outdoor"><h4>4) Outdoor-Test und real-life application conditions</h4></a><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/04/Outdoor_schema.png" width="635"/><br/><br />
<br/><br />
<p>Finally, in order to test our UV radiation sensor construct in a real application setting, we went outside and used the rarely shining German sun as UV irradiation device. First, o/n cultures with <i>E. coli</i> transformed with either precA-LacZ or psulA-LacZ were put into two different 6-well plates (3 ml/well). The 6-well plates were tightly sealed, glued and additionally wrapped with parafilm. Afterwards, the plate covers were thoroughly desinfected using 70 % ethanol and taken outside. The plates were placed either in the bright sun (it was an average early-summer day) or in the shade. Afterwards, the plates were taken back to the lab and incubated for 30 min at room temperature in the dark. Finally, 2 ml of each of the 4 samples (precA_LacZ and psulA_LacZ, each either incubated in the sun or in the shadow) were pipetted into a cuvette and X-gal was added to a final concentration of 200 µg/ml. Then pictures were subsequently taken every minute while the blue color was developing (we started taking the pictures 2 min after X-gal addition).</p><br />
<br />
<img src="https://static.igem.org/mediawiki/2012hs/f/f9/Outdoor-Test.png" width="635"><br />
<br />
<p><br />
<b>Fig. 4: real application outdoor test of precA_LacZ and psulA_LacZ in the summer sun. </b>In order to make a final evaluation of our UV radiation sensor approach, we incubated bacteria transformed with our precA_LacZ and psulA_LacZ in the summer sun for 70 min or in the shade as negative control. Afterwards, we put our bacteria into cuvettes, added X-gal and monitored the development of the blue color over time. Obviously, both constructs allowed a clear distinction between the bacteria incubated in the sun and in the shade. The precA_LacZ construct performed better than the psulA_LacZ, as the precA_LacZ-Promoter had almost no background activity when bacteria were incubated in the shade. <br/><br />
</p><br />
<a name="Wrapup"><h4>Wrap-up</h4></a><br />
<p>Our analysis clearly proof that our radiation sensor constructs are highly sensitive, even to low UV irradiation doses (see fig. 1, ONPG-assay), show a clear, broad UV-dose dependent activation dynamics (see fig. 2, X-gal assay) and are even working in a real application setup (see fig. 4, outdoor test). By using a LacZ reporter gene, we enable a very simple measurement approach, where to user can simply monitor the blue color development by eye without the need for any complicated or expensive measurement equipment.<br/><br />
In addition, the approach we propose here is highly modular, so that our promoters, namely precA, precB and psulA could be combined with all different kinds of reporters, which we exemplified by using both LacZ and GFP as reporter (see fig. 3, precA-GFP test).</p><br />
<br />
<p>&nbsp;</p><br />
</div><!--end SubWrapper--><br />
<div id="news"><br />
<center><h4>Content</h4></center><br />
<ul style="margin-left:30px;"><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#background">Background</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#measurement">Measurements Principle</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#ONPG">1) ONPG-Assay</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#XGAL">2) X-Gal-Assay</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#GFP">3) GFP-Reporter Test</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#Outdoor">Outdoor Test</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#Wrapup">Wrap-up</a></li><br />
</ul><br />
</div><!--end news--><br />
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</html></div>Dniopekhttp://2012hs.igem.org/File:Measurement_GFP_HD2012.pngFile:Measurement GFP HD2012.png2012-07-18T10:42:44Z<p>Dniopek: </p>
<hr />
<div></div>Dniopekhttp://2012hs.igem.org/File:RecA_GFP_microscopy_HD2012.pngFile:RecA GFP microscopy HD2012.png2012-07-18T10:42:33Z<p>Dniopek: uploaded a new version of &quot;File:RecA GFP microscopy HD2012.png&quot;</p>
<hr />
<div></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/MeasurementTeam:Heidelberg LSL/Measurement2012-07-18T10:41:11Z<p>Dniopek: </p>
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<h2>Measurement</h2><br />
<br />
<a name="background"><h4>Background</h4></a><br />
<p><br />
For realizing our vision to construct bacteria that detect UV and radioactive radiation and give a color output visible by eye, we <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project_UVsensors">constructed three different sensor parts</a> based on the <i>E. coli</i> <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project_SOS">SOS-response promoters</a>. These are the promoters precA, precB and psulA combined with a LacZ reporter. <i>E.coli</i> bacteria transformed with our sensors show a highly sensitive, UV-dose dependent coloring, as measured by both ONPG (figure 1) and X-Gal assays (figure 2). We show that our promoters also work in combination with other reporters such as GFP (figure 3), proofing the modularity of the approach we propose.<br/><br />
Finally, we proof the performence of two submitted parts (precA-LacZ, part <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a> and psulA-LacZ, part <a href="http://partsregistry.org/Part:BBa_K862001">BBa_K862001</a>) in an outdoor experiment, where we really induced our bacteria in the summer sun (figure 4). <br />
Taken together, we show that our standardized DNA-damage sensors provide an easy and highly sensitive approach for the detection of UV-radiation, which can in principle be extended to other radiation sources, such as radioactive radiation and could even be used for the detection of DNA damaging agencies in the future.<br />
</p><br />
<a name="measurement"><h4>Measurements principle – our part characterization</h4></a><br />
<p><br />
We transformed our UV radiation measurement constructs, namely parts <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a>, <a href="http://partsregistry.org/Part:BBa_K862001">BBa_K862001</a> and <a href="http://partsregistry.org/Part:BBa_K862002">BBa_K862002</a> into BL21(DE3) bacteria (recA+ strain). The basic measurement strategy we used was illumination of bactrial o/n cultures in 6- or 24- well plate formats in a UV geldoc chamber for different time periods. We primarily used beta galactosidase (LacZ) as a reporter and added to substrate (ONPG giving a yellow color with a max. absorbance at 420 nm or X-Gal giving a blue color with a max. absorbance at 610 nm) after illumination of the samples. Color development was quantified using a plate reader (ONPG assay) or by calculating the color intensities from RGB pictures taken with a digital camera using ImageJ (X-Gal assay). For our GFP reporter test, bright-field fluorescence microscopy was applied and GFP expression was quantified from pictures taken from UV-irradiated versus non-irradiated bacteria.<br />
</p><br />
<br />
<br />
<a name="ONPG"><h4>1) ONPG assay for the sensitive measurement of reporter activity already after very short UV exposure times</h4></a><br />
<img src="https://static.igem.org/mediawiki/2012hs/e/e7/ONPG_schema.png" width="635"/><br />
<br/><br/><br />
<br />
<p>In order to determine the response of our sulA and recA promoters to low UV doses, we used the highly sensitive ONPG assay.<br/><br />
ONPG (Ortho-nitrophenyl-β-D-galactopyranoside) is a synthetic lacZ substrate and produces a yellow color (o-nitrophenol) upon cleavage by LacZ. The formation of the yellow color can be easily determined using a photometer at 420 nm absorbance wavelength. 0.5 ml of the overnight culture of Bl21(DE3) transformed with our precA_lacZ or psulA_acZ constructs were put into each well of a 6-well plate. <br />
Cells were irradiated in a Geldoc-UV-Chamber for 0-600 s. 10 min after irradiation, an ONPG assay was performed in a 96-well format (using technical duplicates).<br/><br />
</p><center><img src="https://static.igem.org/mediawiki/2012hs/8/80/Bildschirmfoto_2012-06-11_um_23.47.05.png" width="500"/></center><br />
<p><br />
<b>Fig. 1: ONPG assay of Bl21(DE3) irradiated for different times. </b>Both constructs show a strong correlation between the UV-irradiation time and the LacZ activity (production of o-nitrophenol). <br/><br />
<br/><br />
Both, the psulA_LacZ and the precA_LacZ constructs, show a nice increase of LacZ activity with increasing UV irradiation times already 10 min after induction. This argues for a rapid response by our sensor constructs which is due to the overall rapid SOS response initiated by the UV radiation. The OD did not change with UV radiation, showing that the cell number was not effected by the UV treatment.</p><br />
<br />
<br />
<a name="XGAL"><h4>2) X-Gal Assay for the characterization of our parts in an application-relevant context</h4><br />
<img src="https://static.igem.org/mediawiki/2012hs/e/eb/Xgal_schema.png" width="635"/></a><br />
<br/><br />
<br/><br />
<br />
<p>For chracterizing the precA, precB and psulA constructs with lacZ reporter (partsregistry: <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a>, <a href="http://partsregistry.org/Part:BBa_K862002">BBa_K862002</a> and <a href="http://partsregistry.org/Part:BBa_K862001">BBa_K862001</a>)in a close-to application context, we performed X-Gal assays. Therefore, we put o/n cultures of <i>E.coli</i> transformed with our sensor parts onto 24 well plates (12 of the same culture samples, 0.6 ml/well, each construct onto a separate plate). We induced the plates for 0 s and transfered the first 2 samples onto a several plate. After 5 min induction we put the second two samples onto a serparate plate and so on. Thereby, we subsequently induced our samples and got induction times between 0 and 30 min with 2 replicates for each induction time. The start point of induction is the same for all samples. After 1 h incubation at 37 °C/80 rpm we added X-Gal substrate (final concentration of 200 µg/ml) to all samples. Coloring of the wells were monitored by taking pictures 15 min after X-gal addition (figure 2, left). Quantifications of the coloring intensity were done from the pictures taken by pixel grey-value analysis in ImageJ in the different wells (figure 2, right).<br />
</p><br />
<center><img src="https://static.igem.org/mediawiki/2012hs/c/c7/XGAL_Measurement.PNG" width="635"/></center><br />
<p><br />
<b>Fig. 2: X-gal assay of Bl21(DE3) transformed with precA/precB/psulA_LacZ parts and irradiated for different times. </b>All constructs show a strong positive correlation between UV induction time and coloring of the wells. PrecA-LacZ gives the lowest reporter background expression whereas psulA-LacZ gives the highest overall coloring of the samples.<br />
<br />
All constructs show a clear, UV dose-dependent coloring, that is visible already by eye, showing that the constructs are working really great. Whereas the precA construct has the lowest promoter background activity (almost no coloring at 0 min time point), the psulA shows the largest color development for high UV doses (20 and 30 min). Therefore, all promoters have slightly different properties and would have certain advantages or disadvantages in different application contexts (i.e. in short or long-term UV measurements).</p><br />
<br />
<br />
<a name="GFP"><h4>3) Test of alternative reporter: GFP fluorescence induction using our precA-GFP construct</h4></a><br />
<img src="https://static.igem.org/mediawiki/2012hs/3/38/RecA_GFP_microscopy_HD2012.png" width="635"/><br/><br />
<br/><br />
<p>For investigating, whether our approach is also working for other reporters than lacZ, we tested our precA-GFP construct under similar conditions. An overnight culture of <i>E. coli</i> BL21(DE3) transformed with precA-GFP (see construct #1 in <a href= "https://2012hs.igem.org/Team:Heidelberg_LSL/Project_UVsensors" > the sensor construction page </a> was distributed onto 2 6-well plates (3 ml/well, experiment done in duplicates) and either induced by UV-irradiation for 30 min or left uninduced. 30 min after induction fluorescence microscopy was performed (excitation at 470 nm, GFP emission filter). GFP expression was quantified from the microscope pictures using ImageJ.<br/><br />
</p><br />
<center><img src="https://static.igem.org/mediawiki/2012hs/3/38/GFP_Measurement.png" width="500"/></center><br />
<p><br />
<b>Fig. 3: Test of precA-GFP reporter construct by fluorescence microscopy. </b><i>E.coli</i> transformed with our precA-GFP reporter were either UV-irradiated for 30 min or left uninduced. measurement of GFP expression show a 10-fold (!) increase in GFP expression due to UV-irradiation compared to the control. <br/><br />
<br/><br />
<i>E. coli</i> transformed with our precA-GFP measurement constructs show a 10 fold (!) induction of GFP expression after 30 min of UV irradiation compared to the non-irradiated control. This proves that our modular approach of combining endogenous SOS-promoters such as precA, precB and psulA with different reporters works really fine. Therefor, we give users of our constructs the opportunity to measure radiation by using different measurement setups (X-gal assay, fluorescence microscopy and potentially many more). </p><br />
<br />
<a name="Outdoor"><h4>4) Outdoor-Test und real-life application conditions</h4></a><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/04/Outdoor_schema.png" width="635"/><br/><br />
<br/><br />
<p>Finally, in order to test our UV radiation sensor construct in a real application setting, we went outside and used the rarely shining German sun as UV irradiation device. First, o/n cultures with <i>E. coli</i> transformed with either precA-LacZ or psulA-LacZ were put into two different 6-well plates (3 ml/well). The 6-well plates were tightly sealed, glued and additionally wrapped with parafilm. Afterwards, the plate covers were thoroughly desinfected using 70 % ethanol and taken outside. The plates were placed either in the bright sun (it was an average early-summer day) or in the shade. Afterwards, the plates were taken back to the lab and incubated for 30 min at room temperature in the dark. Finally, 2 ml of each of the 4 samples (precA_LacZ and psulA_LacZ, each either incubated in the sun or in the shadow) were pipetted into a cuvette and X-gal was added to a final concentration of 200 µg/ml. Then pictures were subsequently taken every minute while the blue color was developing (we started taking the pictures 2 min after X-gal addition).</p><br />
<br />
<img src="https://static.igem.org/mediawiki/2012hs/f/f9/Outdoor-Test.png" width="635"><br />
<br />
<p><br />
<b>Fig. 4: real application outdoor test of precA_LacZ and psulA_LacZ in the summer sun. </b>In order to make a final evaluation of our UV radiation sensor approach, we incubated bacteria transformed with our precA_LacZ and psulA_LacZ in the summer sun for 70 min or in the shade as negative control. Afterwards, we put our bacteria into cuvettes, added X-gal and monitored the development of the blue color over time. Obviously, both constructs allowed a clear distinction between the bacteria incubated in the sun and in the shade. The precA_LacZ construct performed better than the psulA_LacZ, as the precA_LacZ-Promoter had almost no background activity when bacteria were incubated in the shade. <br/><br />
</p><br />
<a name="Wrapup"><h4>Wrap-up</h4></a><br />
<p>Our analysis clearly proof that our radiation sensor constructs are highly sensitive, even to low UV irradiation doses (see fig. 1, ONPG-assay), show a clear, broad UV-dose dependent activation dynamics (see fig. 2, X-gal assay) and are even working in a real application setup (see fig. 4, outdoor test). By using a LacZ reporter gene, we enable a very simple measurement approach, where to user can simply monitor the blue color development by eye without the need for any complicated or expensive measurement equipment.<br/><br />
In addition, the approach we propose here is highly modular, so that our promoters, namely precA, precB and psulA could be combined with all different kinds of reporters, which we exemplified by using both LacZ and GFP as reporter (see fig. 3, precA-GFP test).</p><br />
<br />
<p>&nbsp;</p><br />
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<div id="news"><br />
<center><h4>Content</h4></center><br />
<ul style="margin-left:30px;"><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#background">Background</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#measurement">Measurements Principle</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#ONPG">1) ONPG-Assay</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#XGAL">2) X-Gal-Assay</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#GFP">3) GFP-Reporter Test</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#Outdoor">Outdoor Test</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#Wrapup">Wrap-up</a></li><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/MeasurementTeam:Heidelberg LSL/Measurement2012-07-18T10:40:04Z<p>Dniopek: </p>
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<h2>Measurement</h2><br />
<br />
<a name="background"><h4>Background</h4></a><br />
<p><br />
For realizing our vision to construct bacteria that detect UV and radioactive radiation and give a color output visible by eye, we <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project_UVsensors">constructed three different sensor parts</a> based on the <i>E. coli</i> <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project_SOS">SOS-response promoters</a>. These are the promoters precA, precB and psulA combined with a LacZ reporter. <i>E.coli</i> bacteria transformed with our sensors show a highly sensitive, UV-dose dependent coloring, as measured by both ONPG (figure 1) and X-Gal assays (figure 2). We show that our promoters also work in combination with other reporters such as GFP (figure 3), proofing the modularity of the approach we propose.<br/><br />
Finally, we proof the performence of two submitted parts (precA-LacZ, part <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a> and psulA-LacZ, part <a href="http://partsregistry.org/Part:BBa_K862001">BBa_K862001</a>) in an outdoor experiment, where we really induced our bacteria in the summer sun (figure 4). <br />
Taken together, we show that our standardized DNA-damage sensors provide an easy and highly sensitive approach for the detection of UV-radiation, which can in principle be extended to other radiation sources, such as radioactive radiation and could even be used for the detection of DNA damaging agencies in the future.<br />
</p><br />
<a name="measurement"><h4>Measurements principle – our part characterization</h4></a><br />
<p><br />
We transformed our UV radiation measurement constructs, namely parts <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a>, <a href="http://partsregistry.org/Part:BBa_K862001">BBa_K862001</a> and <a href="http://partsregistry.org/Part:BBa_K862002">BBa_K862002</a> into BL21(DE3) bacteria (recA+ strain). The basic measurement strategy we used was illumination of bactrial o/n cultures in 6- or 24- well plate formats in a UV geldoc chamber for different time periods. We primarily used beta galactosidase (LacZ) as a reporter and added to substrate (ONPG giving a yellow color with a max. absorbance at 420 nm or X-Gal giving a blue color with a max. absorbance at 610 nm) after illumination of the samples. Color development was quantified using a plate reader (ONPG assay) or by calculating the color intensities from RGB pictures taken with a digital camera using ImageJ (X-Gal assay). For our GFP reporter test, bright-field fluorescence microscopy was applied and GFP expression was quantified from pictures taken from UV-irradiated versus non-irradiated bacteria.<br />
</p><br />
<br />
<br />
<a name="ONPG"><h4>1) ONPG assay for the sensitive measurement of reporter activity already after very short UV exposure times</h4></a><br />
<img src="https://static.igem.org/mediawiki/2012hs/e/e7/ONPG_schema.png" width="635"/><br />
<br/><br/><br />
<br />
<p>In order to determine the response of our sulA and recA promoters to low UV doses, we used the highly sensitive ONPG assay.<br/><br />
ONPG (Ortho-nitrophenyl-β-D-galactopyranoside) is a synthetic lacZ substrate and produces a yellow color (o-nitrophenol) upon cleavage by LacZ. The formation of the yellow color can be easily determined using a photometer at 420 nm absorbance wavelength. 0.5 ml of the overnight culture of Bl21(DE3) transformed with our precA_lacZ or psulA_acZ constructs were put into each well of a 6-well plate. <br />
Cells were irradiated in a Geldoc-UV-Chamber for 0-600 s. 10 min after irradiation, an ONPG assay was performed in a 96-well format (using technical duplicates).<br/><br />
</p><center><img src="https://static.igem.org/mediawiki/2012hs/8/80/Bildschirmfoto_2012-06-11_um_23.47.05.png" width="500"/></center><br />
<p><br />
<b>Fig. 1: ONPG assay of Bl21(DE3) irradiated for different times. </b>Both constructs show a strong correlation between the UV-irradiation time and the LacZ activity (production of o-nitrophenol). <br/><br />
<br/><br />
Both, the psulA_LacZ and the precA_LacZ constructs, show a nice increase of LacZ activity with increasing UV irradiation times already 10 min after induction. This argues for a rapid response by our sensor constructs which is due to the overall rapid SOS response initiated by the UV radiation. The OD did not change with UV radiation, showing that the cell number was not effected by the UV treatment.</p><br />
<br />
<br />
<a name="XGAL"><h4>2) X-Gal Assay for the characterization of our parts in an application-relevant context</h4><br />
<img src="https://static.igem.org/mediawiki/2012hs/e/eb/Xgal_schema.png" width="635"/></a><br />
<br/><br />
<br/><br />
<br />
<p>For chracterizing the precA, precB and psulA constructs with lacZ reporter (partsregistry: <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a>, <a href="http://partsregistry.org/Part:BBa_K862002">BBa_K862002</a> and <a href="http://partsregistry.org/Part:BBa_K862001">BBa_K862001</a>)in a close-to application context, we performed X-Gal assays. Therefore, we put o/n cultures of <i>E.coli</i> transformed with our sensor parts onto 24 well plates (12 of the same culture samples, 0.6 ml/well, each construct onto a separate plate). We induced the plates for 0 s and transfered the first 2 samples onto a several plate. After 5 min induction we put the second two samples onto a serparate plate and so on. Thereby, we subsequently induced our samples and got induction times between 0 and 30 min with 2 replicates for each induction time. The start point of induction is the same for all samples. After 1 h incubation at 37 °C/80 rpm we added X-Gal substrate (final concentration of 200 µg/ml) to all samples. Coloring of the wells were monitored by taking pictures 15 min after X-gal addition (figure 2, left). Quantifications of the coloring intensity were done from the pictures taken by pixel grey-value analysis in ImageJ in the different wells (figure 2, right).<br />
</p><br />
<center><img src="https://static.igem.org/mediawiki/2012hs/c/c7/XGAL_Measurement.PNG" width="635"/></center><br />
<p><br />
<b>Fig. 2: X-gal assay of Bl21(DE3) transformed with precA/precB/psulA_LacZ parts and irradiated for different times. </b>All constructs show a strong positive correlation between UV induction time and coloring of the wells. PrecA-LacZ gives the lowest reporter background expression whereas psulA-LacZ gives the highest overall coloring of the samples.<br />
<br />
All constructs show a clear, UV dose-dependent coloring, that is visible already by eye, showing that the constructs are working really great. Whereas the precA construct has the lowest promoter background activity (almost no coloring at 0 min time point), the psulA shows the largest color development for high UV doses (20 and 30 min). Therefore, all promoters have slightly different properties and would have certain advantages or disadvantages in different application contexts (i.e. in short or long-term UV measurements).</p><br />
<br />
<br />
<a name="GFP"><h4>3) Test of alternative reporter: GFP fluorescence induction using our precA-GFP construct</h4></a><br />
<img src="https://static.igem.org/mediawiki/2012hs/4/43/GFP_schema.png" width="635"/><br/><br />
<br/><br />
<p>For investigating, whether our approach is also working for other reporters than lacZ, we tested our precA-GFP construct under similar conditions. An overnight culture of <i>E. coli</i> BL21(DE3) transformed with precA-GFP (see construct #1 in <a href= "https://2012hs.igem.org/Team:Heidelberg_LSL/Project_UVsensors" > the sensor construction page </a> was distributed onto 2 6-well plates (3 ml/well, experiment done in duplicates) and either induced by UV-irradiation for 30 min or left uninduced. 30 min after induction fluorescence microscopy was performed (excitation at 470 nm, GFP emission filter). GFP expression was quantified from the microscope pictures using ImageJ.<br/><br />
</p><br />
<center><img src="https://static.igem.org/mediawiki/2012hs/3/38/RecA_GFP_microscopy_HD2012.png" width="500"/></center><br />
<p><br />
<b>Fig. 3: Test of precA-GFP reporter construct by fluorescence microscopy. </b><i>E.coli</i> transformed with our precA-GFP reporter were either UV-irradiated for 30 min or left uninduced. measurement of GFP expression show a 10-fold (!) increase in GFP expression due to UV-irradiation compared to the control. <br/><br />
<br/><br />
<i>E. coli</i> transformed with our precA-GFP measurement constructs show a 10 fold (!) induction of GFP expression after 30 min of UV irradiation compared to the non-irradiated control. This proves that our modular approach of combining endogenous SOS-promoters such as precA, precB and psulA with different reporters works really fine. Therefor, we give users of our constructs the opportunity to measure radiation by using different measurement setups (X-gal assay, fluorescence microscopy and potentially many more). </p><br />
<br />
<a name="Outdoor"><h4>4) Outdoor-Test und real-life application conditions</h4></a><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/04/Outdoor_schema.png" width="635"/><br/><br />
<br/><br />
<p>Finally, in order to test our UV radiation sensor construct in a real application setting, we went outside and used the rarely shining German sun as UV irradiation device. First, o/n cultures with <i>E. coli</i> transformed with either precA-LacZ or psulA-LacZ were put into two different 6-well plates (3 ml/well). The 6-well plates were tightly sealed, glued and additionally wrapped with parafilm. Afterwards, the plate covers were thoroughly desinfected using 70 % ethanol and taken outside. The plates were placed either in the bright sun (it was an average early-summer day) or in the shade. Afterwards, the plates were taken back to the lab and incubated for 30 min at room temperature in the dark. Finally, 2 ml of each of the 4 samples (precA_LacZ and psulA_LacZ, each either incubated in the sun or in the shadow) were pipetted into a cuvette and X-gal was added to a final concentration of 200 µg/ml. Then pictures were subsequently taken every minute while the blue color was developing (we started taking the pictures 2 min after X-gal addition).</p><br />
<br />
<img src="https://static.igem.org/mediawiki/2012hs/f/f9/Outdoor-Test.png" width="635"><br />
<br />
<p><br />
<b>Fig. 4: real application outdoor test of precA_LacZ and psulA_LacZ in the summer sun. </b>In order to make a final evaluation of our UV radiation sensor approach, we incubated bacteria transformed with our precA_LacZ and psulA_LacZ in the summer sun for 70 min or in the shade as negative control. Afterwards, we put our bacteria into cuvettes, added X-gal and monitored the development of the blue color over time. Obviously, both constructs allowed a clear distinction between the bacteria incubated in the sun and in the shade. The precA_LacZ construct performed better than the psulA_LacZ, as the precA_LacZ-Promoter had almost no background activity when bacteria were incubated in the shade. <br/><br />
</p><br />
<a name="Wrapup"><h4>Wrap-up</h4></a><br />
<p>Our analysis clearly proof that our radiation sensor constructs are highly sensitive, even to low UV irradiation doses (see fig. 1, ONPG-assay), show a clear, broad UV-dose dependent activation dynamics (see fig. 2, X-gal assay) and are even working in a real application setup (see fig. 4, outdoor test). By using a LacZ reporter gene, we enable a very simple measurement approach, where to user can simply monitor the blue color development by eye without the need for any complicated or expensive measurement equipment.<br/><br />
In addition, the approach we propose here is highly modular, so that our promoters, namely precA, precB and psulA could be combined with all different kinds of reporters, which we exemplified by using both LacZ and GFP as reporter (see fig. 3, precA-GFP test).</p><br />
<br />
<p>&nbsp;</p><br />
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<div id="news"><br />
<center><h4>Content</h4></center><br />
<ul style="margin-left:30px;"><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#background">Background</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#measurement">Measurements Principle</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#ONPG">1) ONPG-Assay</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#XGAL">2) X-Gal-Assay</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#GFP">3) GFP-Reporter Test</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#Outdoor">Outdoor Test</a></li><br />
<li><a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement#Wrapup">Wrap-up</a></li><br />
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</html></div>Dniopekhttp://2012hs.igem.org/File:RecA_GFP_microscopy_HD2012.pngFile:RecA GFP microscopy HD2012.png2012-07-18T10:39:34Z<p>Dniopek: uploaded a new version of &quot;File:RecA GFP microscopy HD2012.png&quot;</p>
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<div></div>Dniopekhttp://2012hs.igem.org/File:RecA_GFP_microscopy_HD2012.pngFile:RecA GFP microscopy HD2012.png2012-07-18T10:38:41Z<p>Dniopek: </p>
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<div></div>Dniopekhttp://2012hs.igem.org/File:Setup_RecA_GFP_Microscopy_HD.pngFile:Setup RecA GFP Microscopy HD.png2012-07-18T10:35:47Z<p>Dniopek: uploaded a new version of &quot;File:Setup RecA GFP Microscopy HD.png&quot;</p>
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<div></div>Dniopekhttp://2012hs.igem.org/File:Setup_RecA_GFP_Microscopy_HD.pngFile:Setup RecA GFP Microscopy HD.png2012-07-18T10:33:19Z<p>Dniopek: uploaded a new version of &quot;File:Setup RecA GFP Microscopy HD.png&quot;</p>
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<div></div>Dniopekhttp://2012hs.igem.org/File:GFP_Measurment_HD2012.jpgFile:GFP Measurment HD2012.jpg2012-07-18T10:32:38Z<p>Dniopek: </p>
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<div></div>Dniopekhttp://2012hs.igem.org/File:Setup_RecA_GFP_Microscopy_HD.pngFile:Setup RecA GFP Microscopy HD.png2012-07-18T10:31:20Z<p>Dniopek: </p>
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<div></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_UVsensorsTeam:Heidelberg LSL/Project UVsensors2012-07-18T10:27:36Z<p>Dniopek: </p>
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<h2>Sensor Construction</h2><br />
<br />
<h4>Overview</h4><br />
<br />
<div class="img"><br />
<img src="https://static.igem.org/mediawiki/2012hs/b/b4/IGEMS_sensory-construction_overview.png" alt="Sensory Construction Overview" width="650"/></div><br />
<br> <br />
<p> SOS response promoters from <i>E.coli</i> were either annealed from synthetic oligos or taken from the <a href="http://partsregistry.org/Main_Page">partsregistry</a>. Promoters were ligated together with digested standard reporter constructs thereby generating our final radiation sensor constructs.</p><br />
<br />
<br />
<h4>Description</h4><br />
<p>It was intended to generate constructs that are able to sense irradiation and respond to UV light with a colorful output. For details of the cloning procedure, please refer to our <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Notebook">notebook</a>.</p><br />
<br />
<p>First, we looked for proper <i>E. coli</i> promoters known to be involved in the SOS response. While recB, recC and sulA promoters were generated with custom-made oligonucleotides, we took precA as biobrick <a href="http://partsregistry.org/Part:BBa_J22106">BBa_J22106</a>. We designed the oligos for precB, precC and psulA to contain <a href="http://dspace.mit.edu/bitstream/handle/1721.1/45138/BBFRFC10.txt?sequence=1"> BBa</a> standard prefix and suffix and putative promoter sequences (approx. 50 bp upstream of the start codon of each gene) taken from the <a href="http://ecoliwiki.net/Main_Page">EcoliWiki</a>. Oligos were annealed and then directly used for ligation since they already contained the required overhangs. The precA part was therefore digested with EcoRI and SpeI.<br />
<br><br />
As reporters, we decided for GFP and LacZ from the biobricks <a href="http://partsregistry.org/Part:BBa_E0240">BBa_E0240</a> and <a href="http://partsregistry.org/Part:BBa_K173004">BBa_K173004</a>, respectively. We digested those plasmids with EcoRI and XbaI to allow for ligation with promoters via standard assembly.<br />
<br><br />
Finally, constructs were transformed into <i>E.coli</i> strain BL21 and applied to sense UV radiation with visible output.<br />
Prior to submission, parts were transferred into <a href="http://partsregistry.org/Part:pSB1C3">pSB1C3 backbone</a>.</p><br />
<br />
<h4>Details</h4><br />
<p>The following constructs were created by the above explained strategy.</p><br />
<br />
<center><br />
<table class="wikitable sortable" border="0" style="text-align:center width="650px"><br />
<caption align="top, center"><br />
</caption><tr bgcolor="#cccccc"><br />
<td><b>Number</b><br></td><br />
<td><b>Promoter</b><br><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/></td><br />
<td><b>Reporter</b><br><img src="http://partsregistry.org/images/partbypart/icon_coding.png"/></td><br />
<td><b>Backbone</b><br><img src="http://partsregistry.org/images/partbypart/icon_plasmid_backbone.png"/></td><br />
</tr><br />
<tr><br />
<td>01</td><br />
<td><a href="http://partsregistry.org/Part:BBa_J22106">precA</a></td><br />
<td><a href="http://partsregistry.org/Part:BBa_E0040">gfp</a></td><br />
<td><a href="http://partsregistry.org/Part:pSB1K3">pSB1K3</a></td><br />
</tr><br />
<tr><br />
<td>02</td><br />
<td><a href="http://partsregistry.org/Part:BBa_K518010">psulA</a></td><br />
<td><a href="http://partsregistry.org/Part:BBa_E0040">gfp</a></td><br />
<td><a href="http://partsregistry.org/Part:pSB1K3">pSB1K3</a></td><br />
</tr><br />
<tr><br />
<td>03</td><br />
<td><a href="http://partsregistry.org/Part:BBa_J22106">precA</a></td><br />
<td><a href="http://partsregistry.org/Part:BBa_I732005">lacZ</a></td><br />
<td><a href="http://partsregistry.org/Part:pSB1K3">pSB1K3</a></td><br />
</tr><br />
<tr><br />
<td>04</td><br />
<td><a href="http://partsregistry.org/Part:BBa_K862003">precB</a></td><br />
<td><a href="http://partsregistry.org/Part:BBa_I732005">lacZ</a></td><br />
<td><a href="http://partsregistry.org/Part:pSB1K3">pSB1K3</a></td><br />
</tr><br />
<tr><br />
<td>05</td><br />
<td>precC</td><br />
<td><a href="http://partsregistry.org/Part:BBa_I732005">lacZ</a></td><br />
<td><a href="http://partsregistry.org/Part:pSB1K3">pSB1K3</a></td><br />
</tr><br />
<tr><br />
<td>06</td><br />
<td><a href="http://partsregistry.org/Part:BBa_K518010">psulA</a></td><br />
<td><a href="http://partsregistry.org/Part:BBa_I732005">lacZ</a></td><br />
<td><a href="http://partsregistry.org/Part:pSB1K3">pSB1K3</a></td><br />
</tr><br />
<tr><br />
<td>07</td><br />
<td><a href="http://partsregistry.org/Part:BBa_J22106">precA</a></td><br />
<td><a href="http://partsregistry.org/Part:BBa_I732005">lacZ</a></td><br />
<td><a href="http://partsregistry.org/Part:pSB1C3">pSB1C3</a></td><br />
</tr><br />
<td>08</td><br />
<td><a href="http://partsregistry.org/Part:BBa_K862003">precB</a></td><br />
<td><a href="http://partsregistry.org/Part:BBa_I732005">lacZ</a></td><br />
<td><a href="http://partsregistry.org/Part:pSB1C3">pSB1C3</a></td><br />
</tr><br />
<td>09</td><br />
<td><a href="http://partsregistry.org/Part:BBa_K518010">psulA</a></td><br />
<td><a href="http://partsregistry.org/Part:BBa_I732005">lacZ</a></td><br />
<td><a href="http://partsregistry.org/Part:pSB1C3">pSB1C3</a></td><br />
</tr><br />
</table><br />
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<h4>Sensor constructs</h4><br />
<p>Those constructs were successfully generated, characterized and submitted as <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Parts">parts</a>.</p><br />
<center><br />
<br />
<div class="img"><br />
<a href="https://static.igem.org/mediawiki/2012hs/b/b7/RecA_LacZ.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012hs/b/b7/RecA_LacZ.jpg" width="250"/></a><br />
<div class="desc1"> Map based on <a href="https://static.igem.org/mediawiki/2012hs/0/07/RecA_LacZ_pSB1C3.gb">precA_lacZ_pSB1C3</a>.</div><br />
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<a href="https://static.igem.org/mediawiki/2012hs/a/a1/RecB_LacZ.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012hs/a/a1/RecB_LacZ.jpg" width="250"/></a><br />
<div class="desc1"> Map based on <a href="https://static.igem.org/mediawiki/2012hs/1/1d/RecB_LacZ_pSB1C3.gb">precB_lacZ_pSB1C3</a>.</div><br />
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<a href="https://static.igem.org/mediawiki/2012hs/6/64/SulA_LacZ.jpg"><br />
<img src="https://static.igem.org/mediawiki/2012hs/6/64/SulA_LacZ.jpg" width="250"/></a><br />
<div class="desc1"> Map based on <a href="https://static.igem.org/mediawiki/2012hs/d/d3/SulA_LacZ_pSB1C3.gb">psulA_lacZ_pSB1C3</a>.</div><br />
</center><br />
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/p><br />
a href="https://static.igem.org/mediawiki/2012hs/0/07/RecA_LacZ_pSB1C3.gb">precA_lacZ_pSB1C3</div>Dniopekhttp://2012hs.igem.org/File:Promega_logo_180.jpgFile:Promega logo 180.jpg2012-07-18T10:24:32Z<p>Dniopek: uploaded a new version of &quot;File:Promega logo 180.jpg&quot;</p>
<hr />
<div></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/SponsorsTeam:Heidelberg LSL/Sponsors2012-07-18T10:22:57Z<p>Dniopek: </p>
<hr />
<div>{{TopII}}<br />
{{Stylesheet}}<br />
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<html><br />
<br />
<style type="text/css"> <br />
<br />
#news {min-height: 1750px;}<br />
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#bodyContent { background-color: transparent; border: none; width: 975px; min-height: 1750px;}<br />
.gallery img {margin: 0 15px 15px 17px;<br />
border-color: #FFF; <br />
border-style:solid; <br />
text-align:center;<br />
float:left;}<br />
.sponsors_img {<br />
width: 150px;<br />
background-color: transparent;<br />
border: none; <br />
}<br />
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</style><br />
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<body id="sponsors" onload="setPageSize()"><br />
<div id="super_main_wrapper"><br />
<div id="SubWrapper"><br />
<br />
<h2>Team-Sponsors</h2><br />
<!--<h4>Platinum Level</h4><br />
<br />
<br />
--><br />
<div class="gallery" style="margin-top: 1em;"><br />
<br />
<central><br />
<img src="https://static.igem.org/mediawiki/2012hs/e/e7/Gruppe6.png" width="640" style="margin-left:15px;"></img><br />
</central><br />
<br />
<br />
<a href="http://www.abbott.de/content/index_de.html"><h3>Abbott</h3></a><br />
<p>Abbott is a global, broad-based health care company devoted to discovering new medicines, new technologies and new ways to manage health. Their products span the continuum of care, from nutritional products and laboratory diagnostics through medical devices and pharmaceutical therapies. furthermore their comprehensive line of products encircles life itself - addressing important health needs from infancy to the golden years.<br />
<br />
Abbott has sales, manufacturing, research and development, and distribution facilities around the world, close to where our customers need us to be. They are recognized for our global reach and our ability to serve our customers around the world.<p/><br />
</div><br />
<br />
<div class="gallery" style="margin-top: 6em;"><br />
<a href="http://www.promega.com/"><h3>Promega</h3></a><br />
<p><br />
Promega Corporation is a leader in providing innovative solutions and technical support to the life-sciences industry. The company’s 2,000 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA with branches in 15 countries and over 50 global distributors. For more information about Promega, visit <a href="http://www.promega.com/"> www.promega.com</a>.<br />
</p><br />
</div><br />
<br />
<div class="gallery" style="margin-top: 1.9em;"><br />
<h4>Academic Sponsors</h4><br />
</div><br />
<br />
<div class="gallery" style="margin-top: 0em;"><br />
<a href="http://www.dkfz.de/en/index.html"><h3>DKFZ</h3></a><br />
<p><br />
The DKFZ (German Cancer Research Center) kindly refunded traveling costs of two of the advisors who are currently pursuing their PhD in the Helmholtz International Graduate School for Cancer Research.<br />
</p><br />
</a><br />
</div><br />
<br />
<br />
<div class="gallery" style="margin-top: 10em;"><br />
<h3>Jugendstiftung Baden-Württemberg</h3><br />
</div><br />
<!--<p><br />
Wir setzen Ideen in Konzepte und Projekte um. Wir mischen uns ein, fördern und gestalten.<br />
Wir geben Anstöße, suchen und unterstützen Jugendliche mit Ideen, Engagement und Idealismus.</p>--><br />
<p>The "Jugendstiftung Baden-Württemberg" implements ideas into concepts and projects. It intervenes, sponsors and arranges. <br /><br />
The "Jugendstiftung Baden-Württemberg" looks for young people with ideas, commitment and idealism and gives impulses to support them.</p><br />
<br />
<div class="gallery" style="margin-top: 10em;"><br />
<h3>Alumni des Heidelberger Life-Science Lab e.V.</h3><br />
</div><br />
<!--<p>Seit seiner Gründung im Jahre 2005 vernetzt der Verein der "Alumni des Heidelberger Life-Science Lab e.V" Mentoren, Teilnehmer und Ehemalige des 1999 gegründeten Life-Science Lab. Wir haben es uns zur Aufgabe gemacht neben der Förderung des Life-Science Lab unseren rund 300 Mitgliedern wissenschaftliche Akademien und Wochenendseminare zu verschiedenen Themen anzubieten und den Austausch zwischen Ehemaligen und Lab-Teilnehmern zu verstärken.</p>--><br />
<p>Since its foundation in 2005 the Association of the "Alumni des Heidelberger Life-Science Lab e.V." connects participating students, mentors and former participants of the Heidelberg Life-Science Lab. <br /> It is our task not only to promote and support the students during their activities but also to offer scientific Academies and Seminars to the 300 participants as well as to reinforce the exchange between Lab-participants and Alumni.</p><br />
<br />
<div class="gallery" style="margin-top: 9em;"><br />
<h3>Freundeskreis Englisches Institut Heidelberg e.V.</h3><br />
</div><br />
<p>The "Freundeskreis des Englischen Instituts" was established in 1973 and is a community of parents, students, teachers and alumni supporting financially and materially educational life at the private high school Englisch Institute in Heidelberg. Mariam Harmouche is a member of the English Institute and is grateful to be sponsored by the Freundeskreis e.V which enables her to take part at the iGEM Jamboree in June 2012.</p><br />
<br />
<div class="gallery" style="margin-top: 7em;"><br />
<h3>Charlottes´ Granny</h3><br />
</div><br />
<p>One of the members, Charlotte Bunne, is sponsored by her grandma, too. At her last visit she told her grandma about her participation at the iGEM Jamboree. Her grandma was fascinated and supports her with a small amount. </p><br />
<p>&nbsp;</p><br />
<br />
<br />
</div><!--end SubWrapper--><br />
<br />
<br />
<div id="news"> <br />
<center><br />
<!--<div class="sponsors_img" style="margin-top: 22em;"><br />
<a href="http://www.mckinsey.de/html/home/index.asp"><img src="https://static.igem.org/mediawiki/2012hs/a/ae/McKinsey.png"/></a><br />
</div><br />
--><br />
<br />
<br />
<div class="sponsors_img" style="margin-top: 22em;"><br />
<a href="http://www.abbott.de/content/index_de.html"><img src="https://static.igem.org/mediawiki/2012hs/3/30/Abbott.jpg"/></a><br />
</div><br />
<br />
<br />
<div class="sponsors_img" style="margin-top: 7em;"><br />
<a href="http://www.promega.com/"><img src="https://static.igem.org/mediawiki/2012hs/d/d5/Promega_logo_180.jpg" width="180" height="100"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 8em;"><br />
<a href="http://www.dkfz.de/en/index.html"><img src="https://static.igem.org/mediawiki/2012hs/a/a9/Dkfz.jpg"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 12em;"><br />
<a href="http://www.jugendstiftung.de/"><img src="https://static.igem.org/mediawiki/2012hs/f/f3/JugendstiftungBW-logo.gif"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 10em;"><br />
<a href="http://www.lab-alumni.org/"><img src="https://static.igem.org/mediawiki/2012hs/c/c9/Lab-alumni-logo.png"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 8em;"><br />
<a href="http://www.fei-hd.de/"><img src="https://static.igem.org/mediawiki/2012hs/3/3c/FEI-Logo-oT.jpg" width=120px"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 6em;"><br />
<a href="#"><img src="https://static.igem.org/mediawiki/2012hs/6/6c/Grandma.jpg" width=120px"/></a><br />
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<script type="text/javascript"><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/SponsorsTeam:Heidelberg LSL/Sponsors2012-07-18T10:20:50Z<p>Dniopek: </p>
<hr />
<div>{{TopII}}<br />
{{Stylesheet}}<br />
<br />
<html><br />
<br />
<style type="text/css"> <br />
<br />
#news {min-height: 1750px;}<br />
<br />
#bodyContent { background-color: transparent; border: none; width: 975px; min-height: 1750px;}<br />
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border-color: #FFF; <br />
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float:left;}<br />
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width: 150px;<br />
background-color: transparent;<br />
border: none; <br />
}<br />
<br />
</style><br />
<br />
<body id="sponsors" onload="setPageSize()"><br />
<div id="super_main_wrapper"><br />
<div id="SubWrapper"><br />
<br />
<h2>Team-Sponsors</h2><br />
<!--<h4>Platinum Level</h4><br />
<br />
<br />
--><br />
<div class="gallery" style="margin-top: 1em;"><br />
<br />
<central><br />
<img src="https://static.igem.org/mediawiki/2012hs/e/e7/Gruppe6.png" width="640" style="margin-left:15px;"></img><br />
</central><br />
<br />
<br />
<a href="http://www.abbott.de/content/index_de.html"><h3>Abbott</h3></a><br />
<p>Abbott is a global, broad-based health care company devoted to discovering new medicines, new technologies and new ways to manage health. Their products span the continuum of care, from nutritional products and laboratory diagnostics through medical devices and pharmaceutical therapies. furthermore their comprehensive line of products encircles life itself - addressing important health needs from infancy to the golden years.<br />
<br />
Abbott has sales, manufacturing, research and development, and distribution facilities around the world, close to where our customers need us to be. They are recognized for our global reach and our ability to serve our customers around the world.<p/><br />
</div><br />
<br />
<div class="gallery" style="margin-top: 6em;"><br />
<a href="http://www.promega.com/"><h3>Promega</h3></a><br />
<p><br />
Promega Corporation is a leader in providing innovative solutions and technical support to the life-sciences industry. The company’s 2,000 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA with branches in 15 countries and over 50 global distributors. For more information about Promega, visit <a href="http://www.promega.com/"> www.promega.com</a>.<br />
</p><br />
</div><br />
<br />
<div class="gallery" style="margin-top: 1.9em;"><br />
<h4>Academic Sponsors</h4><br />
</div><br />
<br />
<div class="gallery" style="margin-top: 0em;"><br />
<a href="http://www.dkfz.de/en/index.html"><h3>DKFZ</h3></a><br />
<p><br />
The DKFZ (German Cancer Research Center) kindly refunded traveling costs of two of the advisors who are currently pursuing their PhD in the Helmholtz International Graduate School for Cancer Research.<br />
</p><br />
</a><br />
</div><br />
<br />
<br />
<div class="gallery" style="margin-top: 10em;"><br />
<h3>Jugendstiftung Baden-Württemberg</h3><br />
</div><br />
<!--<p><br />
Wir setzen Ideen in Konzepte und Projekte um. Wir mischen uns ein, fördern und gestalten.<br />
Wir geben Anstöße, suchen und unterstützen Jugendliche mit Ideen, Engagement und Idealismus.</p>--><br />
<p>The "Jugendstiftung Baden-Württemberg" implements ideas into concepts and projects. It intervenes, sponsors and arranges. <br /><br />
The "Jugendstiftung Baden-Württemberg" looks for young people with ideas, commitment and idealism and gives impulses to support them.</p><br />
<br />
<div class="gallery" style="margin-top: 10em;"><br />
<h3>Alumni des Heidelberger Life-Science Lab e.V.</h3><br />
</div><br />
<!--<p>Seit seiner Gründung im Jahre 2005 vernetzt der Verein der "Alumni des Heidelberger Life-Science Lab e.V" Mentoren, Teilnehmer und Ehemalige des 1999 gegründeten Life-Science Lab. Wir haben es uns zur Aufgabe gemacht neben der Förderung des Life-Science Lab unseren rund 300 Mitgliedern wissenschaftliche Akademien und Wochenendseminare zu verschiedenen Themen anzubieten und den Austausch zwischen Ehemaligen und Lab-Teilnehmern zu verstärken.</p>--><br />
<p>Since its foundation in 2005 the Association of the "Alumni des Heidelberger Life-Science Lab e.V." connects participating students, mentors and former participants of the Heidelberg Life-Science Lab. <br /> It is our task not only to promote and support the students during their activities but also to offer scientific Academies and Seminars to the 300 participants as well as to reinforce the exchange between Lab-participants and Alumni.</p><br />
<br />
<div class="gallery" style="margin-top: 9em;"><br />
<h3>Freundeskreis Englisches Institut Heidelberg e.V.</h3><br />
</div><br />
<p>The "Freundeskreis des Englischen Instituts" was established in 1973 and is a community of parents, students, teachers and alumni supporting financially and materially educational life at the private high school Englisch Institute in Heidelberg. Mariam Harmouche is a member of the English Institute and is grateful to be sponsored by the Freundeskreis e.V which enables her to take part at the iGEM Jamboree in June 2012.</p><br />
<br />
<div class="gallery" style="margin-top: 7em;"><br />
<h3>Charlottes´ Granny</h3><br />
</div><br />
<p>One of the members, Charlotte Bunne, is sponsored by her grandma, too. At her last visit she told her grandma about her participation at the iGEM Jamboree. Her grandma was fascinated and supports her with a small amount. </p><br />
<p>&nbsp;</p><br />
<br />
<br />
</div><!--end SubWrapper--><br />
<br />
<br />
<div id="news"> <br />
<center><br />
<!--<div class="sponsors_img" style="margin-top: 22em;"><br />
<a href="http://www.mckinsey.de/html/home/index.asp"><img src="https://static.igem.org/mediawiki/2012hs/a/ae/McKinsey.png"/></a><br />
</div><br />
--><br />
<br />
<br />
<div class="sponsors_img" style="margin-top: 22em;"><br />
<a href="http://www.abbott.de/content/index_de.html"><img src="https://static.igem.org/mediawiki/2012hs/3/30/Abbott.jpg"/></a><br />
</div><br />
<br />
<br />
<div class="sponsors_img" style="margin-top: 7em;"><br />
<a href="http://www.promega.com/"><img src="http://2012HS.igem.org/wiki/images/a/ab/Promega_logo_180.jpg" width="180" height="100"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 8em;"><br />
<a href="http://www.dkfz.de/en/index.html"><img src="https://static.igem.org/mediawiki/2012hs/a/a9/Dkfz.jpg"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 12em;"><br />
<a href="http://www.jugendstiftung.de/"><img src="https://static.igem.org/mediawiki/2012hs/f/f3/JugendstiftungBW-logo.gif"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 10em;"><br />
<a href="http://www.lab-alumni.org/"><img src="https://static.igem.org/mediawiki/2012hs/c/c9/Lab-alumni-logo.png"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 8em;"><br />
<a href="http://www.fei-hd.de/"><img src="https://static.igem.org/mediawiki/2012hs/3/3c/FEI-Logo-oT.jpg" width=120px"/></a><br />
</div><br />
<br />
<div class="sponsors_img" style="margin-top: 6em;"><br />
<a href="#"><img src="https://static.igem.org/mediawiki/2012hs/6/6c/Grandma.jpg" width=120px"/></a><br />
</div><br />
</center><br />
<br />
</div> <!--news--><br />
</div> <!-- end super_main_wrapper><br />
</body><br />
<br />
<script type="text/javascript"><br />
function setPageSize() {<br />
len = document.getElementById('super_main_wrapper').offsetHeight;<br />
document.getElementById('bodyContent').style.height = len + 'px';<br />
document.getElementById('news').style.height = len + 'px';<br />
}<br />
</script><br />
</html></div>Dniopekhttp://2012hs.igem.org/File:Promega_logo_180.jpgFile:Promega logo 180.jpg2012-07-18T10:19:12Z<p>Dniopek: </p>
<hr />
<div></div>Dniopekhttp://2012hs.igem.org/IGEM_PublicityIGEM Publicity2012-07-12T18:52:07Z<p>Dniopek: </p>
<hr />
<div>'''Team Heidelberg LSL'''<br />
<br />
* [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=152850.html Biotechnologie.de news]<br />
* [http://www.bio-pro.de/schule/artikelliste/index.html?lang=de&artikelid=/artikel/08222/index.html Biopro news]<br />
* [http://www.laborwelt.de/aktuelles/nachrichten/2012-q3/igem-sieg-mit-mikrobenschmuck.html Laborwelt.de]<br />
* [http://www.biotechnologie.de/BIO/Navigation/DE/Aktuelles/biotechnologie-tv,did=152228.html?listBlId=85084& Biotechnologie.tv video interview (starting min 3:55)]<br />
* [http://www.mensch-und-krebs.de/index.php/krebsthemen/hautkrebs/867-schmucke-bakterien-gegen-hautkrebs.html Mensch & Krebs]<br />
* [http://www.ihre-vorsorge.de/magazin/nachrichten/rente/news-single/article/bakterienschmuck-gegen-hautkrebs.html Ihre-Vorsorge online magasine]<br />
* [http://www.dw.de/dw/article/0,,16088170,00.html?maca=de-rss-de-all-1119-rdf article Deutsche Welle]</div>Dniopekhttp://2012hs.igem.org/IGEM_PublicityIGEM Publicity2012-07-12T15:13:58Z<p>Dniopek: </p>
<hr />
<div>'''Team Heidelberg LSL'''<br />
<br />
* [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=152850.html Biotechnologie.de news]<br />
* [http://www.bio-pro.de/schule/artikelliste/index.html?lang=de&artikelid=/artikel/08222/index.html Biopro news]<br />
* [http://www.laborwelt.de/aktuelles/nachrichten/2012-q3/igem-sieg-mit-mikrobenschmuck.html Laborwelt.de]<br />
* [http://www.biotechnologie.de/BIO/Navigation/DE/Aktuelles/biotechnologie-tv,did=152228.html?listBlId=85084& Biotechnologie.tv video interview (starting min 3:55)]<br />
* [http://www.mensch-und-krebs.de/index.php/krebsthemen/hautkrebs/867-schmucke-bakterien-gegen-hautkrebs.html Mensch & Krebs]<br />
* [http://www.ihre-vorsorge.de/magazin/nachrichten/rente/news-single/article/bakterienschmuck-gegen-hautkrebs.html Ihre-Vorsorge online magasine]<br />
* [http://www.dw.de/dw/article/0,,16088170,00.html?maca=de-rss-de-all-1119-rdf Bericht Deutsche Welle]</div>Dniopekhttp://2012hs.igem.org/IGEM_PublicityIGEM Publicity2012-07-11T20:32:35Z<p>Dniopek: </p>
<hr />
<div>'''Team Heidelberg LSL'''<br />
<br />
* [http://www.biotechnologie.de/BIO/Navigation/DE/root,did=152850.html Biotechnologie.de news]<br />
* [http://www.bio-pro.de/schule/artikelliste/index.html?lang=de&artikelid=/artikel/08222/index.html Biopro news]<br />
* [http://www.laborwelt.de/aktuelles/nachrichten/2012-q3/igem-sieg-mit-mikrobenschmuck.html Laborwelt.de]<br />
* [http://www.biotechnologie.de/BIO/Navigation/DE/Aktuelles/biotechnologie-tv,did=152228.html?listBlId=85084& Biotechnologie.tv video interview (starting min 3:55)]<br />
* [http://www.mensch-und-krebs.de/index.php/krebsthemen/hautkrebs/867-schmucke-bakterien-gegen-hautkrebs.html Mensch & Krebs]<br />
* [http://www.ihre-vorsorge.de/magazin/nachrichten/rente/news-single/article/bakterienschmuck-gegen-hautkrebs.html Ihre-Vorsorge online magasine]</div>Dniopekhttp://2012hs.igem.org/Jamboree/Schedule/Practice_SessionsJamboree/Schedule/Practice Sessions2012-06-26T17:00:19Z<p>Dniopek: </p>
<hr />
<div><html><br />
<style type="text/css"><br />
h2 {<br />
border-bottom-width: 0px;<br />
border-bottom-style: none;<br />
margin-bottom: 0px;<br />
}<br />
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display: none;<br />
}<br />
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table.calendar {<br />
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font-size: 12px;<br />
border-bottom: 2px solid #AAAAAA;<br />
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<br />
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text-align: center;<br />
border-bottom: 2px solid #AAAAAA;<br />
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font-weight: bold;<br />
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table.calendar tbody {<br />
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table.calendar tbody th {<br />
color: #494949;<br />
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border-bottom: 1px solid #DDDDDD;<br />
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table.calendar tr:hover { <br />
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}<br />
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table.calendar td {<br />
text-align: center;<br />
border: 1px solid #DDDDDD;<br />
}<br />
</style><br />
</html><br />
If you'd like to practice your presentations on Friday (June 29th), we'll have rooms available for use. You can edit this page (hover at the top left corner, above the iGEM logo), and reserve a spot for your team in the table below. <br />
<br />
<br />
There are a limited number of time slots available on a first-come first-serve basis so choose only one slot. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time! If you're having trouble reserving a spot, send an email to <b>hq (at) igem (dot) org</b> with the time and room you'd like to be scheduled for. <br />
<br />
<br />
'''Note:''' We cannot match the room that you will ultimately give your presentation in with the practice room. There will NOT be any A/V (audio/visual) support on staff. All classrooms will be unlocked and you should use them and leave them as you found them. <br />
<br />
<br />
There will be pizza and refreshments as well! So come early before it runs out!<br />
<br><br><br />
<br />
<br />
<html> <!--Practice Session Sign-Up Sheet --><br />
<table class="calendar" align="center"><h2 class="date"><a name="Friday Practice">Friday, November 4</a></h2><br />
<thead><br />
<tr><br />
<th style="width:100px;">Time</th><br />
<th>CMR </th><br />
<th>Blue and Gold Room </th><br />
<th>Media Center</th><br />
</tr><br />
</thead><br />
<tbody><br />
<tr class="even"><br />
<th>6:00p - 6:30p</th><br />
<td>C-1</td><br />
<td>B-1</td><br />
<td>M-1</td><br />
</tr><br />
<tr class="odd"><br />
<th>6:30p - 7:00p</th><br />
<td>C-2</td><br />
<td>B-2</td><br />
<td>M-2</td><br />
</tr><br />
<tr class="even"><br />
<th>7:00p - 7:30p</th><br />
<td>C-3</td><br />
<td>B-3</td><br />
<td>M-3</td><br />
</tr><br />
<tr class="even"><br />
<th>7:30p - 8:00p</th><br />
<td>C-4</td><br />
<td>Heidelberg_LSL</td><br />
<td>M-4</td><br />
</tr><br />
</tbody><br />
</table><br />
</html></div>Dniopekhttp://2012hs.igem.org/Jamboree/Schedule/Practice_SessionsJamboree/Schedule/Practice Sessions2012-06-26T17:00:04Z<p>Dniopek: </p>
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If you'd like to practice your presentations on Friday (June 29th), we'll have rooms available for use. You can edit this page (hover at the top left corner, above the iGEM logo), and reserve a spot for your team in the table below. <br />
<br />
<br />
There are a limited number of time slots available on a first-come first-serve basis so choose only one slot. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time! If you're having trouble reserving a spot, send an email to <b>hq (at) igem (dot) org</b> with the time and room you'd like to be scheduled for. <br />
<br />
<br />
'''Note:''' We cannot match the room that you will ultimately give your presentation in with the practice room. There will NOT be any A/V (audio/visual) support on staff. All classrooms will be unlocked and you should use them and leave them as you found them. <br />
<br />
<br />
There will be pizza and refreshments as well! So come early before it runs out!<br />
<br><br><br />
<br />
<br />
<html> <!--Practice Session Sign-Up Sheet --><br />
<table class="calendar" align="center"><h2 class="date"><a name="Friday Practice">Friday, November 4</a></h2><br />
<thead><br />
<tr><br />
<th style="width:100px;">Time</th><br />
<th>CMR </th><br />
<th>Blue and Gold Room </th><br />
<th>Media Center</th><br />
</tr><br />
</thead><br />
<tbody><br />
<tr class="even"><br />
<th>6:00p - 6:30p</th><br />
<td>C-1</td><br />
<td>B-1</td><br />
<td>M-1</td><br />
</tr><br />
<tr class="odd"><br />
<th>6:30p - 7:00p</th><br />
<td>C-2</td><br />
<td>B-2</td><br />
<td>M-2</td><br />
</tr><br />
<tr class="even"><br />
<th>7:00p - 7:30p</th><br />
<td>C-3</td><br />
<td>B-3</td><br />
<td>M-3</td><br />
</tr><br />
<tr class="even"><br />
<th>7:30p - 8:00p</th><br />
<td>C-4</td><br />
<td>B-4 - Heidelberg_LSL</td><br />
<td>M-4</td><br />
</tr><br />
</tbody><br />
</table><br />
</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_humanPracticeTeam:Heidelberg LSL/Project humanPractice2012-06-17T03:12:41Z<p>Dniopek: </p>
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<br />
<h2>Human Practice</h2><br />
<br />
<br />
<p>As the Heidelberg Life-Science Lab is a place where science meets interested pupils, for us, it is very important to draw the connection between our scientific work and the non-scientific community. Many people are not aware of synthetic biology and only a minority is really informed about its potential. With our work, we want to encourage non-scientific readers to negotiate possible prejudices, learn more about our work in the lab and have an entertaining insight into the various applications of our new biological system.</p><br />
<br />
<br />
<h4>How can high school students learn about genetic engineering?</h4><br />
<br />
<br />
<p>Learning by heart belongs to the past. We are learning by doing. Being normal high-school students, we want to show that everybody can understand the basic principles of how genetic circuits in cells can be 'engineered' and how intelligible most of the methods are. With our many explanations and protocols, we want to support, encourage and introduce upcoming high-school teams, interested teenagers, students and adults without vast knowledge to get involved into synthetic and molecular biology.</p><br />
<br />
<br />
<h4>Science interesting and intelligible - why not writing a book?</h4><br />
<br />
<p>This is the idea and motivation of the popular German scientific writer Dr. Olaf Fritsche. His upcoming book deals with the future of biological sciences. He will explain how scientific knowledge arises and show that everybody can understand facts when asking the right questions in science. Olaf approached us some weeks ago and asked whether he could visit our iGEM HS team in order to ask some basic questions about our project and team. Finally, he did not only visit us once, but several times, both inside and outside of the laboratory and followed our project from its start with the idea to its final end (his last visit was in the wiki-freeze night and he will follow up also after the iGEM jamboree). Its our great pleasure and honor, that Olaf Fritsche will report about our teams' and projects' development during taking part in the iGEM HS competition in his book in several passages embedded in different chapters. Fritsche’s work will show that establishing an iGEM project extra-curricularly is ambitious but in the same way funny and intelligible. We hope that his book will encourage people outside of the synthetic biology community to overcome prejudices against science, in particular molecular biology and synthetic biology and become more educated and informed about modern life-sciences developments.</p><br />
<br />
<br />
<h4>Why iGEMs and an Online Shop?</h4><br />
<br />
<p>We carefully thought about possible applications of our UV-sensing bacteria which would - for sure - be in the health-protection sector, e.g. by warning people when getting sun-burned by a high UV dose. We decided to work in direction of an everyday-life product of which the general public should have most profit from.<br />
For the integration of our measurement construct containing bacteria into such a product context, we thought about a funny and innovative way that could be coherent with current trends and that should evoke interest and curiosity amongst people. iGEMs, genetically modified gems, on bracelets and necklaces are a nice way to communicate our ideas to a broad public.<br/><br />
<br />
The way we communicate our products is, for that reason, an online store <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Online_store">‘Online store’</a> which is - of course - not a real store, but just a fake (you can neither buy the iGEMS, nor the UV-sensing bacteria or anything). The store is supposed to give the user a reality-like impression of an everyday-life product which could be developed by using synthetic biology approaches. To overcome the distance to our product, we tried to imitate the common structure and design that the user knows from other online-shops and stores. However, when the user gets interested into a certain product and wants to buy it, we clarify, that our shop is just a 'fake' and aims for raising interest in synthetic biology and inform about this new scientific area. We provide the user with website links that provide information about iGEM and synthetic biology. In addition, we implemented a simple contact form, by which the user can get in touch with us in order to get more information about our project. <br><br />
</p><br />
<br />
<h4>Watch us on biotechnologie.tv! (in German language)</h4><br />
<br />
<p>We are very happy that the online information platform ‘biotechnologie.de’ recorded an interview with one of our participants, Charlotte Bunne, and included it into one of their weekly documentations recently. Initiated by the German ministry for education and science, <br />
<a href="http://www.biotechnologie.de/">biotechnologie.de</a><br />
is a public platform to inform not only scientists, but also people without scientific background about current results and technologies in biotechnological research. One of its main sections is a large video collection with short videos about various areas associated with biotechnology. The platform is constantly updated and revised and we are very glad to be in the latest online episode of biotechnologie.tv. Here, Charlotte explains briefly and very intelligible our iGEM HS project idea, the concept behind and what we are doing right now. You can watch the interview at the bottom of the page. It starts at 3:55 min.</p></br><br />
<br />
<iframe width="600px" height="400px" style="margin-left:20px;" src="http://www.youtube.com/embed/FxsJ8zEONTs" frameborder="0" allowfullscreen ><p>If the video doesn't appear here, please try reloading this page once or use the link below!</p></iframe></br></br><br />
<p> If the video doesn't appear here, please try reloading this page once or use the following link!</p><br />
<p>Link to the video: <a href="http://www.youtube.com/embed/FxsJ8zEONTs">http://www.youtube.com/embed/FxsJ8zEONTs</a></p><br />
<br />
</div><!--end news--><br />
<div id="news"><br />
<center><br />
<p><br />
<center><br />
<br />
<div class="img"><br />
<a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Team"><br />
<img src="https://static.igem.org/mediawiki/2012hs/6/63/IGEMS-Team1roundcorners.jpg" alt="Team" width="210"/><br />
<br />
</center><br />
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<img style="border:1px solid grey; border-radius: 10px;" src=" https://static.igem.org/mediawiki/2012hs/8/88/LifeScience_Lab_0027-klein.jpg " alt="Team" width="210"/><br />
<br />
</center><br />
</p><br />
<br />
<p><br />
<center><br />
<br />
<div class="img"><br />
<a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Gallery"><br />
<img style="border:1px solid grey; border-radius:8px;" src="https://static.igem.org/mediawiki/2012hs/8/80/DSC04439.JPG" alt="Team" width="210"/><br />
<br />
</center><br />
</p><p><br />
<center><br />
<br />
<div class="img"><br />
<a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Online_store"><br />
<img style="border:1px solid grey;" src="https://static.igem.org/mediawiki/2012hs/d/dd/Online-store-link.png" alt="Team" width="210"/><br />
<br />
</center><br />
</p><br />
<p><br />
<center><br />
<br />
<div class="img"><br />
<a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Sundra_col"><br />
<img style="border:1px solid grey;" src="https://static.igem.org/mediawiki/2012hs/9/93/PinkS.png" alt="Team" width="210"/><br />
<br />
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<hr />
<div>{{TopII}}<br />
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<div id="SubWrapper"><br />
<br />
<h2>Human Practice</h2><br />
<br />
<br />
<p>As the Heidelberg Life-Science Lab is a place where science meets interested pupils, for us, it is very important to draw the connection between our scientific work and the non-scientific community. Many people are not aware of synthetic biology and only a minority is really informed about its potential. With our work, we want to encourage non-scientific readers to negotiate possible prejudices, learn more about our work in the lab and have an entertaining insight into the various applications of our new biological system.</p><br />
<br />
<br />
<h4>How can high school students learn about genetic engineering?</h4><br />
<br />
<br />
<p>Learning by heart belongs to the past. We are learning by doing. Being normal high-school students, we want to show that everybody can understand the basic principles of how genetic circuits in cells can be 'engineered' and how intelligible most of the methods are. With our many explanations and protocols, we want to support, encourage and introduce upcoming high-school teams, interested teenagers, students and adults without vast knowledge to get involved into synthetic and molecular biology.</p><br />
<br />
<br />
<h4>Science interesting and intelligible - why not writing a book?</h4><br />
<br />
<p>This is the idea and motivation of the popular German scientific writer Dr. Olaf Fritsche. His upcoming book deals with the future of biological sciences. He will explain how scientific knowledge arises and show that everybody can understand facts when asking the right questions in science. Olaf approached us some weeks ago and asked whether he could visit our iGEM HS team in order to ask some basic questions about our project and team. Finally, he did not only visit us once, but several times, both inside and outside of the laboratory and followed our project from its start with the idea to its final end (his last visit was in the wiki-freeze night and he will follow up also after the iGEM jamboree). Its our great pleasure and honor, that Olaf Fritsche will report about our teams' and projects' development during taking part in the iGEM HS competition in his book in several passages embedded in different chapters. Fritsche’s work will show that establishing an iGEM project extra-curricularly is ambitious but in the same way funny and intelligible. We hope that his book will encourage people outside of the synthetic biology community to overcome prejudices against science, in particular molecular biology and synthetic biology and become more educated and informed about modern life-sciences developments.</p><br />
<br />
<br />
<h4>Why iGEMs and an Online Shop?</h4><br />
<br />
<p>We carefully thought about possible applications of our UV-sensing bacteria which would - for sure - be in the health-protection sector, e.g. by warning people when getting sun-burned by a high UV dose. We decided to work in direction of an everyday-life product of which the general public should have most profit.<br />
For the integration of our measurement construct containing bacteria into such a product context, we thought about a funny and innovative way that could be coherent with current trends and that should evoke interest and curiosity amongst people. iGEMs, genetically modified gems, on bracelets and necklaces are a nice way to communicate our ideas to a great public.<br/><br />
<br />
The way we communicate our products is, for that reason, an online store <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Online_store">‘Online store’</a> which is - of course - not a real store, but just a fake (you can neither buy the iGEMS, nor the UV-sensing bacteria or anything). The store is supposed to give the user a reality-like impression of an everyday-life product which could be developed by using synthetic biology approaches. We tried to give it an authentic outfit in order to show that such a shop could probably become reality in the future. To overcome the distance to our product, we tried to imitate the common structure and design that the user knows from other online-shops and stores. However, when the user gets interested into a certain product and wants to buy it, we clarify, that our shop is just a 'fake' and aims for raising interest in synthetic biology and inform about this new scientific approach. We provide the user with website links that provide information about iGEM and synthetic biology. In addition, we implemented a simple contact form, by which the user can get in touch with us in order to get more information about our project. <br><br />
</p><br />
<br />
<h4>Watch us on biotechnologie.tv! (in German language)</h4><br />
<br />
<p>We are very happy that the online information platform ‘biotechnologie.de’ recorded an interview with one of our participants, Charlotte Bunne, and included it into one of their weekly documentations recently. Initiated by the German ministry for education and science, <br />
<a href="http://www.biotechnologie.de/">biotechnologie.de</a><br />
is a public platform to inform not only scientists, but also people without scientific background about current results and technologies in biotechnological research. One of its main sections is a large video collection with short videos about various areas associated with biotechnology. The platform is constantly updated and revised and we are very glad to be in the latest online episode of biotechnologie.tv. Here, Charlotte explains briefly and very intelligible our iGEM HS project idea, the concept behind and what we are doing right now. You can watch the interview at the bottom of the page. It starts at 3:55 min.</p></br><br />
<br />
<iframe width="600px" height="400px" style="margin-left:20px;" src="http://www.youtube.com/embed/FxsJ8zEONTs" frameborder="0" allowfullscreen ><p>If the video doesn't appear here, please try reloading this page once or use the link below!</p></iframe></br></br><br />
<p> If the video doesn't appear here, please try reloading this page once or use the following link!</p><br />
<p>Link to the video: <a href="http://www.youtube.com/embed/FxsJ8zEONTs">http://www.youtube.com/embed/FxsJ8zEONTs</a></p><br />
<br />
</div><!--end news--><br />
<div id="news"><br />
<center><br />
<p><br />
<center><br />
<br />
<div class="img"><br />
<a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Team"><br />
<img src="https://static.igem.org/mediawiki/2012hs/6/63/IGEMS-Team1roundcorners.jpg" alt="Team" width="210"/><br />
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</center><br />
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<center><br />
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</center><br />
</p><br />
<p><br />
<center><br />
<br />
<div class="img"><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_humanPracticeTeam:Heidelberg LSL/Project humanPractice2012-06-17T03:08:55Z<p>Dniopek: </p>
<hr />
<div>{{TopII}}<br />
{{Stylesheet}}<br />
<br />
<html><br />
<br />
<style type="text/css"> <br />
<br />
<br />
#bodyContent {background-color: transparent; border: none; width: 975px; min-height: 1710px;}<br />
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<body id="sponsors" onload="setPageSize()"><br />
<div id="super_main_wrapper"><br />
<div id="SubWrapper"><br />
<br />
<h2>Human Practice</h2><br />
<br />
<br />
<p>As the Heidelberg Life-Science Lab is a place where science meets interested pupils, for us, it is very important to draw the connection between our scientific work and the non-scientific community. Many people are not aware of synthetic biology and only a minority is really informed about its potential. With our work, we want to encourage non-scientific readers to negotiate possible prejudices, learn more about our work in the lab and have an entertaining insight into the various applications of our new biological system.</p><br />
<br />
<br />
<h4>How can high-school students learn about genetic engineering?</h4><br />
<br />
<br />
<p>Learning by heart belongs to the past. We are learning by doing. Being normal high-school students, we want to show that everybody can understand the basic principles of how genetic circuits in cells can be 'engineered' and how intelligible most of the methods are. With our many explanations and protocols, we want to support, encourage and introduce upcoming high-school teams, interested teenagers, students and adults without vast knowledge to get involved into synthetic and molecular biology.</p><br />
<br />
<br />
<h4>Science interesting and intelligible - why not writing a book?</h4><br />
<br />
<p>This is the idea and motivation of the popular German scientific writer Dr. Olaf Fritsche. His upcoming book deals with the future of biological sciences. He will explain how scientific knowledge arises and show that everybody can understand facts when asking the right questions in science. Olaf approached us some weeks ago and asked whether he could visit our iGEM HS team in order to ask some basic questions about our project and team. Finally, he did not only visit us once, but several times, both inside and outside of the laboratory and followed our project from its start with the idea to its final end (his last visit was in the wiki-freeze night and he will follow up also after the iGEM jamboree). Its our great pleasure and honor, that Olaf Fritsche will report about our teams' and projects' development during taking part in the iGEM HS competition in his book in several passages embedded in different chapters. Fritsche’s work will show that establishing an iGEM project extra-curricularly is ambitious but in the same way funny and intelligible. We hope that his book will encourage people outside of the synthetic biology community to overcome prejudices against science, in particular molecular biology and synthetic biology and become more educated and informed about modern life-sciences developments.</p><br />
<br />
<br />
<h4>Why iGEMs and an Online Shop?</h4><br />
<br />
<p>We carefully thought about possible applications of our UV-sensing bacteria which would - for sure - be in the health-protection sector, e.g. by warning people when getting sun-burned by a high UV dose. We decided to work in direction of an everyday-life product of which the general public should have most profit.<br />
For the integration of our measurement construct containing bacteria into such a product context, we thought about a funny and innovative way that could be coherent with current trends and that should evoke interest and curiosity amongst people. iGEMs, genetically modified gems, on bracelets and necklaces are a nice way to communicate our ideas to a great public.<br/><br />
<br />
The way we communicate our products is, for that reason, an online store <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Online_store">‘Online store’</a> which is - of course - not a real store, but just a fake (you can neither buy the iGEMS, nor the UV-sensing bacteria or anything). The store is supposed to give the user a reality-like impression of an everyday-life product which could be developed by using synthetic biology approaches. We tried to give it an authentic outfit in order to show that such a shop could probably become reality in the future. To overcome the distance to our product, we tried to imitate the common structure and design that the user knows from other online-shops and stores. However, when the user gets interested into a certain product and wants to buy it, we clarify, that our shop is just a 'fake' and aims for raising interest in synthetic biology and inform about this new scientific approach. We provide the user with website links that provide information about iGEM and synthetic biology. In addition, we implemented a simple contact form, by which the user can get in touch with us in order to get more information about our project. <br><br />
</p><br />
<br />
<h4>Watch us on biotechnologie.tv! (in German language)</h4><br />
<br />
<p>We are very happy that the online information platform ‘biotechnologie.de’ recorded an interview with one of our participants, Charlotte Bunne, and included it into one of their weekly documentations recently. Initiated by the German ministry for education and science, <br />
<a href="http://www.biotechnologie.de/">biotechnologie.de</a><br />
is a public platform to inform not only scientists, but also people without scientific background about current results and technologies in biotechnological research. One of its main sections is a large video collection with short videos about various areas associated with biotechnology. The platform is constantly updated and revised and we are very glad to be in the latest online episode of biotechnologie.tv. Here, Charlotte explains briefly and very intelligible our iGEM HS project idea, the concept behind and what we are doing right now. You can watch the interview at the bottom of the page. It starts at 3:55 min.</p></br><br />
<br />
<iframe width="600px" height="400px" style="margin-left:20px;" src="http://www.youtube.com/embed/FxsJ8zEONTs" frameborder="0" allowfullscreen ><p>If the video doesn't appear here, please try reloading this page once or use the link below!</p></iframe></br></br><br />
<p> If the video doesn't appear here, please try reloading this page once or use the following link!</p><br />
<p>Link to the video: <a href="http://www.youtube.com/embed/FxsJ8zEONTs">http://www.youtube.com/embed/FxsJ8zEONTs</a></p><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T03:00:09Z<p>Dniopek: </p>
<hr />
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun, we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. <br />
<br/><br />
For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.<br />
We have worked with enthusiasm on the bacterial tool kit which can detect UV radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterization of different members of the same gene family as precA. Our assumption is that DNA damage leads to the successive activation of the different rec genes are activated dependent on the UV radiation dose. By implementing also these genes we want to generate a more gradual UV-response. The successive induction of the different rec promoters could help to determine the exact amount of radiation. This is important to provide the bacterial tool kit with the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and preC. PreB lead to a positive result, whereas induction of preC did not result in the generation of the reporter. Cloning of this part will be repeated. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not for sure whether precA is activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work because the recA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMS collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further detection systems for GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm which is the excitation wavelength of GFP. Therefore, we can make GFP visible using blue LEDs. Furthermore, we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. By using different fluorescent reporters we could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
The system still has to be optimized for real life application. Therefore, it has to be adapted to the sensitivity of mammalian cells towards UV-induced DNA damage. Among other things this would include optimization of the bacterial container which might absorb a certain amount of UV radiation. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people from too intense UV radiation but also are trendy accessories! <br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and to presenting our work to the other teams from all over the world. We hope to get into contact with other pupils interested in synthetic biology.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
<br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:59:09Z<p>Dniopek: </p>
<hr />
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. <br />
<br/><br />
For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.<br />
We have worked with enthusiasm on the bacterial tool kit which can detect UV radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterization of different members of the same gene family as precA. Our assumption is that DNA damage leads to the successive activation of the different rec genes are activated dependent on the UV radiation dose. By implementing also these genes we want to generate a more gradual UV-response. The successive induction of the different rec promoters could help to determine the exact amount of radiation. This is important to provide the bacterial tool kit with the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and preC. PreB lead to a positive result, whereas induction of preC did not result in the generation of the reporter. Cloning of this part will be repeated. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not for sure whether precA is activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work because the recA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMS collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further detection systems for GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm which is the excitation wavelength of GFP. Therefore, we can make GFP visible using blue LEDs. Furthermore, we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. By using different fluorescent reporters we could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
The system still has to be optimized for real life application. Therefore, it has to be adapted to the sensitivity of mammalian cells towards UV-induced DNA damage. Among other things this would include optimization of the bacterial container which might absorb a certain amount of UV radiation. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people from too intense UV radiation but also are trendy accessories! <br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and to presenting our work to the other teams from all over the world. We hope to get into contact with other pupils interested in synthetic biology.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
<br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:57:37Z<p>Dniopek: </p>
<hr />
<div>''Italic text''{{TopII}}<br />
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<br />
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. <br />
<br/><br />
For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.<br />
We have worked with enthusiasm on the bacterial tool kit which can detect UV radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterization of different members of the same gene family as precA. Our assumption is that DNA damage leads to the successive activation of the different rec genes are activated dependent on the UV radiation dose. By implementing also these genes we want to generate a more gradual UV-response. The successive induction of the different rec promoters could help to determine the exact amount of radiation. This is important to provide the bacterial tool kit with the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and preC. PreB lead to a positive result, whereas induction of preC did not result in the generation of the reporter. Cloning of this part will be repeated. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not for sure whether precA is activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work because the recA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMS collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further detection systems for GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm which is the excitation wavelength of GFP. Therefore, we can make GFP visible using blue LEDs. Furthermore, we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. By using different fluorescent reporters we could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
The system still has to be optimized for real life application. Therefore, it has to be adapted to the sensitivity of mammalian cells towards UV-induced DNA damage. Among other things this would include optimization of the bacterial container which might absorb a certain amount of UV radiation. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people from too intense UV radiation but also are trendy accessories! <br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
<br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:55:58Z<p>Dniopek: </p>
<hr />
<div>''Italic text''{{TopII}}<br />
{{Stylesheet}}<br />
<br />
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. <br />
<br/><br />
For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.<br />
We have worked with enthusiasm on the bacterial tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterization of different members of the same gene family as precA. Our assumption is that DNA damage leads to the successive activation of the different rec genes are activated dependent on the UV radiation dose. By implementing also these genes we want to generate a more gradual UV-response. The successive induction of the different rec promoters could help to determine the exact amount of radiation. This is important to provide the bacterial tool kit with the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and preC. PreB lead to a positive result, whereas induction of preC did not result in the generation of the reporter. Cloning of this part will be repeated. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not for sure whether precA is activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work because the recA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMS collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further detection systems for GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm which is the excitation wavelength of GFP. Therefore, we can make GFP visible using blue LEDs. Furthermore, we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. By using different fluorescent reporters we could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
The system still has to be optimized for real life application. Therefore, it has to be adapted to the sensitivity of mammalian cells towards UV-induced DNA damage. Among other things this would include optimization of the bacterial container which might absorb a certain amount of UV radiation. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people from too intense UV radiation but also are trendy accessories! <br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
<br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:55:18Z<p>Dniopek: </p>
<hr />
<div>''Italic text''{{TopII}}<br />
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<br />
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<div id="SubWrapper"><br />
<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. <br />
<br/><br />
For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.<br />
We have worked with enthusiasm on the bacterial tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterization of different members of the same gene family as precA. Our assumption is that DNA damage leads to the successive activation of the different rec genes are activated dependent on the UV radiation dose. By implementing also these genes we want to generate a more gradual UV-response. The successive induction of the different rec promoters could help to determine the exact amount of radiation. This is important to provide the bacterial tool kit with the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and preC. PreB lead to a positive result, whereas induction of preC did not result in the generation of the reporter. Cloning of this part will be repeated. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not for sure whether precA is activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work because the recA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMS collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further detection systems for GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm which is the excitation wavelength of GFP. Therefore, we can make GFP visible using blue LEDs. Furthermore, we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. By using different fluorescent reporters we could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
The system still has to be optimized for real life application. Therefore, it has to be adapted to the sensitivity of mammalian cells towards UV-induced DNA damage. Among other things this would include optimization of the bacterial container which might absorb a certain amount of UV radiation. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people from too intense UV radiation but also are trendy accessories! <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:52:48Z<p>Dniopek: </p>
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
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</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. <br />
<br/><br />
For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.<br />
We have worked with enthusiasm on the bacterial tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterization of different members of the same gene family as precA. Our assumption is that DNA damage leads to the successive activation of the different rec genes are activated dependent on the UV radiation dose. By implementing also these genes we want to generate a more gradual UV-response. The successive induction of the different rec promoters could help to determine the exact amount of radiation. This is important to provide the bacterial tool kit with the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and preC. PreB lead to a positive result, whereas induction of preC did not result in the generation of the reporter. Cloning of this part will be repeated. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not for sure whether precA is activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work because the recA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMS collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further detection systems for GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm which is the excitation wavelength of GFP. Therefore, we can make GFP visible using blue LEDs. Furthermore, we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. By using different fluorescent reporters we could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
The system still has to be optimized for real life application. Therefore, it has to be adapted to the sensitivity of mammalian cells towards UV-induced DNA damage. Among other things this would include optimization of the bacterial container which might absorb a certain amount of UV radiation. <br />
<br/><br />
At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Check_outTeam:Heidelberg LSL/Check out2012-06-17T02:50:00Z<p>Dniopek: </p>
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<h2>Check out</h2><br />
<p> Congratulations! You made your choice for one of our remarkable <b>iGEMS</b> products. Below, you can pre-order the chosen item simply by leaving your email address and a short text describing why the product would be most suitable especially for you...<br />
<br><br />
... ah... actually... this is not really true. The product you just tried to buy is not on the marked yet. Not even close. This online-shop is just a fake store and not real. But why would we present you a fake online-store you might ask? <br><br><br />
Well, the products we present here are embedded into the spheres of synthetic biology. Synthetic biology is a new approach in modern life-sciences which wants to make biology easier to engineer. It tries to adress problems in the fields of medicine, environment, energy and many more and aims for finding new solutions by e.g. developing new therapies or working on approaches to environmental-friendly energy. We hope that our online-shopping experience raised curiousity about this new scientific field of synthetic biology and we would be very happy to provide you with more information about it.<br />
<br />
Therefor, we would like draw your attention to the following pages to get informed about the theme: </p><br />
<ul><br />
<li><a href="https://igem.org/Main_Page">iGEM</a></li><br />
<li><a href="http://partsregistry.org/Main_Page">parts registry</a></li><br />
<li><a href="http://biobricks.org/">BioBricks Foundation</a></li><br />
<li><a href="http://syntheticbiology.org/">SyntheticBiology.org</a></li><br />
</ul><br />
<p>In addition, you can get more information about the purpose of the online store in our <a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Project_humanPractice">Human Practice</a> section. Furthermore, you can also use the contact form below in order to contact us directly. We would be happy to get in touch with you and provide you with more information about our project or synthetic biology in general. Last but not least, we are also happy about any comments about the fake-products in our little store and about your ideas on how to communicate synthetic biology to a broader public. Therefore, we are looking forward to receive your messages. <br><br><br />
<b>the iGEM Team Heidelberg LSL </b> </p><br />
<br />
<br />
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<ul class="blue"><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" title="Online_store" ><span>Online Store</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundra_col" title="Sundra_col" ><span>Sundra Collection</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundro_col" title="Sundro_col"><span>Sundro Collection</span></a></li><br />
</li></br></br></br></br></br></br><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Check_out" title="Check_out" class="current"><span>Check Out</span></a></li><br />
<img src="https://static.igem.org/mediawiki/2012hs/8/81/Einkaufswagen-2.png" width="40"/><br />
</ul><br />
</div><br />
<br />
<br />
<center><br />
<div id="box-expl" ><br />
<p > All iGEMS are equipped with a flask containing an UV-inducible <i>E. coli</i> solution. Click <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project">here</a> to learn more about our project.</p><br />
</div><!--end boxIdea--><br />
<br />
<div id = "box-3"><br />
<img id="pic2" src="https://static.igem.org/mediawiki/2012hs/8/83/Eppi.png" width="250"/><br />
</div><br />
<br />
</center><br />
</div><!--end news--><br />
</div><!-- end super_main_wrapper--><br />
</body><br />
<br />
<br />
<script type="text/javascript"><br />
function setPageSize() {<br />
len = document.getElementById('super_main_wrapper').offsetHeight;<br />
document.getElementById('bodyContent').style.height = len + 'px';<br />
document.getElementById('SupWrapper').style.height = len + 'px';<br />
document.getElementById('news').style.height = len + 'px';<br />
}<br />
document.forms["InputForm"].elements["ctrl_9"].value = getValue("name");<br />
</script><br />
</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Check_outTeam:Heidelberg LSL/Check out2012-06-17T02:49:16Z<p>Dniopek: </p>
<hr />
<div>{{TopII}}<br />
{{Stylesheet}}<br />
{{SideBar}}<br />
<br />
<html><br />
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<style type="text/css"><br />
#news {min-height: 1000px;}<br />
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case "SurfN": url_curr = 'https://static.igem.org/mediawiki/2012hs/f/f3/SurfN.png';<br />
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<br />
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</script><br />
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<body id="home" onload="setPageSize()"><br />
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<br />
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document.write(url);<br />
document.write("' /></center>");<br />
</script><br />
<br />
<br />
<h2>Check out</h2><br />
<p> Congratulations! You made your choice for one of our remarkable <b>iGEMS</b> products. Below, you can pre-order the chosen item simply by leaving your email address and a short text describing why the product would be most suitable especially for you...<br />
<br><br />
... ah... actually... this is not really true. The product you just tried to buy is not on the marked yet. Not even close. This online-shop is just a fake store and not real. But why would we present you a fake online-store you might ask? <br><br><br />
Well, the products we present here are embedded into the spheres of synthetic biology. Synthetic biology is a new approach in modern life-sciences which wants to make biology easier to engineer. It tries to adress problems in the fields of medicine, environment, energy and many more and aims for finding new solutions by e.g. developing new therapies or working on approaches to environmental-friendly energy. We hope that our online-shopping experience raised curiousity about this new scientific field of synthetic biology and we would be very happy to provide you with more information about it.<br />
<br />
Therefor, we would like draw your attention to the following pages to get informed about the theme: </p><br />
<ul><br />
<li><a href="https://igem.org/Main_Page">iGEM</a></li><br />
<li><a href="http://partsregistry.org/Main_Page">parts registry</a></li><br />
<li><a href="http://biobricks.org/">BioBricks Foundation</a></li><br />
<li><a href="http://syntheticbiology.org/">SyntheticBiology.org</a></li><br />
</ul><br />
<p>In addition, you can get more information about the purpose of the online store in our <a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Project_humanPractice">Human Practice</a> section. Furthermore, you can also use the contact form below in order to contact us directly. We would be happy to get in touch with you and provide you with more information about our project or synthetic biology in general. Last but not least, we are also happy about any comments about the fake-products in our little store and about your ideas on how to communicate synthetic biology to a broader public. Therefore, we are looking forward to receive your messages. <br><br><br />
<b>With the best wishes- the students of the iGEM Team Heidelberg LSL </b> </p><br />
<br />
<br />
<br />
<br />
<form id="InputForm" action="mailto:synbio.lsl@googlemail.com " method="post" enctype="text/plain" ><br />
</br></br><br />
<table><br />
<tr class="row_0 row_first even"><br />
<td class="col_0 col_first"><label for="ctrl_7" class="mandatory"><span class="invisible"></span> Name:<span class="mandatory">*</span></label></td><br />
<td class="col_1 col_last"><input type="text" name="Name " id="ctrl_7" class="text mandatory" value="" maxlength="100" /></td><br />
</tr><br />
<tr class="row_1 odd"><br />
<td class="col_0 col_first"><label for="ctrl_8" class="mandatory"><span class="invisible"></span> Subject:<span class="mandatory">*</span></label></td><br />
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<td class="col_1 col_last"><input type="text" name="Item " id="ctrl_9" class="text mandatory" value="" maxlength="100" /></td><br />
</tr><br />
<tr class="row_3 odd"><br />
<td class="col_0 col_first"><label for="ctrl_9" class="mandatory"><span class="invisible"></span> Message:<span class="mandatory">*</span></label></td><br />
<td class="col_1 col_last"><textarea name="Message " id="ctrl_10" class="textarea mandatory" rows="15" cols="60"></textarea></td><br />
</tr><br />
<br />
<tr class="row_5 row_last odd"><br />
<td class="col_0 col_first">&nbsp;</td><br />
<td class="col_1 col_last"><div class="submit_container"><input type="submit" id="ctrl_11" class="submit" value="Submit" /></div></td><br />
</tr><br />
</table><br />
</form><br />
</div><!--EndSubWrapper--><br />
<br />
<div id="news"> <br />
<div id="Shop_menu" ><br />
<ul class="blue"><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" title="Online_store" ><span>Online Store</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundra_col" title="Sundra_col" ><span>Sundra Collection</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundro_col" title="Sundro_col"><span>Sundro Collection</span></a></li><br />
</li></br></br></br></br></br></br><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Check_out" title="Check_out" class="current"><span>Check Out</span></a></li><br />
<img src="https://static.igem.org/mediawiki/2012hs/8/81/Einkaufswagen-2.png" width="40"/><br />
</ul><br />
</div><br />
<br />
<br />
<center><br />
<div id="box-expl" ><br />
<p > All iGEMS are equipped with a flask containing an UV-inducible <i>E. coli</i> solution. Click <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project">here</a> to learn more about our project.</p><br />
</div><!--end boxIdea--><br />
<br />
<div id = "box-3"><br />
<img id="pic2" src="https://static.igem.org/mediawiki/2012hs/8/83/Eppi.png" width="250"/><br />
</div><br />
<br />
</center><br />
</div><!--end news--><br />
</div><!-- end super_main_wrapper--><br />
</body><br />
<br />
<br />
<script type="text/javascript"><br />
function setPageSize() {<br />
len = document.getElementById('super_main_wrapper').offsetHeight;<br />
document.getElementById('bodyContent').style.height = len + 'px';<br />
document.getElementById('SupWrapper').style.height = len + 'px';<br />
document.getElementById('news').style.height = len + 'px';<br />
}<br />
document.forms["InputForm"].elements["ctrl_9"].value = getValue("name");<br />
</script><br />
</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Check_outTeam:Heidelberg LSL/Check out2012-06-17T02:48:28Z<p>Dniopek: </p>
<hr />
<div>{{TopII}}<br />
{{Stylesheet}}<br />
{{SideBar}}<br />
<br />
<html><br />
<br />
<style type="text/css"><br />
#news {min-height: 1000px;}<br />
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<br />
#box-3 {position: absolute;<br />
z-index:1;<br />
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<br />
</style><br />
<br />
<script type="text/javascript"><br />
function getValue(varname)<br />
{<br />
// First, we load the URL into a variable<br />
var url = window.location.href;<br />
<br />
// Next, split the url by the ?<br />
var qparts = url.split("?");<br />
<br />
// Check that there is a querystring, return "" if not<br />
if (qparts.length == 0)<br />
{<br />
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<br />
// Then find the querystring, everything after the ?<br />
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<br />
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var vars = query.split("&");<br />
<br />
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// Iterate through vars, checking each one for varname<br />
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<br />
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<br />
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}<br />
<br />
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<br />
var url_curr = "";<br />
<br />
switch (varname) {<br />
<br />
case "SunnyB": url_curr = 'https://static.igem.org/mediawiki/2012hs/f/fd/SunnyB.png';<br />
break;<br />
<br />
case "PinkS": url_curr = 'https://static.igem.org/mediawiki/2012hs/9/93/PinkS.png';<br />
break; <br />
<br />
case "SummerR": url_curr = 'https://static.igem.org/mediawiki/2012hs/9/9c/SummerR.png';<br />
break;<br />
<br />
case "SurfN": url_curr = 'https://static.igem.org/mediawiki/2012hs/f/f3/SurfN.png';<br />
break;<br />
<br />
case "IcyF": url_curr = 'https://static.igem.org/mediawiki/2012hs/7/74/IcyF.png';<br />
break; <br />
}<br />
<br />
return url_curr;<br />
<br />
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</script><br />
<br />
<body id="home" onload="setPageSize()"><br />
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<br />
<br />
<div id="super_main_wrapper"><br />
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<br />
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<br />
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<br />
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document.write(url);<br />
document.write("' /></center>");<br />
</script><br />
<br />
<br />
<h2>Check out</h2><br />
<p> Congratulations! You made your choice for one of our remarkable <b>iGEMS</b> products. Below, you can pre-order the chosen item simply by leaving your email address and a short text describing why the product would be most suitable especially for you...<br />
<br><br />
... ah... actually... this is not really true. The product you just tried to buy is not on the marked yet. Not even close. This online-shop is just a fake store and not real. But why would we present you a fake online-store you might ask? <br><br><br />
Well, the products we present here are embedded into the spheres of synthetic biology. Synthetic biology is a new approach in modern life-sciences which wants to make biology easier to engineer. It tries to adress problems in the fields of medicine, environment, energy and many more and aims for finding new solutions by e.g. developing new therapies or working on approaches to environmental-friendly energy. We hope that our online-shopping experience raised curiousity about this new scientific field of synthetic biology and we would be very happy to provide you with more information about it.<br />
<br />
Therefor, we would like draw your attention to the following pages to get informed about the theme: </p><br />
<ul><br />
<li><a href="https://igem.org/Main_Page">iGEM</a></li><br />
<li><a href="http://partsregistry.org/Main_Page">parts registry</a></li><br />
<li><a href="http://biobricks.org/">BioBricks Foundation</a></li><br />
<li><a href="http://syntheticbiology.org/">SyntheticBiology.org</a></li><br />
</ul><br />
<p>In addition, you can get more information about the purpose of the online store in our <a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Project_humanPractice">Human Practice</a> section. Furthermore, you can also use the contact form below in order to contact us directly. We would be happy to get in touch with you and provide you with more information about our project or synthetic biology in general. Last but not least, we are also happy about any comments about the fake-products in our little store and about your ideas on how to community synthetic biology to a broader public. Therefore, we are looking forward to receive your messages. <br><br><br />
<b>With the best wishes- the students of the iGEM Team Heidelberg LSL </b> </p><br />
<br />
<br />
<br />
<br />
<form id="InputForm" action="mailto:synbio.lsl@googlemail.com " method="post" enctype="text/plain" ><br />
</br></br><br />
<table><br />
<tr class="row_0 row_first even"><br />
<td class="col_0 col_first"><label for="ctrl_7" class="mandatory"><span class="invisible"></span> Name:<span class="mandatory">*</span></label></td><br />
<td class="col_1 col_last"><input type="text" name="Name " id="ctrl_7" class="text mandatory" value="" maxlength="100" /></td><br />
</tr><br />
<tr class="row_1 odd"><br />
<td class="col_0 col_first"><label for="ctrl_8" class="mandatory"><span class="invisible"></span> Subject:<span class="mandatory">*</span></label></td><br />
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<td class="col_1 col_last"><input type="text" name="Item " id="ctrl_9" class="text mandatory" value="" maxlength="100" /></td><br />
</tr><br />
<tr class="row_3 odd"><br />
<td class="col_0 col_first"><label for="ctrl_9" class="mandatory"><span class="invisible"></span> Message:<span class="mandatory">*</span></label></td><br />
<td class="col_1 col_last"><textarea name="Message " id="ctrl_10" class="textarea mandatory" rows="15" cols="60"></textarea></td><br />
</tr><br />
<br />
<tr class="row_5 row_last odd"><br />
<td class="col_0 col_first">&nbsp;</td><br />
<td class="col_1 col_last"><div class="submit_container"><input type="submit" id="ctrl_11" class="submit" value="Submit" /></div></td><br />
</tr><br />
</table><br />
</form><br />
</div><!--EndSubWrapper--><br />
<br />
<div id="news"> <br />
<div id="Shop_menu" ><br />
<ul class="blue"><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" title="Online_store" ><span>Online Store</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundra_col" title="Sundra_col" ><span>Sundra Collection</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundro_col" title="Sundro_col"><span>Sundro Collection</span></a></li><br />
</li></br></br></br></br></br></br><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Check_out" title="Check_out" class="current"><span>Check Out</span></a></li><br />
<img src="https://static.igem.org/mediawiki/2012hs/8/81/Einkaufswagen-2.png" width="40"/><br />
</ul><br />
</div><br />
<br />
<br />
<center><br />
<div id="box-expl" ><br />
<p > All iGEMS are equipped with a flask containing an UV-inducible <i>E. coli</i> solution. Click <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project">here</a> to learn more about our project.</p><br />
</div><!--end boxIdea--><br />
<br />
<div id = "box-3"><br />
<img id="pic2" src="https://static.igem.org/mediawiki/2012hs/8/83/Eppi.png" width="250"/><br />
</div><br />
<br />
</center><br />
</div><!--end news--><br />
</div><!-- end super_main_wrapper--><br />
</body><br />
<br />
<br />
<script type="text/javascript"><br />
function setPageSize() {<br />
len = document.getElementById('super_main_wrapper').offsetHeight;<br />
document.getElementById('bodyContent').style.height = len + 'px';<br />
document.getElementById('SupWrapper').style.height = len + 'px';<br />
document.getElementById('news').style.height = len + 'px';<br />
}<br />
document.forms["InputForm"].elements["ctrl_9"].value = getValue("name");<br />
</script><br />
</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Check_outTeam:Heidelberg LSL/Check out2012-06-17T02:47:43Z<p>Dniopek: </p>
<hr />
<div>{{TopII}}<br />
{{Stylesheet}}<br />
{{SideBar}}<br />
<br />
<html><br />
<br />
<style type="text/css"><br />
#news {min-height: 1000px;}<br />
<br />
<br />
#box-3 {position: absolute;<br />
z-index:1;<br />
top:300px;<br />
right:100px;<br />
width:180px;<br />
height: 260px;<br />
}<br />
<br />
#box-expl{<br />
position: absolute;<br />
z-index:3;<br />
top:430px;<br />
right:65px;<br />
width:200px;<br />
}<br />
<br />
#InputForm{<br />
position:absolute;<br />
left:20px;<br />
}<br />
<br />
ul{<br />
margin-left:20px;<br />
padding-left:50px;<br />
}<br />
<br />
</style><br />
<br />
<script type="text/javascript"><br />
function getValue(varname)<br />
{<br />
// First, we load the URL into a variable<br />
var url = window.location.href;<br />
<br />
// Next, split the url by the ?<br />
var qparts = url.split("?");<br />
<br />
// Check that there is a querystring, return "" if not<br />
if (qparts.length == 0)<br />
{<br />
return "";<br />
}<br />
<br />
// Then find the querystring, everything after the ?<br />
var query = qparts[1];<br />
<br />
// Split the query string into variables (separates by &s)<br />
var vars = query.split("&");<br />
<br />
// Initialize the value with "" as default<br />
var value = "";<br />
<br />
// Iterate through vars, checking each one for varname<br />
for (i=0;i<vars.length;i++)<br />
{<br />
// Split the variable by =, which splits name and value<br />
var parts = vars[i].split("=");<br />
<br />
// Check if the correct variable<br />
if (parts[0] == varname)<br />
{<br />
// Load value into variable<br />
value = parts[1];<br />
<br />
// End the loop<br />
break;<br />
}<br />
}<br />
<br />
// Convert escape code<br />
value = unescape(value);<br />
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<br />
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break;<br />
<br />
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break; <br />
<br />
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break;<br />
<br />
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break;<br />
<br />
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document.write(url);<br />
document.write("' /></center>");<br />
</script><br />
<br />
<br />
<h2>Check out</h2><br />
<p> Congratulations! You made your choice for one of our remarkable <b>iGEMS</b> products. Below, you can pre-order the chosen item simply by leaving your email address and a short text describing why the product would be most suitable especially for you...<br />
<br><br />
... ah... actually... this is not really true. The product you just tried to buy is not on the marked yet. Not even close. This online-shop is just a fake store and not real. But why would we present you a fake online-store you might ask? <br><br><br />
Well, the products we present here are embedded into the spheres of synthetic biology. Synthetic biology is a new approach in modern life-sciences which wants to make biology easier to engineer. It tries to adress problems in the fields of medicine, environment, energy and many more and aims for finding new solutions by e.g. developing new therapies or working on approaches to environmental-friendly energy. We hope that our online-shopping experience raised curiousity about this new scientific field of synthetic biology and we would be very happy to provide you with more information about it.<br />
<br />
Therefor, we would like draw your attention to the following pages to get informed about the theme: </p><br />
<ul><br />
<li><a href="https://igem.org/Main_Page">iGEM</a></li><br />
<li><a href="http://partsregistry.org/Main_Page">parts registry</a></li><br />
<li><a href="http://biobricks.org/">BioBricks Foundation</a></li><br />
<li><a href="http://syntheticbiology.org/">SyntheticBiology.org</a></li><br />
</ul><br />
<p>In addition, you can get more information about the purpose of the Online Store in our <a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Project_humanPractice">Human Practice</a> section. Furthermore, you can also use the contact form below in order to contact us directly. We would be happy to get in touch with you and provide you with more information about our project or synthetic biology in general. Last but not least, we are also happy about any comments about the fake-products in our little store and about your ideas on how to community synthetic biology to a broader public. Therefore, we are looking forward to receive your messages. <br><br><br />
<b>With the best wishes- the students of the iGEM Team Heidelberg LSL </b> </p><br />
<br />
<br />
<br />
<br />
<form id="InputForm" action="mailto:synbio.lsl@googlemail.com " method="post" enctype="text/plain" ><br />
</br></br><br />
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<td class="col_0 col_first"><label for="ctrl_7" class="mandatory"><span class="invisible"></span> Name:<span class="mandatory">*</span></label></td><br />
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<td class="col_0 col_first"><label for="ctrl_8" class="mandatory"><span class="invisible"></span> Subject:<span class="mandatory">*</span></label></td><br />
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<td class="col_0 col_first"><label for="ctrl_8" class="mandatory"><span class="invisible"></span> Item:<span class="mandatory">*</span></label></td><br />
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<br />
<div id="news"> <br />
<div id="Shop_menu" ><br />
<ul class="blue"><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" title="Online_store" ><span>Online Store</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundra_col" title="Sundra_col" ><span>Sundra Collection</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundro_col" title="Sundro_col"><span>Sundro Collection</span></a></li><br />
</li></br></br></br></br></br></br><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Check_out" title="Check_out" class="current"><span>Check Out</span></a></li><br />
<img src="https://static.igem.org/mediawiki/2012hs/8/81/Einkaufswagen-2.png" width="40"/><br />
</ul><br />
</div><br />
<br />
<br />
<center><br />
<div id="box-expl" ><br />
<p > All iGEMS are equipped with a flask containing an UV-inducible <i>E. coli</i> solution. Click <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project">here</a> to learn more about our project.</p><br />
</div><!--end boxIdea--><br />
<br />
<div id = "box-3"><br />
<img id="pic2" src="https://static.igem.org/mediawiki/2012hs/8/83/Eppi.png" width="250"/><br />
</div><br />
<br />
</center><br />
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document.forms["InputForm"].elements["ctrl_9"].value = getValue("name");<br />
</script><br />
</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Check_outTeam:Heidelberg LSL/Check out2012-06-17T02:47:07Z<p>Dniopek: </p>
<hr />
<div>{{TopII}}<br />
{{Stylesheet}}<br />
{{SideBar}}<br />
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<br />
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<br />
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<br />
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document.write(url);<br />
document.write("' /></center>");<br />
</script><br />
<br />
<br />
<h2>Check out</h2><br />
<p> Congratulations! You made your choice for one of our remarkable <b>iGEMS</b> products. Below, you can pre-order the chosen item simply by leaving your email address and a short text describing why the product would be most suitable especially for you...<br />
<br><br />
... ah... actually... this is not really true. The product you just tried to buy is not on the marked yet. Not even close. This online-shop is just a fake store and not real. But why would we present you a fake online-store you might ask? <br><br />
Well, the products we present here are embedded into the spheres of synthetic biology. Synthetic biology is a new approach in modern life-sciences which wants to make biology easier to engineer. It tries to adress problems in the fields of medicine, environment, energy and many more and aims for finding new solutions by e.g. developing new therapies or working on approaches to environmental-friendly energy. We hope that our online-shopping experience raised curiousity about this new scientific field of synthetic biology and we would be very happy to provide you with more information about it.<br />
<br />
Therefor, we would like draw your attention to the following pages to get informed about the theme: </p><br />
<ul><br />
<li><a href="https://igem.org/Main_Page">iGEM</a></li><br />
<li><a href="http://partsregistry.org/Main_Page">parts registry</a></li><br />
<li><a href="http://biobricks.org/">BioBricks Foundation</a></li><br />
<li><a href="http://syntheticbiology.org/">SyntheticBiology.org</a></li><br />
</ul><br />
<p>In addition, you can get more information about the purpose of the Online Store in our <a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Project_humanPractice">Human Practice</a> section. Furthermore, you can also use the contact form below in order to contact us directly. We would be happy to get in touch with you and provide you with more information about our project or synthetic biology in general. Last but not least, we are also happy about any comments about the fake-products in our little store and about your ideas on how to community synthetic biology to a broader public. Therefore, we are looking forward to receive your messages. <br><br><br />
<b>With the best wishes- the students of the iGEM Team Heidelberg LSL </b> </p><br />
<br />
<br />
<br />
<br />
<form id="InputForm" action="mailto:synbio.lsl@googlemail.com " method="post" enctype="text/plain" ><br />
</br></br><br />
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<td class="col_0 col_first"><label for="ctrl_7" class="mandatory"><span class="invisible"></span> Name:<span class="mandatory">*</span></label></td><br />
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<br />
<div id="news"> <br />
<div id="Shop_menu" ><br />
<ul class="blue"><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" title="Online_store" ><span>Online Store</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundra_col" title="Sundra_col" ><span>Sundra Collection</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundro_col" title="Sundro_col"><span>Sundro Collection</span></a></li><br />
</li></br></br></br></br></br></br><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Check_out" title="Check_out" class="current"><span>Check Out</span></a></li><br />
<img src="https://static.igem.org/mediawiki/2012hs/8/81/Einkaufswagen-2.png" width="40"/><br />
</ul><br />
</div><br />
<br />
<br />
<center><br />
<div id="box-expl" ><br />
<p > All iGEMS are equipped with a flask containing an UV-inducible <i>E. coli</i> solution. Click <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project">here</a> to learn more about our project.</p><br />
</div><!--end boxIdea--><br />
<br />
<div id = "box-3"><br />
<img id="pic2" src="https://static.igem.org/mediawiki/2012hs/8/83/Eppi.png" width="250"/><br />
</div><br />
<br />
</center><br />
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document.forms["InputForm"].elements["ctrl_9"].value = getValue("name");<br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:46:31Z<p>Dniopek: </p>
<hr />
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. <br />
<br/><br />
For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.<br />
We have worked with enthusiasm on the bacterial tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterization of different members of the same gene family as precA. Our assumption is that DNA damage leads to the successive activation of the different rec genes are activated dependent on the UV radiation dose. By implementing also these genes we want to generate a more gradual UV-response. The successive induction of the different rec promoters could help to determine the exact amount of radiation. This is important to provide the bacterial tool kit with the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and preC. PreB lead to a positive result, whereas induction of preC did not result in the generation of the reporter. Cloning of this part will be repeated. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not for sure whether precA is activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work because the recA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMS collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further detection systems for GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm which is the excitation wavelength of GFP. Therefore, we can make GFP visible using blue LEDs. Furthermore, we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. By using different fluorescent reporters we could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
For the production of the jewelry, which shall be once a common product for the public, we need to simplify this system. Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue, when it is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way to recognize UV-radiation, which does not need any further background in synthetic biology. At the moment our bacteria turn blue after 10 min exposure. But the human body can resist much more UV-light without getting DNA-damage. We have to find a rec gene that show the most similarities with the human body. An other possibility to handle this problem would be to vary the UV permeability of the bacteria containment.<br />
<br/><br />
<br />
Some more problems that came out while working on the constructs. At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Check_outTeam:Heidelberg LSL/Check out2012-06-17T02:45:53Z<p>Dniopek: </p>
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<br />
<h2>Check out</h2><br />
<p> Congratulations! You made your choice for one of our remarkable <b>iGEMS</b> products. Below, you can pre-order the chosen item simply by leaving your email address and a short text describing why the product would be most suitable especially for you...<br />
<br><br />
... ah... actually... this is not really true. The product you just tried to buy is not on the marked yet. Not even close. But why would we present you a fake online-store you might ask? <br><br />
Well, the products we present here are embedded into the spheres of synthetic biology. Synthetic biology is a new approach in modern life-sciences which wants to make biology easier to engineer. It tries to adress problems in the fields of medicine, environment, energy and many more and aims for finding new solutions by e.g. developing new therapies or working on approaches to environmental-friendly energy. We hope that our online-shopping experience raised curiousity about this new scientific field of synthetic biology and we would be very happy to provide you with more information about it.<br />
<br />
Therefor, we would like draw your attention to the following pages to get informed about the theme: </p><br />
<ul><br />
<li><a href="https://igem.org/Main_Page">iGEM</a></li><br />
<li><a href="http://partsregistry.org/Main_Page">parts registry</a></li><br />
<li><a href="http://biobricks.org/">BioBricks Foundation</a></li><br />
<li><a href="http://syntheticbiology.org/">SyntheticBiology.org</a></li><br />
</ul><br />
<p>In addition, you can get more information about the purpose of the Online Store in our <a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Project_humanPractice">Human Practice</a> section. Furthermore, you can also use the contact form below in order to contact us directly. We would be happy to get in touch with you and provide you with more information about our project or synthetic biology in general. Last but not least, we are also happy about any comments about the fake-products in our little store and about your ideas on how to community synthetic biology to a broader public. Therefore, we are looking forward to receive your messages. <br><br><br />
<b>With the best wishes- the students of the iGEM Team Heidelberg LSL </b> </p><br />
<br />
<br />
<br />
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<ul class="blue"><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" title="Online_store" ><span>Online Store</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundra_col" title="Sundra_col" ><span>Sundra Collection</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundro_col" title="Sundro_col"><span>Sundro Collection</span></a></li><br />
</li></br></br></br></br></br></br><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Check_out" title="Check_out" class="current"><span>Check Out</span></a></li><br />
<img src="https://static.igem.org/mediawiki/2012hs/8/81/Einkaufswagen-2.png" width="40"/><br />
</ul><br />
</div><br />
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<p > All iGEMS are equipped with a flask containing an UV-inducible <i>E. coli</i> solution. Click <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project">here</a> to learn more about our project.</p><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:41:03Z<p>Dniopek: </p>
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
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<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun exposure than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. <br />
<br/><br />
For us, personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But, nevertheless, we have encountered difficulties that could be optimized in the future.<br />
We have worked with enthusiasm on the bacterial tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterization of different members of the same gene family as precA. Our assumption is that DNA damage leads to the successive activation of the different rec genes are activated dependent on the UV radiation dose. By implementing also these genes we want to generate a more gradual UV-response. The successive induction of the different rec promoters could help to determine the exact amount of radiation. This is important to provide the bacterial tool kit with the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and preC. PreB lead to a positive result, whereas induction of preC did not result in the generation of the reporter. Cloning of this part will be repeated. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analyzed whether the construct would also work with radioactive radiation. Radioactive radiation causes DNA damage different from UV-induced DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMs collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further measurement systems to detect GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm. Therefore we can make GFP visible using blue LEDs. Furthermore we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. We could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
For the production of the jewelry, which shall be once a common product for the public, we need to simplify this system. Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue, when it is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way to recognize UV-radiation, which does not need any further background in synthetic biology. At the moment our bacteria turn blue after 10 min exposure. But the human body can resist much more UV-light without getting DNA-damage. We have to find a rec gene that show the most similarities with the human body. An other possibility to handle this problem would be to vary the UV permeability of the bacteria containment.<br />
<br/><br />
<br />
Some more problems that came out while working on the constructs. At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Check_outTeam:Heidelberg LSL/Check out2012-06-17T02:38:05Z<p>Dniopek: </p>
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<h2>Check out</h2><br />
<p> Congratulations! You made your choice for one of our remarkable <b>iGEMS</b> products. Below, you can pre-order the chosen item simply by leaving your email address and a short text describing why the product would be most suitable especially for you...<br />
<br><br />
... ah... actually... this is not really true. The product you just tried to buy is not on the marked yet. Not even close. But why would we present you a fake online-store you might ask? <br />
Well, the products we present here are embedded into the spheres of synthetic biology. Synthetic biology is a new approach in modern life-sciences which aims for making biology easier to engineer. It tries to adress problems in the fields of medicine, environment, energy and more and find new solutions by e.g. developing new therapies or new working on approaches to environmental-friendly energy. If our online-shop expierence raised curiousity about this upcoming and new scientific field of synthetic biology, we would be very happy to provide you with more information.<br />
<br />
Therefor, we would like draw your attention to the following pages to get informed about the theme: </p><br />
<ul><br />
<li><a href="https://igem.org/Main_Page">iGEM</a></li><br />
<li><a href="http://partsregistry.org/Main_Page">parts registry</a></li><br />
<li><a href="http://biobricks.org/">BioBricks Foundation</a></li><br />
</ul><br />
<p>Read more about the purpose of the Online Store in our in the <a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Project_humanPractice">Human Practice</a> section.</p><br />
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<ul class="blue"><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" title="Online_store" ><span>Online Store</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundra_col" title="Sundra_col" ><span>Sundra Collection</span></a></li><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Sundro_col" title="Sundro_col"><span>Sundro Collection</span></a></li><br />
</li></br></br></br></br></br></br><br />
<li><a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Check_out" title="Check_out" class="current"><span>Check Out</span></a></li><br />
<img src="https://static.igem.org/mediawiki/2012hs/8/81/Einkaufswagen-2.png" width="40"/><br />
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<p > All iGEMS are equipped with a flask containing an UV-inducible <i>E. coli</i> solution. Click <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Project">here</a> to learn more about our project.</p><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:26:28Z<p>Dniopek: </p>
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
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<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun expose than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern context and give people a better understanding of synthetic biology. <br />
<br/><br />
For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But furthermore we have found difficulties that should be optimised in the future.<br />
We have worked with enthusiasm on the bacteria tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterisation of different members of the same gene family as precA. So Our assumption is that different rec genes are activated based on a certain characteristic extension of DNA damage dependent on UV radiation. By linking these genes together we want to generate a more gradual UV-response for the detection of qualitative UV-intensity. At UV-intensities different genes are activated and a color gradient appears. This gradient will be used to determine the exact amount of radiation. This is important to give the bacteria tool kit the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and precC, they already gave us a positive result, so that our assumption was right. Now we have to analyse the data to find their most effective use. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analysed if the construct would also work with radioactive radiation. Radioactive radiation causes different DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMs collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further measurement systems to detect GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm. Therefore we can make GFP visible using blue LEDs. Furthermore we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. We could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
For the production of the jewelry, which shall be once a common product for the public, we need to simplify this system. Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue, when it is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way to recognize UV-radiation, which does not need any further background in synthetic biology. At the moment our bacteria turn blue after 10 min exposure. But the human body can resist much more UV-light without getting DNA-damage. We have to find a rec gene that show the most similarities with the human body. An other possibility to handle this problem would be to vary the UV permeability of the bacteria containment.<br />
<br/><br />
<br />
Some more problems that came out while working on the constructs. At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
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<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
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<h2>Human Practice</h2><br />
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As the Heidelberg Life-Science Lab is a place where science meets interested pupils, for us, it is very important to draw the connection between our scientific work and the non-scientific community. Many people are not aware of synthetic biology and only a minority is really informed about its potential. With our work, we want to encourage non-scientific readers to negotiate possible prejudices, learn more about our work in the lab and have an entertaining insight into the various applications of our new biological system. <br />
<br />
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<h4>How can high-school students learn about genetic engineering?</h4><br />
<br />
<br />
<p>Learning by heart belongs to the past. We are learning by doing. Being normal high-school students, we want to show that everybody can understand the basic principles of how genetic circuits in cells can be 'engineered' and how intelligible most of the methods are. With our many explanations and protocols, we want to support, encourage and introduce upcoming high-school teams, interested teenagers, students and adults without vast knowledge to get involved into synthetic and molecular biology.</p><br />
<br />
<br />
<h4>Science interesting and intelligible - why not writing a book?</h4><br />
<br />
<p>This is the idea and motivation of the popular german scientific writer Dr. Olaf Fritsche. His upcoming book deals with the future of biological sciences. He will explain how scientific knowledge arises and show that everybody can understand facts when asking the right questions in science. Olaf approached us some weeks ago and asked whether he could visit our iGEM HS team in order to ask some basic questions about our project and team. Finally, he did not only visit us once, but several times, both inside and outside of the laboratory and followed our project from its start with the idea to its final end (his last visit was in the wiki-freeze night and he will follow up also after the iGEM jamboree). Its our great pleasure and honor, that Olaf Fritsche will report about our teams' and projects' development during taking part in the iGEM HS competition in his book in several passages embedded in different chapters. Fritsche’s work will show that establishing an iGEM project extracurricularly is ambitious but in the same way funny and intelligible. We hope that his book will encourage people outside of the synthetic biology community to overcome prejudices against science, in particular molecular biology and synthetic biology and become more educated and informed about modern life-sciences developments.</p><br />
<br />
<br />
<h4>Why iGEMs and an Online Shop?</h4><br />
<br />
<p>We carefully thought about possible applications of our UV-measuring system and decided that we should work in direction of an everyday-life product of which the general public should have most profits.<br />
For the integration of our measurment construct containing bacteria into such a product context, we thought about a funny and innovative way that could be coherent with current trends and that should evoke interest and curiosity amongst people. iGEMs, genetically modified gems, on bracelets and necklaces are a nice way to communicate our ideas to a great public.<br/><br />
<br />
The way we communicate our products is an online store <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Online_store">‘Online store’</a> which is - of course - not a real store, but just a fake (you can neither buy the iGEMS, nor the UV-sensing bacteria or anything). The store is supposed to give the user a reality-like impression of an everyday-life product which could be developed by using synthetic biology approaches. ´We tried to give it an authentic outfit in order to show that such a shop could probably become reality in the future. To overcome the distance to our product, we tried to imitate with our ‘Online Shop’ common structure and design that the user knows from other online-shops and stores. However, when the user gets interested in the product and wants to buy it, we redirect him to a <br />
<br />
Here, interested readers can pre book an article and should explain briefly why the product would be most suitable especially for them. We would like to use this information to get an impression of the wishes of interested people in order to optimize our products and to get better insight of the common favour. Doing so, we want to show that we are eager to communicate with the non-scientific public and to encourage them in particular to think about considering iGEMs as a general possible application of small genetically engineered machines. </p><br />
<br />
Our idea of monitoring the intensity of the UV-radiation mediated by the sun goes along with the efforts of the Heidelberg German Cancer Reasearch Center for cancer prevention. This is due to the fact that continuous high doses of UV-radiation are one major risc factor for melanoma development. One of our major goals is to achieve more general awareness of cancer risks and cancer prevention possibilities. In our case we want to make sunbathing teenagers more aware of the enhanced risk to develop malign melanoma (black skin cancer) by getting sunburned and being exposed to intense UV-radiation frequently.<br/><br />
<br />
<h4>Watch us on biotechnologie.tv! (in German language)</h4><br />
<br />
<p>We are very happy that the online information platform ‘biotechnologie.de’ involved an interview with one of our participants, Charlotte Bunne, in one of their short documentations. Initiated by the German ministry for education and science, <br />
<a href="http://www.biotechnologie.de/">biotechnologie.de</a><br />
is a public platform to inform not only scientists, but also people without scientific backround about current results and technologies in biotechnological research. One of its main rubrics is a large video collection with short videos about many various areas associated with biotechnology. The platform always is up to date and we are very glad to be in the latest episode of biotechnologie.tv. Here, Charlotte explains briefly and very intelligible our idea, our concept and what we are doing right now. You can watch the interview at the bottom of the page. It starts at 3:55 min.</p></br><br />
<br />
<iframe width="600px" height="400px" style="margin-left:20px;" src="http://www.youtube.com/embed/FxsJ8zEONTs" frameborder="0" allowfullscreen ><p>If the video doesn't appear here, please try reloading this page once or use the link below!</p></iframe></br></br><br />
<p> If the video doesn't appear here, please try reloading this page once or use the following link!</p><br />
<p>Link to the video: <a href="http://www.youtube.com/embed/FxsJ8zEONTs">http://www.youtube.com/embed/FxsJ8zEONTs</a></p><br />
<br />
</div><!--end news--><br />
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<img style="margin: 15px;" src="https://static.igem.org/mediawiki/2012hs/6/63/IGEMS-Team1roundcorners.jpg" width="210px"></img><br />
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<img style="margin: 15px; border-radius: 10px; border: 1px solid grey;" src="https://static.igem.org/mediawiki/2012hs/8/80/DSC04439.JPG" width="210px"></img><br />
<img style="margin: 10px;" src="https://static.igem.org/mediawiki/2012hs/d/dd/Online-store-link.png" width="210px"></img><br />
<img style="margin: 10px;" src="https://static.igem.org/mediawiki/2012hs/9/93/PinkS.png" width="210px"></img><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:22:20Z<p>Dniopek: </p>
<hr />
<div>''Italic text''{{TopII}}<br />
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun expose than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and integrated into trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to encourage curiosity. With the establishment of a virtual ‘Online Shop’ we tried to put genetically modified organisms in a modern and familiar background. As a open-minded part of the society, this should encourage the reader to think again about synthetic biology and to begin controversial discussions about this upcoming field of science. <br />
<br/><br />
For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few months, the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But furthermore we have found difficulties that should be optimised in the future.<br />
We have worked with enthusiasm on the bacteria tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterisation of different members of the same gene family as precA. So Our assumption is that different rec genes are activated based on a certain characteristic extension of DNA damage dependent on UV radiation. By linking these genes together we want to generate a more gradual UV-response for the detection of qualitative UV-intensity. At UV-intensities different genes are activated and a color gradient appears. This gradient will be used to determine the exact amount of radiation. This is important to give the bacteria tool kit the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and precC, they already gave us a positive result, so that our assumption was right. Now we have to analyse the data to find their most effective use. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analysed if the construct would also work with radioactive radiation. Radioactive radiation causes different DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMs collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further measurement systems to detect GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm. Therefore we can make GFP visible using blue LEDs. Furthermore we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. We could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
For the production of the jewelry, which shall be once a common product for the public, we need to simplify this system. Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue, when it is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way to recognize UV-radiation, which does not need any further background in synthetic biology. At the moment our bacteria turn blue after 10 min exposure. But the human body can resist much more UV-light without getting DNA-damage. We have to find a rec gene that show the most similarities with the human body. An other possibility to handle this problem would be to vary the UV permeability of the bacteria containment.<br />
<br/><br />
<br />
Some more problems that came out while working on the constructs. At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
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</html></div>Dniopekhttp://2012hs.igem.org/File:Outdoor-Test.pngFile:Outdoor-Test.png2012-06-17T02:19:31Z<p>Dniopek: uploaded a new version of &quot;File:Outdoor-Test.png&quot;</p>
<hr />
<div></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:16:42Z<p>Dniopek: </p>
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun expose than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application:<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS</a>. iGEMS are small tubes filled with bacterial suspension and trailed to trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to pique the reader’s curiosity and to present our project in an easy-to-understand way. With the establishment of a fictitious ‘Online Shop’ we tried to put genetically modified organisms in a modern and familiar background. As a open-minded part of the society, this should encourage the reader to think again about synthetic biology and to begin controversial discussions about this upcoming field of science. <br />
<br/><br />
For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few month the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But furthermore we have found difficulties that should be optimised in the future.<br />
We have worked with enthusiasm on the bacteria tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterisation of different members of the same gene family as precA. So Our assumption is that different rec genes are activated based on a certain characteristic extension of DNA damage dependent on UV radiation. By linking these genes together we want to generate a more gradual UV-response for the detection of qualitative UV-intensity. At UV-intensities different genes are activated and a color gradient appears. This gradient will be used to determine the exact amount of radiation. This is important to give the bacteria tool kit the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and precC, they already gave us a positive result, so that our assumption was right. Now we have to analyse the data to find their most effective use. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analysed if the construct would also work with radioactive radiation. Radioactive radiation causes different DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMs collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further measurement systems to detect GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm. Therefore we can make GFP visible using blue LEDs. Furthermore we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. We could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
For the production of the jewelry, which shall be once a common product for the public, we need to simplify this system. Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue, when it is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way to recognize UV-radiation, which does not need any further background in synthetic biology. At the moment our bacteria turn blue after 10 min exposure. But the human body can resist much more UV-light without getting DNA-damage. We have to find a rec gene that show the most similarities with the human body. An other possibility to handle this problem would be to vary the UV permeability of the bacteria containment.<br />
<br/><br />
<br />
Some more problems that came out while working on the constructs. At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
<br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:15:56Z<p>Dniopek: </p>
<hr />
<div>''Italic text''{{TopII}}<br />
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun expose than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application: iGEMS <a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS </a>. iGEMS are small tubes filled with bacterial suspension and trailed to trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to pique the reader’s curiosity and to present our project in an easy-to-understand way. With the establishment of a fictitious ‘Online Shop’ we tried to put genetically modified organisms in a modern and familiar background. As a open-minded part of the society, this should encourage the reader to think again about synthetic biology and to begin controversial discussions about this upcoming field of science. <br />
<br/><br />
For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few month the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But furthermore we have found difficulties that should be optimised in the future.<br />
We have worked with enthusiasm on the bacteria tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterisation of different members of the same gene family as precA. So Our assumption is that different rec genes are activated based on a certain characteristic extension of DNA damage dependent on UV radiation. By linking these genes together we want to generate a more gradual UV-response for the detection of qualitative UV-intensity. At UV-intensities different genes are activated and a color gradient appears. This gradient will be used to determine the exact amount of radiation. This is important to give the bacteria tool kit the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and precC, they already gave us a positive result, so that our assumption was right. Now we have to analyse the data to find their most effective use. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analysed if the construct would also work with radioactive radiation. Radioactive radiation causes different DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMs collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further measurement systems to detect GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm. Therefore we can make GFP visible using blue LEDs. Furthermore we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. We could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
For the production of the jewelry, which shall be once a common product for the public, we need to simplify this system. Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue, when it is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way to recognize UV-radiation, which does not need any further background in synthetic biology. At the moment our bacteria turn blue after 10 min exposure. But the human body can resist much more UV-light without getting DNA-damage. We have to find a rec gene that show the most similarities with the human body. An other possibility to handle this problem would be to vary the UV permeability of the bacteria containment.<br />
<br/><br />
<br />
Some more problems that came out while working on the constructs. At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
<br />
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<hr />
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun expose than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful constructions and tests we began to carefully characterize our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as cloning it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are able to embed this new and innovative UV-detection system in a fancy application: iGEMS<a href="http://2012HS.igem.org/Team:Heidelberg_LSL/Online_store" > iGEMS <a/>. iGEMS are small tubes filled with bacterial suspension and trailed to trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to pique the reader’s curiosity and to present our project in an easy-to-understand way. With the establishment of a fictitious ‘Online Shop’ we tried to put genetically modified organisms in a modern and familiar background. As a open-minded part of the society, this should encourage the reader to think again about synthetic biology and to begin controversial discussions about this upcoming field of science. <br />
<br/><br />
For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few month the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But furthermore we have found difficulties that should be optimised in the future.<br />
We have worked with enthusiasm on the bacteria tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterisation of different members of the same gene family as precA. So Our assumption is that different rec genes are activated based on a certain characteristic extension of DNA damage dependent on UV radiation. By linking these genes together we want to generate a more gradual UV-response for the detection of qualitative UV-intensity. At UV-intensities different genes are activated and a color gradient appears. This gradient will be used to determine the exact amount of radiation. This is important to give the bacteria tool kit the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and precC, they already gave us a positive result, so that our assumption was right. Now we have to analyse the data to find their most effective use. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analysed if the construct would also work with radioactive radiation. Radioactive radiation causes different DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMs collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further measurement systems to detect GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm. Therefore we can make GFP visible using blue LEDs. Furthermore we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. We could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
For the production of the jewelry, which shall be once a common product for the public, we need to simplify this system. Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue, when it is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way to recognize UV-radiation, which does not need any further background in synthetic biology. At the moment our bacteria turn blue after 10 min exposure. But the human body can resist much more UV-light without getting DNA-damage. We have to find a rec gene that show the most similarities with the human body. An other possibility to handle this problem would be to vary the UV permeability of the bacteria containment.<br />
<br/><br />
<br />
Some more problems that came out while working on the constructs. At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
<br />
<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
<br />
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<h2>Human Practice</h2><br />
<br />
</html><br />
<br />
As the Heidelberg Life-Science Lab is a place where science meets interested pupils, for us, it is very important to draw the connection between our scientific work and the non-scientific community. Many people are not aware of synthetic biology and only a minority is really informed about its potential. With our work, we want to encourage non-scientific readers to negotiate possible prejudices, learn more about our work in the lab and have an entertaining insight into the various applications of our new biological system. <br />
<br />
<html><br />
<br />
<h4>How can high-school students learn about genetic engineering?</h4><br />
<br />
<br />
<p>Learning by heart belongs to the past. We are learning by doing. Being normal high-school students, we want to show that everybody can understand the basic principles of how genetic circuits in cells can be 'engineered' and how intelligible most of the methods are. With our many explanations and protocols, we want to support, encourage and introduce upcoming high-school teams, interested teenagers, students and adults without vast knowledge to get involved into synthetic and molecular biology.</p><br />
<br />
<br />
<h4>Science interesting and intelligible - why not writing a book?</h4><br />
<br />
<p>This is the idea and motivation of the popular german scientific writer Dr. Olaf Fritsche. His upcoming book deals with the future of biological sciences. He will explain how scientific knowledge arises and show that everybody can understand facts when asking the right questions in science. Olaf approached us some weeks ago and asked whether he could visit our iGEM HS team in order to ask some basic questions about our project and team. Finally, he did not only visit us once, but several times, both inside and outside of the laboratory and followed our project from its start with the idea to its final end (his last visit was in the wiki-freeze night and he will follow up also after the iGEM jamboree). Its our great pleasure and honor, that Olaf Fritsche will report about our teams' and projects' development during taking part in the iGEM HS competition in his book in several passages embedded in different chapters. Fritsche’s work will show that establishing an iGEM project extracurricularly is ambitious but in the same way funny and intelligible. We hope that his book will encourage people outside of the synthetic biology community to overcome prejudices against science, in particular molecular biology and synthetic biology and become more educated and informed about modern life-sciences developments.</p><br />
<br />
<br />
<h4>Why iGEMs and an Online Shop?</h4><br />
<br />
<p>We carefully thought about possible applications of our UV-measuring system and decided that we should work in direction of an everyday-life product of which the general public should have most profits.<br />
For the integration of our measurment construct containing bacteria into such a product context, we thought about a funny and innovative way that could be coherent with current trends and that should evoke interest and curiosity amongst people. iGEMs, genetically modified gems, on bracelets and necklaces are a nice way to communicate our ideas to a great public.<br/><br />
<br />
The way we communicate our products is an online store <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Online_store">‘Online store’</a> which is - of course - not a real store, but just a fake (you can neither buy the iGEMS, nor the UV-sensing bacteria or anything). supposed to give the user a reality-like impression of an everyday-life product which could be developed by using synthetic biology approaches. However, we tried to give it an authentic outfit in order to show that a similar version would be possible in future. To overcome the distance to our product, we tried to imitate with our ‘Online Shop’ common structure and design. As the customer nowadays is able to go online shopping, we arranged a similar option with our ‘Check out’ bottom in our ‘Online Store’. Here, interested readers can pre book an article and should explain briefly why the product would be most suitable especially for them. We would like to use this information to get an impression of the wishes of interested people in order to optimize our products and to get better insight of the common favour. Doing so, we want to show that we are eager to communicate with the non-scientific public and to encourage them in particular to think about considering iGEMs as a general possible application of small genetically engineered machines. </p><br />
<br />
Our idea of monitoring the intensity of the UV-radiation mediated by the sun goes along with the efforts of the Heidelberg German Cancer Reasearch Center for cancer prevention. This is due to the fact that continuous high doses of UV-radiation are one major risc factor for melanoma development. One of our major goals is to achieve more general awareness of cancer risks and cancer prevention possibilities. In our case we want to make sunbathing teenagers more aware of the enhanced risk to develop malign melanoma (black skin cancer) by getting sunburned and being exposed to intense UV-radiation frequently.<br/><br />
<br />
<h4>Watch us on biotechnologie.tv! (in German language)</h4><br />
<br />
<p>We are very happy that the online information platform ‘biotechnologie.de’ involved an interview with one of our participants, Charlotte Bunne, in one of their short documentations. Initiated by the German ministry for education and science, <br />
<a href="http://www.biotechnologie.de/">biotechnologie.de</a><br />
is a public platform to inform not only scientists, but also people without scientific backround about current results and technologies in biotechnological research. One of its main rubrics is a large video collection with short videos about many various areas associated with biotechnology. The platform always is up to date and we are very glad to be in the latest episode of biotechnologie.tv. Here, Charlotte explains briefly and very intelligible our idea, our concept and what we are doing right now. You can watch the interview at the bottom of the page. It starts at 3:55 min.</p></br><br />
<br />
<iframe width="600px" height="400px" style="margin-left:20px;" src="http://www.youtube.com/embed/FxsJ8zEONTs" frameborder="0" allowfullscreen ><p>If the video doesn't appear here, please try reloading this page once or use the link below!</p></iframe></br></br><br />
<p> If the video doesn't appear here, please try reloading this page once or use the following link!</p><br />
<p>Link to the video: <a href="http://www.youtube.com/embed/FxsJ8zEONTs">http://www.youtube.com/embed/FxsJ8zEONTs</a></p><br />
<br />
</div><!--end news--><br />
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<img style="margin: 15px;" src="https://static.igem.org/mediawiki/2012hs/6/63/IGEMS-Team1roundcorners.jpg" width="210px"></img><br />
<img style="margin: 5px; border-radius: 10px; border: 1px solid grey;" src="https://static.igem.org/mediawiki/2012hs/8/88/LifeScience_Lab_0027-klein.jpg" width="210px"></img><br />
<img style="margin: 15px; border-radius: 10px; border: 1px solid grey;" src="https://static.igem.org/mediawiki/2012hs/8/80/DSC04439.JPG" width="210px"></img><br />
<img style="margin: 10px;" src="https://static.igem.org/mediawiki/2012hs/d/dd/Online-store-link.png" width="210px"></img><br />
<img style="margin: 10px;" src="https://static.igem.org/mediawiki/2012hs/9/93/PinkS.png" width="210px"></img><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:09:30Z<p>Dniopek: </p>
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<h2> Summary & Outlook</h2><br />
<br />
<br />
<br />
<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012hs/0/09/Gruppe3.png" width="670" style="margin-left:15px;"></img></br><br />
</center><br />
<br />
<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
<br/><br />
All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun expose than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real-life tests emphasize the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
<br/><br />
<br/><br />
After these successful construtions and tests we began to characterize carefully our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as clone it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
<br/><br />
<br/><br />
With this unpredictable success, we are glad to be able to bed this new and innovative UV-detecting system in a fancy application: iGEMS. iGEMS are small tubes filled with bacterial suspension and trailed to trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to pique the reader’s curiosity and to present our project in an easy-to-understand way. With the establishment of a fictitious ‘Online Shop’ we tried to put genetically modified organisms in a modern and familiar background. As a open-minded part of the society, this should encourage the reader to think again about synthetic biology and to begin controversial discussions about this upcoming field of science. <br />
<br/><br />
For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
<br />
<br />
<h4>Outlook</h4><br />
<p><br />
In the past few month the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But furthermore we have found difficulties that should be optimised in the future.<br />
We have worked with enthusiasm on the bacteria tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
<br/><br />
<br/><br />
In the future we want to create a clearer characterisation of different members of the same gene family as precA. So Our assumption is that different rec genes are activated based on a certain characteristic extension of DNA damage dependent on UV radiation. By linking these genes together we want to generate a more gradual UV-response for the detection of qualitative UV-intensity. At UV-intensities different genes are activated and a color gradient appears. This gradient will be used to determine the exact amount of radiation. This is important to give the bacteria tool kit the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and precC, they already gave us a positive result, so that our assumption was right. Now we have to analyse the data to find their most effective use. <br />
<br/><br />
<br/><br />
For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analysed if the construct would also work with radioactive radiation. Radioactive radiation causes different DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.<br />
<br/><br />
<br/><br />
We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMs collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further measurement systems to detect GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm. Therefore we can make GFP visible using blue LEDs. Furthermore we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. We could generate a color range from green to orange to red with just one single blue LED.<br />
<br/><br />
<br/><br />
For the production of the jewelry, which shall be once a common product for the public, we need to simplify this system. Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue, when it is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way to recognize UV-radiation, which does not need any further background in synthetic biology. At the moment our bacteria turn blue after 10 min exposure. But the human body can resist much more UV-light without getting DNA-damage. We have to find a rec gene that show the most similarities with the human body. An other possibility to handle this problem would be to vary the UV permeability of the bacteria containment.<br />
<br/><br />
<br />
Some more problems that came out while working on the constructs. At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
<br/><br />
Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
<br/><br />
In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
<br/><br />
<br/><br />
We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
<br/><br />
</p><br />
<br />
</div><br />
<br />
<div id="news"><br />
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<h4>Achievements</h4><br />
<table id="achievements" cellpadding="7" cellspacing="8"><br />
<tr><br />
<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
<tr><br />
<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
</tr><br />
</table><br />
<p></p><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_humanPracticeTeam:Heidelberg LSL/Project humanPractice2012-06-17T02:08:45Z<p>Dniopek: </p>
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<h2>Human Practice</h2><br />
<br />
</html><br />
<br />
As the Heidelberg Life-Science Lab is a place where science meets interested pupils, for us, it is very important to draw the connection between our scientific work and the non-scientific community. Many people are not aware of synthetic biology and only a minority is really informed about its potential. With our work, we want to encourage non-scientific readers to negotiate possible prejudices, learn more about our work in the lab and have an entertaining insight into the various applications of our new biological system. <br />
<br />
<html><br />
<br />
<h4>How can high-school students learn about genetic engineering?</h4><br />
<br />
<br />
<p>Learning by heart belongs to the past. We are learning by doing. Being normal high-school students, we want to show that everybody can understand the basic principles of how genetic circuits in cells can be 'engineered' and how intelligible most of the methods are. With our many explanations and protocols, we want to support, encourage and introduce upcoming high-school teams, interested teenagers, students and adults without vast knowledge to get involved into synthetic and molecular biology.</p><br />
<br />
<br />
<h4>Science interesting and intelligible - why not writing a book?</h4><br />
<br />
<p>This is the idea and motivation of the popular german scientific writer Dr. Olaf Fritsche. His upcoming book deals with the future of biological sciences. He will explain how scientific knowledge arises and show that everybody can understand facts when asking the right questions in science. Olaf approached us some weeks ago and asked whether he could visit our iGEM HS team in order to ask some basic questions about our project and team. Finally, he did not only visit us once, but several times, both inside and outside of the laboratory and followed our project from its start with the idea to its final end (his last visit was in the wiki-freeze night and he will follow up also after the iGEM jamboree). Its our great pleasure and honor, that Olaf Fritsche will report about our teams' and projects' development during taking part in the iGEM HS competition in his book in several passages embedded in different chapters. Fritsche’s work will show that establishing an iGEM project extracurricularly is ambitious but in the same way funny and intelligible. We hope that his book will encourage people outside of the synthetic biology community to overcome prejudices against science, in particular molecular biology and synthetic biology and become more educated and informed about modern life-sciences developments.</p><br />
<br />
<br />
<h4>Why iGEMs and an Online Shop?</h4><br />
<br />
<p>We carefully thought about possible applications of our UV-measuring system and decided that we should work in direction of an everyday-life product of which the general public should have most profits.<br />
For the integration of our measurment construct containing bacteria into such a product context, we thought about a funny and innovative way that could be coherent with current trends and that should evoke interest and curiosity amongst people. iGEMs, genetically modified gems, on bracelets and necklaces are a nice way to communicate our ideas to a great public.<br/><br />
<br />
Our idea of monitoring the intensity of the UV-radiation mediated by the sun goes along with the efforts of the Heidelberg German Cancer Reasearch Center for cancer prevention. This is due to the fact that continuous high doses of UV-radiation are one major risc factor for melanoma development. One of our major goals is to achieve more general awareness of cancer risks and cancer prevention possibilities. In our case we want to make sunbathing teenagers more aware of the enhanced risk to develop malign melanoma (black skin cancer) by getting sunburned and being exposed to intense UV-radiation frequently.<br/><br />
<br />
Presenting online store <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Online_store">‘Online store’</a> is - of course - just a fake (you can neither buy the iGEMS, nor the UV-sensing bacteria or anything). But it is supposed to give the user a reality-like impression of an everyday-life product which could be developed by using synthetic biology approaches. However, we tried to give it an authentic outfit in order to show that a similar version would be possible in future. To overcome the distance to our product, we tried to imitate with our ‘Online Shop’ common structure and design. As the customer nowadays is able to go online shopping, we arranged a similar option with our ‘Check out’ bottom in our ‘Online Store’. Here, interested readers can pre book an article and should explain briefly why the product would be most suitable especially for them. We would like to use this information to get an impression of the wishes of interested people in order to optimize our products and to get better insight of the common favour. Doing so, we want to show that we are eager to communicate with the non-scientific public and to encourage them in particular to think about considering iGEMs as a general possible application of small genetically engineered machines. </p><br />
<br />
<h4>Watch us on biotechnologie.tv! (in German language)</h4><br />
<br />
<p>We are very happy that the online information platform ‘biotechnologie.de’ involved an interview with one of our participants, Charlotte Bunne, in one of their short documentations. Initiated by the German ministry for education and science, <br />
<a href="http://www.biotechnologie.de/">biotechnologie.de</a><br />
is a public platform to inform not only scientists, but also people without scientific backround about current results and technologies in biotechnological research. One of its main rubrics is a large video collection with short videos about many various areas associated with biotechnology. The platform always is up to date and we are very glad to be in the latest episode of biotechnologie.tv. Here, Charlotte explains briefly and very intelligible our idea, our concept and what we are doing right now. You can watch the interview at the bottom of the page. It starts at 3:55 min.</p></br><br />
<br />
<iframe width="600px" height="400px" style="margin-left:20px;" src="http://www.youtube.com/embed/FxsJ8zEONTs" frameborder="0" allowfullscreen ><p>If the video doesn't appear here, please try reloading this page once or use the link below!</p></iframe></br></br><br />
<p> If the video doesn't appear here, please try reloading this page once or use the following link!</p><br />
<p>Link to the video: <a href="http://www.youtube.com/embed/FxsJ8zEONTs">http://www.youtube.com/embed/FxsJ8zEONTs</a></p><br />
<br />
</div><!--end news--><br />
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<img style="margin: 15px;" src="https://static.igem.org/mediawiki/2012hs/6/63/IGEMS-Team1roundcorners.jpg" width="210px"></img><br />
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<img style="margin: 15px; border-radius: 10px; border: 1px solid grey;" src="https://static.igem.org/mediawiki/2012hs/8/80/DSC04439.JPG" width="210px"></img><br />
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</html></div>Dniopekhttp://2012hs.igem.org/Team:Heidelberg_LSL/Project_SummaryTeam:Heidelberg LSL/Project Summary2012-06-17T02:08:33Z<p>Dniopek: </p>
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<h2> Summary & Outlook</h2><br />
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<h4>Lying in the sun we now have time to recover and think about the past weeks…</h4><br />
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<h4>Summary</h4><br />
<p> After a long phase of planning and development, we sucessfully developed a new biological system in <i>E.coli</i> that reacts quantitatively to UV-radiation in both artificial, and natural doses of radiation. <br/><br />
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All of our promoters, precA, <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and psulA, which are linked with the SOS-response, reacted reliably to UV-induced damages and started expression of our reporter genes GFP and LacZ. All combinations such as <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, precA_GFP, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> and psulA_GFP worked regarding quantitative UV-detection, which is an unexpected success. Outdoor tests with our lacZ-reporter constructs in sunlight and in shade indicated a light-dependent color change which is visible even to the naked eye. The color of the exposed bacterial suspension changed in the course of irradiation time from light-yellow to a dark greenish blue. This color change is a result of the UV-induced expression of the enzyme beta-galactosidase which catalyzes the reaction of X-Gal to a blue product. With our test series, we furthermore discovered significant differences in the strength of UV-detection between rec- and sulA-promotors. <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a> constructs for instance showed less visible color change after 8 minutes of sun expose than <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a> constructs.<br/><br />
These successful real life tests give evidence to the possibility to use our biological systems in a real applicational context such as our iGEM-jewelry collection. <br />
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After these successful construtions and tests we began to characterize carefully our new biological systems and submitted four new entries to the parts registry. To make this important contribution to the synthetic biology community and to the partsregistry, we adjusted our constructs to the official requirements of the partsregistry such as clone it into the pSB1C3 plasmid backbone with the required pre- and suffix. Our new three parts are <a href="http://partsregistry.org/Part:BBa_K862000"> precA_LacZ</a>, <a href="http://partsregistry.org/Part:BBa_K862002">precB_LacZ</a> and <a href="http://partsregistry.org/Part:BBa_K862000">psulA_LacZ</a>. Additionally, we looked up, ordered, tested and registered the sequence of the recB- promotor, which was not yet available in the registry. First tests showed that UV-induced activity of <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> can be compared with those of precA.<br />
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With this unpredictable success, we are glad to be able to bed this new and innovative UV-detecting system in a fancy application: iGEMS. iGEMS are small tubes filled with bacterial suspension and trailed to trendy necklaces and bracelets. We developed this fictitious product in order to raise the public awareness of the “invisible danger”, to pique the reader’s curiosity and to present our project in an easy-to-understand way. With the establishment of a fictitious ‘Online Shop’ we tried to put genetically modified organisms in a modern and familiar background. As a open-minded part of the society, this should encourage the reader to think again about synthetic biology and to begin controversial discussions about this upcoming field of science. <br />
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For us personally, the project broadened our scientific horizon and gave us the opportunity to learn how to work scientifically. We had a great time together and look forward to present our work to you at the Jamboree 2012.<br />
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<h4>Outlook</h4><br />
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In the past few month the project was brought up to a great level. We have constructed different BioBricks which were sent to the partsregistry, tested our parts and designed a jewelry collection. But furthermore we have found difficulties that should be optimised in the future.<br />
We have worked with enthusiasm on the bacteria tool kit which can detect UV-radiation and have made a lot of progress, therefore we want to continue working on it.<br />
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In the future we want to create a clearer characterisation of different members of the same gene family as precA. So Our assumption is that different rec genes are activated based on a certain characteristic extension of DNA damage dependent on UV radiation. By linking these genes together we want to generate a more gradual UV-response for the detection of qualitative UV-intensity. At UV-intensities different genes are activated and a color gradient appears. This gradient will be used to determine the exact amount of radiation. This is important to give the bacteria tool kit the properties of a dosimeter. We started testing <a href="http://partsregistry.org/Part:BBa_K862003">precB</a> and precC, they already gave us a positive result, so that our assumption was right. Now we have to analyse the data to find their most effective use. <br />
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For the iGEM competition we only tested our constructs by exposing the samples to UV light. We have not analysed if the construct would also work with radioactive radiation. Radioactive radiation causes different DNA damage, therefore it is not sure if precA becomes activated in the same way as with UV radiation. In principle, we assume that the induction by radioactive radiation would work as the precA promoter is known to be activated by the induced damage. We could not test our constructs with radioactive radiation, because we did not have the right equipment. But we have got a contact person who could give us access to the required machines.<br />
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We also want to include the constructs with a GFP reporter instead of LacZ in our iGEMs collection. GFP cannot be seen by a color change of the bacterial suspension and normally you need further measurement systems to detect GFP, because it has to be excited by a specific wavelength. To overcome this challenge, we want to combine the iGEMS with LED lamps. LEDs emit light of a certain wavelength, e.g. blue light with 460 nm. Therefore we can make GFP visible using blue LEDs. Furthermore we could also develop a construct with dtTomato, a very light, red-orange fluorescent protein, as another reporter. We could generate a color range from green to orange to red with just one single blue LED.<br />
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For the production of the jewelry, which shall be once a common product for the public, we need to simplify this system. Only the rec gene is needed that gives us the best adaptation to the human body. The bacterial suspension shall turn blue, when it is irradiated by too much sunlight, that is dangerous for the human. That is a really easy way to recognize UV-radiation, which does not need any further background in synthetic biology. At the moment our bacteria turn blue after 10 min exposure. But the human body can resist much more UV-light without getting DNA-damage. We have to find a rec gene that show the most similarities with the human body. An other possibility to handle this problem would be to vary the UV permeability of the bacteria containment.<br />
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Some more problems that came out while working on the constructs. At the moment we have to add X-Gal after the exposure to UV, because UV-radiation may degrade X-Gal. But when producing locked vial, X-Gal cannot added additional. Therefore we have to find by researching different ways to deal with X-Gal.<br />
Furthermore we have to take account of the use of suncream in connection with our iGEMs, because suncream delays the effect of UV-light. <br />
We are optimistic that we can find solutions to all problems and develop our project to a industrial standard. <br />
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Our project comprises a novel idea and opens up new perspectives, especially in Human Practices. The integration of the bacteria into jewelry represents a completely new and consumer-friendly solution to give the public an understanding of synthetic biology. <br />
We found an easy way for everyone to protect themselves from sunburn. The method to use dosimeters is old-fashioned, complicated and expensive. Lying on the beach, nobody uses dosimeters because they are too big and uncomfortable. Not only do our fancy iGEMS warn people of too intense UV-radiation but also are trendy accessories! People who wear our iGEMS are aware of the invisible danger and encourage other people to follow this upcoming trend. <br />
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In cooperation with the biotechnological companies and the jewelry industry we could develop a new branch of sustainable applications for biological systems.<br />
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We are looking forward to the iGem HS Jamboree 2012 and present our work to the other teams from all over the world. We hope to get in contact with other biology interested pupils and present them our iGEMS.<br />
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<h4>Achievements</h4><br />
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<td align="justify" width="230px"><b>Three</b> different <b>UV-Sensor parts constructed</b> and <b>submitted</b> to the <b>registry</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
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<td align="justify" ><b>All parts</b> were <b>characterized carefully</b> and show a <b>UV-dose-dependent color-production</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
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<td align="justify" ><b>Outdoor-Test</b> shows our parts to be <b>working in a real application</b> context</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
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<td align="justify" ><b>Human Practices</b> - consumer product development for raising the <b>public awareness</b> of the <b>“invisible danger”</b> and <b>presenting our project</b> in an <b>easy-to-understand</b> way</td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
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<td align="justify" >Finally, we <b>learned a lot</b> about synthetic biology, laboratory methods and had a <b>great time</b> working together on our project, and are now <b>looking forward</b> to meeting the other teams at the <b>iGEM HS Jamboree!</b></td><br />
<td><img src="https://static.igem.org/mediawiki/2012hs/7/72/Checkbox.png" width="30px"></img></td><br />
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