Team:SouthBendMishawakaIN

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<div id="template" style="text-align: center; font-weight: bold; font-size: large; /*color: #f6f6f6*/; padding: 5px;">
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; /*color: #f6f6f6*/; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have the following information on your wiki:
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<ul style="font-weight:normal;">
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<li>a team description</li>
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<li>project description</li>
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<li>safety information (did your team take a safety training course? were you supervised in the lab?)</li>
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<li>team attribution (who did what part of your project?)</li>
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<li>lab notebook</li>
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<li>sponsor information</li>
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REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2012hs.igem.org/Team:SouthBendMishawakaIN/Our_Pets</span>
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<td><center><h2>
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Welcome to the Homepage of the
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<br>
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DNA Architects
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<br>
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SouthBend MishawakaIN
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:SouthBendMishawakaIN_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:SouthBendMishawakaIN_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:SouthBendMishawakaIN | Team SouthBendMishawakaIN]]
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te_month=06&date_day=30&date_year=0&un=IGEM JAMBOREE 2012&size=normal&mo=06&da=30&yr=2012"
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!align="center"|[https://igem.org/Team.cgi?year=2012&division=high_school&team_name=SouthBendMishawakaIN Official Team Profile]
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===Team===
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Tell us about your team, your school!
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===Project===
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te_day=25&date_year=0&un=UNTIL IGEM JAMBOREE 2012&size=normal&mo=06&da=30&yr=2012" /><param
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What are you working on this semester?
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Arsenic is a naturally occurring toxic metal, and millions of people are exposed to this metalloid through contaminated drinking water worldwide. We propose to build an arsenic biosensor using the bacterial arsenic-sensitive promoter element, BBaK190015. In our previous experience, we have found that BBaK190015 has very high background activity and may be sensitive to tr...ace levels of arsenic and perhaps other metals such as copper. We unsuccessfully attempted to lower the sensitivity of BBaK190015 by isolating UV-resistant mutants and considered extending the 3’- end of the promoter to bind more ArsR inhibitors. However, we now propose to include the chemiluminescence as a reporter system. This will allow us to eliminate the background noise through the development of a battery powered iGlowi-I luminometer (Cambrigde 2010). We will design our device to have an internal standard for arsenic that should allow us to measure arsenic in the field.
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===Notebook===
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name="bgcolor" value="#f2f2f2" /></object>
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Show us how you spent your days.
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<br>
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<td valign="top">
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===Results/Conclusions===
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What did you achieve over the course of your semester?
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<td><a href="url">Home</a></td>
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<td><a href="https://2012hs.igem.org/Team:SouthBendMishawakaIN/Project">Project</a></td>
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===Safety===
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
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<td><a href="https://2012hs.igem.org/Team:SouthBendMishawakaIN/Notebook">Notebook</a></td>
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<td><a href="https://2012hs.igem.org/Team:SouthBendMishawakaIN/Safety">Safety</a></td>
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===Attributions===
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Who worked on what?
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<td><a href="https://2012hs.igem.org/Team:SouthBendMishawakaIN/Model">Model</a></td>
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<td><a href="https://2012hs.igem.org/Team:SouthBendMishawakaIN/Parts">Parts</a></td>
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===Human Practices===
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What impact does/will your project have on the public? 
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<td><a href="https://2012hs.igem.org/Team:SouthBendMishawakaIN/Sponsors">Sponsors</a></td>
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===Fun!===
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<h1>
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''"The Arsenator!"''
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</h1>
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<br>
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Millions of people worldwide are exposed to toxic levels of arsenic through
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contaminated
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drinking water. In last year’s project, we began the development of an E. coli-based
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<forum_subtle />
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arsenic biosensor using an arsenic-induced promoter from the Registry (K190015). Like the
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Groningen Team, we found that this promoter is “leaky,” having a constant low level of
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activity. We explored ways of reducing background activity to improve the sensitivity of
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 +
our device. We have built and characterized biosensors using K190015 and a number of
 +
 
 +
chemiluminescence generators from Vibrio fisheri and two species of fireflies. In order to
 +
 
 +
standardize our biosensor we have designed and built a simple luminometer. Paired with our
 +
 
 +
device, this can be used to inexpensively and rapidly detect arsenic in water, even by
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 +
people in underdeveloped parts of the world.
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</td>
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</tr>
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</table>
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</td>
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</tr>
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</table>
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</body>
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Latest revision as of 19:46, 1 October 2012

Topper Home.jpg


Welcome to the Homepage of the
DNA Architects
SouthBend MishawakaIN


Home
Project
Notebook
Safety
Model
Parts
Sponsors

''"The Arsenator!"''


Millions of people worldwide are exposed to toxic levels of arsenic through contaminated drinking water. In last year’s project, we began the development of an E. coli-based arsenic biosensor using an arsenic-induced promoter from the Registry (K190015). Like the Groningen Team, we found that this promoter is “leaky,” having a constant low level of activity. We explored ways of reducing background activity to improve the sensitivity of our device. We have built and characterized biosensors using K190015 and a number of chemiluminescence generators from Vibrio fisheri and two species of fireflies. In order to standardize our biosensor we have designed and built a simple luminometer. Paired with our device, this can be used to inexpensively and rapidly detect arsenic in water, even by people in underdeveloped parts of the world.

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