LOCUS       NEWMOL                  5496 bp    DNA     circular      6-JUN-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|628167182|
COMMENT     VNTDBDATE|628167182|
COMMENT     LSOWNER|
COMMENT     VNTNAME|NEWMOL|
COMMENT     VNTAUTHORNAME|BioQuant|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        22..212
                     /vntifkey="30"
                     /label=RecA
     CDS             238..3312
                     /vntifkey="4"
                     /label=LacZ
     terminator      3321..3400
                     /vntifkey="43"
                     /label=B0010
     terminator      3409..3449
                     /vntifkey="43"
                     /label=B0012
     RBS             220..231
                     /vntifkey="32"
                     /label=B0034
     CDS             complement(4608..5384)
                     /vntifkey="4"
                     /label=Chloramphenicol\Resistance
     terminator      complement(4490..4595)
                     /vntifkey="43"
                     /label=T0\Terminator
     misc_feature    complement(3718..4315)
                     /vntifkey="21"
                     /label=Origin\pMB1
     primer_bind     5379..5398
                     /vntifkey="28"
                     /label=VF2\Sequencing\Primer
     primer_bind     3603..3622
                     /vntifkey="28"
                     /label=VR\sequencing\primer
BASE COUNT     1362 a      1418 c      1443 g      1273 t 
ORIGIN
        1 gaattcgcgg ccgcttctag aacaatttct acaaaacact tgatactgta tgagcataca 
       61 gtataattgc ttcaacagaa catattgact atccggtatt acccggcatg acaggagtaa 
      121 aaatggctat cgacgaaaac aaacagaaag cgttggcggc agcactgggc cagattgaga 
      181 aacaatttgg taaaggctcc atcatgtaat aaactagaga aagaggagaa atactagatg 
      241 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
      301 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 
      361 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt 
      421 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag 
      481 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac 
      541 accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg 
      601 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga 
      661 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt 
      721 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga 
      781 gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag 
      841 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca 
      901 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg 
      961 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta 
     1021 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat 
     1081 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg 
     1141 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc 
     1201 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt 
     1261 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat 
     1321 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag 
     1381 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg 
     1441 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg 
     1501 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta 
     1561 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat 
     1621 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct 
     1681 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc 
     1741 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc 
     1801 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac 
     1861 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc 
     1921 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa 
     1981 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag 
     2041 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca 
     2101 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc 
     2161 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt 
     2221 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt 
     2281 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta 
     2341 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg 
     2401 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg 
     2461 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc 
     2521 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg 
     2581 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc 
     2641 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca 
     2701 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac 
     2761 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt 
     2821 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg 
     2881 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta 
     2941 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg 
     3001 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg 
     3061 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc 
     3121 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa 
     3181 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc 
     3241 ccgtcagtat cggcggaatt tcagctgagc gccggtcgct accattacca gttggtctgg 
     3301 tgtcaaaaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 
     3361 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 
     3421 accttcgggt gggcctttct gcgtttataa ctagtagcgg ccgctgcagt ccggcaaaaa 
     3481 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta 
     3541 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     3601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     3661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     3721 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     3781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     3841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     3901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     3961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     4021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     4081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     4141 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 
     4201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     4261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     4321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     4381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     4441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac 
     4501 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg 
     4561 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc 
     4621 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca 
     4681 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa 
     4741 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca 
     4801 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct 
     4861 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga 
     4921 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca 
     4981 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt 
     5041 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga 
     5101 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg 
     5161 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc 
     5221 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta 
     5281 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg 
     5341 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac 
     5401 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata 
     5461 aaaaaaatcc ttagctttcg ctaaggatga tttctg 
//
