Team:Heidelberg LSL/Notebook-16/03/2012

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<h2> Biosensor Construction - 16/03/2012 </h2>
<h2> Biosensor Construction - 16/03/2012 </h2>
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*Miniprep of RecA-GFP clones #18 and #26 inocculated the previous
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**Measurement of DNA concentration with the Nanodrop gave 71 ng/ul for both constructs<br/>
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*Test digestion of 1 ug of #18 and #26 with EcoRI/NcoI
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<img src="https://static.igem.org/mediawiki/2012hs/2/2b/Test_dig_2_gel.png" />
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**Band correct for both constructs<br/>
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*All constructs (clones #1.18, 2.3, 3.4, 4.8) were send for sequencing @ GATC using standard biobrick sequencing primer VF2
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{{NotebookLower}}
{{NotebookLower}}

Revision as of 10:56, 9 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg



Notebook

Welcome to our notebook!

Here you will find the documentation of our laboratory work of the last few month in diary form. This notebook comprises the work in three phases:

            
Planning and
Development
            
            
Biosensor
Construction
              
            
Calibration and
Characterization
              

 

 

 

 

 

 

 

 

 

 

 

 

 

Biosensor Construction - 16/03/2012

  • Miniprep of RecA-GFP clones #18 and #26 inocculated the previous
    • Measurement of DNA concentration with the Nanodrop gave 71 ng/ul for both constructs


  • Test digestion of 1 ug of #18 and #26 with EcoRI/NcoI

<img src="Test_dig_2_gel.png" />

    • Band correct for both constructs


  • All constructs (clones #1.18, 2.3, 3.4, 4.8) were send for sequencing @ GATC using standard biobrick sequencing primer VF2



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