Team:Heidelberg LSL/Notebook materials

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iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Kits for Plasmid and DNA purification

Name	Product Description	Company
PureYield™ Plasmid Miniprep System	small scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains	Promega
QIAprep Spin MiniPrep Kit (50)	small scale plasmid purification from E. coli Top10 cells before sequencing	Qiagen
PureYield™ Plasmid Midiprep System	medium scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains	Promega
QIAquick Nucleotide Removal Kit	direct purification of DNA after restriction digests	Qiagen
QIAquick Gel Extraction Kit (50)	Purification of DNA fragements from agarose gel	Qiagen

Enzymes

Name	Product Description	Company
EcoRI	restriction enzyme, recognition sequence: GAATTC	Promega or NEB
SpeI	restriction enzyme, recognition sequence: ACTAGT	Promega or NEB
PureYield™ Plasmid Midiprep System	restriction enzyme, recognition sequence: TCTAGA	Promega or NEB
PstI	restriction enyzme, recognition sequence: CTGCAG	Promega or NEB
T4 DNA ligase	Ligation of DNA fragments	Promega or Fermentas
2x PCR MasterMix	Standard Taq PCR master mix for robust amplification of DNA-Fragments by PCR; used for colony-PCR screens	Fermentas

Oligonucleotides

Oligos were annealed and subsequently cloned into EcoRI/XbaI predigested reporter backbones. Thereby short synthetic DNA-sequences (in this case for the promoters psulA, precA and precB) can be generated in a fast and easy manner.

Oligo Name	Oligo Sequence	sequence length

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