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Team:SouthBendMishawakaIN
From 2012hs.igem.org
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===Project=== | ===Project=== | ||
| - | What are you working on this semester? | + | What are you working on this semester? |
| - | + | Arsenic is a naturally occurring toxic metal, and millions of people are exposed to this metalloid through contaminated drinking water worldwide. We propose to build an arsenic biosensor using the bacterial arsenic-sensitive promoter element, BBaK190015. In our previous experience, we have found that BBaK190015 has very high background activity and may be sensitive to tr...ace levels of arsenic and perhaps other metals such as copper. We unsuccessfully attempted to lower the sensitivity of BBaK190015 by isolating UV-resistant mutants and considered extending the 3’- end of the promoter to bind more ArsR inhibitors. However, we now propose to include the chemiluminescence as a reporter system. This will allow us to eliminate the background noise through the development of a battery powered iGlowi-I luminometer (Cambrigde 2010). We will design our device to have an internal standard for arsenic that should allow us to measure arsenic in the field. | |
===Notebook=== | ===Notebook=== | ||
Revision as of 18:59, 4 June 2012
- a team description
- project description
- safety information (did your team take a safety training course? were you supervised in the lab?)
- team attribution (who did what part of your project?)
- lab notebook
- sponsor information
- other information
Example: 2012hs.igem.org/Team:SouthBendMishawakaIN/Our_Pets
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. | |
|
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:SouthBendMishawakaIN team.png Your team picture |
| Team SouthBendMishawakaIN |
| Official Team Profile |
|---|
Contents |
Team
Tell us about your team, your school!
Project
What are you working on this semester? Arsenic is a naturally occurring toxic metal, and millions of people are exposed to this metalloid through contaminated drinking water worldwide. We propose to build an arsenic biosensor using the bacterial arsenic-sensitive promoter element, BBaK190015. In our previous experience, we have found that BBaK190015 has very high background activity and may be sensitive to tr...ace levels of arsenic and perhaps other metals such as copper. We unsuccessfully attempted to lower the sensitivity of BBaK190015 by isolating UV-resistant mutants and considered extending the 3’- end of the promoter to bind more ArsR inhibitors. However, we now propose to include the chemiluminescence as a reporter system. This will allow us to eliminate the background noise through the development of a battery powered iGlowi-I luminometer (Cambrigde 2010). We will design our device to have an internal standard for arsenic that should allow us to measure arsenic in the field.
Notebook
Show us how you spent your days.
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
What impact does/will your project have on the public?
Fun!
What was your favorite team snack?? Have a picture of your team mascot?
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