LOCUS       NEWMOL                  5375 bp    DNA     circular      6-JUN-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|628167182|
COMMENT     VNTDBDATE|628167182|
COMMENT     LSOWNER|
COMMENT     VNTNAME|NEWMOL|
COMMENT     VNTAUTHORNAME|BioQuant|
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FEATURES             Location/Qualifiers
     promoter        22..92
                     /vntifkey="30"
                     /label=RecB
     CDS             complement(3597..4194)
                     /vntifkey="4"
                     /label=Origin\pMB1
     CDS             complement(4487..5263)
                     /vntifkey="4"
                     /label=Chloramphenicol\Resistance
     terminator      complement(4369..4474)
                     /vntifkey="43"
                     /label=T0\Terminator
     misc_feature    complement(3482..3501)
                     /vntifkey="21"
                     /label=VR\Sequencing\PBS
     primer_bind     5258..5277
                     /vntifkey="28"
                     /label=VF2\Sequencing\PBS
     CDS             117..3191
                     /vntifkey="4"
                     /label=LacZ
     terminator      3200..3279
                     /vntifkey="43"
                     /label=B0010
     terminator      3288..3328
                     /vntifkey="43"
                     /label=B0012
BASE COUNT     1312 a      1395 c      1428 g      1240 t 
ORIGIN
        1 gaattcgcgg ccgcttctag agcctgaagg ctggaaagtg tgggagaacg tcagcgcgtt 
       61 gcagcaaaca atgcccctga tgagtgaaaa gactagagaa agaggagaaa tactagatga 
      121 ccatgattac ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 
      181 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 
      241 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg 
      301 cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg 
      361 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca 
      421 ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg 
      481 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa 
      541 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt 
      601 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag 
      661 aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg 
      721 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac 
      781 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg 
      841 aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat 
      901 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg 
      961 agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt 
     1021 ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca 
     1081 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc 
     1141 tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc 
     1201 ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc 
     1261 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc 
     1321 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg 
     1381 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa 
     1441 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg 
     1501 aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt 
     1561 cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc 
     1621 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca 
     1681 aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg 
     1741 cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc 
     1801 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa 
     1861 acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt 
     1921 tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa 
     1981 aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg 
     2041 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta 
     2101 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg 
     2161 aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag 
     2221 tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc 
     2281 gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga 
     2341 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc 
     2401 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc 
     2461 gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca 
     2521 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag 
     2581 cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg 
     2641 cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta 
     2701 gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc 
     2761 ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag 
     2821 ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc 
     2881 cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga 
     2941 cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc 
     3001 gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag 
     3061 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc 
     3121 cgtcagtatc ggcggaattt cagctgagcg ccggtcgcta ccattaccag ttggtctggt 
     3181 gtcaaaaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 
     3241 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 
     3301 ccttcgggtg ggcctttctg cgtttataac tagtagcggc cgctgcagtc cggcaaaaaa 
     3361 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat 
     3421 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     3481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     3541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     3601 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     3661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     3721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     3781 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     3841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     3901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     3961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     4021 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 
     4081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     4141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     4201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     4261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     4321 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc 
     4381 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt 
     4441 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct 
     4501 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 
     4561 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat 
     4621 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa 
     4681 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt 
     4741 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa 
     4801 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat 
     4861 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg 
     4921 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat 
     4981 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg 
     5041 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc 
     5101 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag 
     5161 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt 
     5221 gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc 
     5281 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 
     5341 aaaaaatcct tagctttcgc taaggatgat ttctg 
//
