"
Team:Heidelberg LSL/Notebook materials
From 2012hs.igem.org
(Difference between revisions)
| Line 142: | Line 142: | ||
<tr> | <tr> | ||
<p> | <p> | ||
| - | <td>psulA_fw</td><td> | + | <td>psulA_fw</td><td>5'-aattcgcggccgcttctagagGGGTTGATCTTTGTTGT CACTGGATGTACTGTACATCCATACAGTAACTCACc-3'</td><td>74</td> |
</p> | </p> | ||
</tr> | </tr> | ||
| Line 148: | Line 148: | ||
<tr> | <tr> | ||
<p> | <p> | ||
| - | <td>psulA_rev</td><td> | + | <td>psulA_rev</td><td>5'-ctaggGTGAGTTACTGTATGGATGTACAGTACATCCAG TGACAACAAAGATCAACCCctctagaagcggccgcg-3'</td><td>74</td> |
</p> | </p> | ||
</tr> | </tr> | ||
| Line 154: | Line 154: | ||
<tr> | <tr> | ||
<p> | <p> | ||
| - | <td>precB_fw</td><td> | + | <td>precB_fw</td><td>5'aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGA ACGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3'</td><td>92</td> |
</p> | </p> | ||
</tr> | </tr> | ||
| Line 160: | Line 160: | ||
<tr> | <tr> | ||
<p> | <p> | ||
| - | <td>precB_rev</td><td> | + | <td>precB_rev</td><td>5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTG ACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'</td><td>92</td> |
</p> | </p> | ||
</tr> | </tr> | ||
| Line 166: | Line 166: | ||
<tr> | <tr> | ||
<p> | <p> | ||
| - | <td>precC_fw</td><td> | + | <td>precC_fw</td><td>5'-aattcgcggccgcttctagagTTCACCCGGGGGCAGAGAAGGC GAGATGACCCGCCTGCATTGCCCGAATCGTCAGTAGTCAGGAGCCGCTc-3'</td><td>92</td> |
</p> | </p> | ||
</tr> | </tr> | ||
| Line 172: | Line 172: | ||
<tr> | <tr> | ||
<p> | <p> | ||
| - | <td>precC_rev</td><td> | + | <td>precC_rev</td><td>5'-ctaggAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCG GGTCATCTCGCCTTCTCTGCCCCCGGGTGAActctagaagcggccgcg-3'</td><td>92</td> |
</p> | </p> | ||
</tr> | </tr> | ||
Revision as of 14:32, 11 June 2012
Kits for Plasmid and DNA purification
| Name | Product Description | Company |
| small scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains | Promega | |
| small scale plasmid purification from E. coli Top10 cells before sequencing | Qiagen | |
| medium scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains | Promega | |
| direct purification of DNA after restriction digests | Qiagen | |
| Purification of DNA fragements from agarose gel | Qiagen |
Enzymes
| Name | Product Description | Company |
| restriction enzyme, recognition sequence: GAATTC | Promega or NEB | |
| restriction enzyme, recognition sequence: ACTAGT | Promega or NEB | |
| restriction enzyme, recognition sequence: TCTAGA | Promega or NEB | |
| restriction enyzme, recognition sequence: CTGCAG | Promega or NEB | |
| Ligation of DNA fragments | Promega or Fermentas | |
| Standard Taq PCR master mix for robust amplification of DNA-Fragments by PCR; used for colony-PCR screens | Fermentas |
Oligonucleotides
Oligos were annealed and subsequently cloned into EcoRI/XbaI predigested reporter backbones. Thereby short synthetic DNA-sequences (in this case for the promoters psulA, precA and precB) can be generated in a fast and easy manner.
| Oligo Name | Oligo Sequence | sequence length |
| psulA_fw | 5'-aattcgcggccgcttctagagGGGTTGATCTTTGTTGT CACTGGATGTACTGTACATCCATACAGTAACTCACc-3' | 74 |
| psulA_rev | 5'-ctaggGTGAGTTACTGTATGGATGTACAGTACATCCAG TGACAACAAAGATCAACCCctctagaagcggccgcg-3' | 74 |
| precB_fw | 5'aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGA ACGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' | 92 |
| precB_rev | 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTG ACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3' | 92 |
| precC_fw | 5'-aattcgcggccgcttctagagTTCACCCGGGGGCAGAGAAGGC GAGATGACCCGCCTGCATTGCCCGAATCGTCAGTAGTCAGGAGCCGCTc-3' | 92 |
| precC_rev | 5'-ctaggAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCG GGTCATCTCGCCTTCTCTGCCCCCGGGTGAActctagaagcggccgcg-3' | 92 |
